Development of new tools for kinomics

开发运动学新工具

• 批准号: BB/F003757/1
• 负责人: David Hodgson
• 金额: $ 10.95万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF003757%2F1
• 关键词: Development; new; tools; kinomics

This short term project aims to explore the viability of azidophosphate-based systems as tools for unravelling kinase-based signalling systems. Signals in biological systems are communicated via a process known as phosphorylation. In this process enzymes known as kinases pass on signals by transferring a small chemical entity (the phosphate group) from a molecule known as ATP to a signalling protein (kinase substrate). Once a protein has been phosphorylated, and only when it has been phosphorylated, it can bind to another receptor protein (phospho-protein recptor) and thus pass on the signal. Chemical and genetic methods have been developed for the identification kinase substrates, however the identification of phosphoprotein receptors is more challenging. Genetic methods have been devised, but these are complicated. Chemical methods are currently not available and we aim to fill this technology gap. All biological systems contained phosphorylation-mediated signalling pathways thus this new technology has a very broad potential user base which ranges from biomedical researches to basic science researchers.

这个短期项目的目的是探索叠氮磷酸盐为基础的系统的可行性，为解开激酶为基础的信号系统的工具。生物系统中的信号通过一个称为磷酸化的过程进行交流。在这个过程中，被称为激酶的酶通过将一个小的化学实体（磷酸基团）从一个被称为ATP的分子转移到一个信号蛋白（激酶底物）来传递信号。一旦蛋白质被磷酸化，并且只有当它被磷酸化时，它才能与另一种受体蛋白（磷酸蛋白受体）结合，从而传递信号。化学和遗传学方法已被开发用于鉴定激酶底物，然而磷蛋白受体的鉴定更具挑战性。遗传学方法已经被设计出来，但这些方法很复杂。目前还没有化学方法，我们的目标是填补这一技术空白。所有生物系统都含有磷酸化介导的信号通路，因此这项新技术具有非常广泛的潜在用户群，范围从生物医学研究到基础科学研究。

项目成果

5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 Rna polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs.

• DOI: 10.1080/15257770.2013.851393
• 发表时间: 2013
• 期刊: Nucleosides, nucleotides & nucleic acids
• 作者: Skipsey M;Hack G;Hooper TA;Shankey MC;Conway LP;Schröder M;Hodgson DR
• 通讯作者: Hodgson DR