The role of tropomyosin in regulating actin filament dynamics in fission yeast

原肌球蛋白在调节裂殖酵母肌动蛋白丝动力学中的作用

• 批准号: BB/F011784/1
• 负责人: Daniel Mulvihill
• 金额: $ 44.66万
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF011784%2F1
• 关键词: role; tropomyosin; regulating; actin; filament

Actin is an essential cytoskeletal protein which is conserved in all eukaryotic organisms examined to date. It is actin's ability to polymerise into dynamic filaments which allows a cell's growth and even movement to be rapidly affected by both intra- and extra-cellular demands. Upon cellular signalling the actin polymerises to form filamentous or F-actin which form both cables and lattice structures, known as patches in yeasts. These actin cables have been shown to have a role in a large number of cellular processes which include cell polarity; cytokinesis; cell growth and movement; providing cortical tension; endocytosis; and acting as 'pathways' along which molecular motors (myosins) can travel. Actin's functions are conserved within yeasts, and forms cables and patches which localise predominantly to regions of cell growth, facilitating the cells increase in size during interphase, or division during cytokinesis. The fission yeast, Schizosaccharomyces pombe, is cylindrical in shape, with growth occurring in a polarised manner at the cell pole. Actin is seen to localised predominantly to patch structures at these growing cell poles and to actin cables throughout the cytoplasm during interphase. During mitosis actin cables exist as a major component of the cytokinetic ring, which contracts in order for a cell to divide. This project make use of the cross-discipline approaches allowed by this experimentally tractable organism to determine the role actin filaments play in regulating and maintaining polarised cell growth. Using the versatile fission yeast model system allows us to not only examine actin filaments in both an in vivo and in vitro context, but allows us to make use of mutants to modulate filaments' dynamics as well as their ability to interact with motor proteins. Thus it will be possible to elucidate their function(s) within the cell.

肌动蛋白是一种重要的细胞骨架蛋白，在所有的真核生物中都是保守的。正是肌动蛋白聚合成动态细丝的能力，使得细胞的生长甚至运动迅速受到细胞内和细胞外需求的影响。在细胞信号传导后，肌动蛋白聚合形成丝状或F-肌动蛋白，其形成电缆和晶格结构，称为酵母中的斑块。这些肌动蛋白电缆已被证明在大量的细胞过程中发挥作用，包括细胞极性;胞质分裂;细胞生长和运动;提供皮质张力;内吞作用;以及作为分子马达（肌球蛋白）可以行进的沿着“通路”。肌动蛋白的功能在酵母中是保守的，并形成主要定位于细胞生长区域的电缆和补丁，促进细胞在间期期间的大小增加，或在胞质分裂期间的分裂。裂殖酵母，粟酒裂殖酵母，是圆柱形的，在细胞极以极化的方式生长。肌动蛋白被认为是本地化的主要补丁结构在这些不断增长的细胞极和肌动蛋白电缆整个细胞质在间期。在有丝分裂期间，肌动蛋白电缆作为细胞动力学环的主要组成部分存在，其收缩以使细胞分裂。这个项目利用跨学科的方法，允许这个实验上听话的生物体，以确定肌动蛋白丝在调节和维持极化细胞生长的作用。使用多功能的裂变酵母模型系统，使我们不仅可以检查肌动蛋白丝在体内和体外的情况下，但允许我们利用突变体来调节丝的动力学，以及他们的能力，与马达蛋白相互作用。因此，有可能阐明它们在细胞内的功能。

项目成果

Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer.

裂变酵母菌菌素中温度敏感的点突变对肌动蛋白 - 肌球蛋白共聚物的稳定性和功能具有长度的影响。

• DOI: 10.1016/j.bbrc.2017.10.109
• 发表时间: 2018-11-25
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Johnson CA;Brooker HR;Gyamfi I;O'Brien J;Ashley B;Brazier JE;Dean A;Embling J;Grimsey E;Tomlinson AC;Wilson EG;Geeves MA;Mulvihill DP
• 通讯作者: Mulvihill DP

Targeted amino-terminal acetylation of recombinant proteins in E. coli.

• DOI: 10.1371/journal.pone.0015801
• 发表时间: 2010-12-23
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Johnson M;Coulton AT;Geeves MA;Mulvihill DP
• 通讯作者: Mulvihill DP

The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast

• DOI: 10.1242/jcs.069971
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Coulton, Arthur T.;East, Daniel A.;Mulvihill, Daniel P.
• 通讯作者: Mulvihill, Daniel P.