A Chemo-Enzymatic Approach Towards Gamma-Thio-Nucleoside-5'-Triphosphates

研究背景我们的方法将提供一条更简单的路线来获得核苷-5‘-三磷酸盐(NTPs)，这是一系列生物技术和基础研究应用中的关键分子。目前主要有两种方法。前者对生物学家来说很容易获得，但只提供少量的材料，成本很高。另一种需要训练有素的化学家提供材料，而这种材料的化学成分具有挑战性，而且往往不可靠。我们的方法代表了一种新的生物技术目的和目标我们的目标是提供两种相关的更简单的方法，这两种方法应该允许生物学家以相对容易的方式获得所需的NTP，并允许他们以经济高效的方式获得更多有用的这些材料。我们的目标集中在使用一种酶系统，即核苷二磷酸激酶(NDPK)，以促进NTP的生产。潜在的应用和优势我们将在这份为期一年的概念验证提案中讨论的特定类别的NTP在确定酶如何工作以及信号通路如何在生物系统中运行方面有一系列的应用。我们的方法将使这些领域的研究人员和编制NTP的专业化学公司受益。随着我们的概念得到证实，我们希望将我们的战略扩展到更广泛的核苷酸系统，并针对这类分子产生一个通用的、广泛的平台。

Context of Proposed ResearchOur methods will give a simpler route towards nucleoside-5'-triphosphates (NTPs), which are key molecules in a range of biotechnological and basic research applications. Currently there are two main approaches. The first is readily accessible to biologists, but offers only small amount of material at high cost. The other requires highly trained chemists to provide the materials, where the chemistry is challenging and often unreliable. Our approach represents a new biotechnologyAims and ObjectivesWe aim to provide two related simpler approaches that should allows biologists to access the desired NTPs with relative easy in also allow them to access more useful amounts of these materials in a cost effective manner. Our objectives centre on using an enzyme system, nucleoside diphosphate kinase (NDPK), to facilitate the production of NTPs. Potential Applications and BenefitsThe specific class of NTP that we will address in this one-year proof-of-concept proposal has a range of applications in determining how enzymes work and also how signaling pathway operate within biological systems. Our approach will benefit research workers in these areas and the specialist chemical companies that prepare NTPs. With our concept proven, we hope to expand our strategy towards a broader range of nucleotide systems, and generate a generic, broad platform towards this class of molecule.