柑橘木虱是柑橘毁灭性病害-黄龙病（Huanglongbing, HLB）的主要媒介宿主，HLB病菌能侵染柑橘木虱并在其体内繁殖生长。项目组在前期研究中已经明确了HLB病菌侵染会影响柑橘木虱的原肌球蛋白（Tropomyosin, Tm）和ATP synthase亚基等蛋白变化，同时在带菌木虱中发现了HLB病菌GroEL蛋白大量表达。本研究拟进一步解析HLB病菌侵染柑橘木虱引起宿主Tm和ATP synthase亚基变化机制以及菌体GroEL介导的病菌在木虱体内的转运过程。项目拟采用亚细胞BN PAGE技术鉴定参与HLB病菌和Tm互作的蛋白复合体，采用2-D电泳结合Far-western技术在亚细胞水平上筛选与菌体GroEL互作的柑橘木虱蛋白，辅助以免疫病理学观察分析和转录水平上的验证。希望研究获得Tm、GroEL和ATP synthase在HLB病菌入侵柑橘木虱以及细胞内转运的信息，为进一步研究HLB防治新策略、控制媒介昆虫的病原传播提供理论依据。

Diaphorina citri (Hemiptera: Psyllidae) is the major vector that transmits Huanglongbing（HLB）,which is a devastating disease of citrus caused by ‘Candidatus Liberibacter asiaticus’ (CaLas). We have found Tm and ATP synthase subunit of citrus psyllid produced changes under HLB pathogen infection in previous studies.Meanwile, vast GroEL of CaLas was found in subcell of citrus psyllid. Our project is aimed to study on the interaction protein between CaLas with Tm and ATP synthase subunit of host, and research the role of GroEL in the process CaLas infected psyllid. BN PAGE will used to identify interaction protein complex with Tm under CaLas infected.2-DE combined Far-western will used to screen out interaction protein with bacterial GroEL in psyllid subcell. Meanwhile, the project will carry out Immune pathology observation and verify gene in the transcription. Our perspective will reveal molecular mechanism of Tm、GroELand ATP synthase involved in the CaLas infection the citrus psyllid and inner transfer of subcell, and will find CLas combined targets to get a new strategy of control HLB. The results will also benefit to support for studying on interaction pathogen between insect hosts and control the transmission of the pathogen.