TELOMERE-DEPENDENT HETEROCHROMATIC EXPRESSIONSITE SILENCING IN T BRUCEI

布鲁斯锥虫中端粒依赖性异染色质表达位点沉默

• 批准号: 7355147
• 负责人: GEORGE ALAN MARTIN CROSS
• 金额: $ 0.12万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355147
• 关键词: TELOMERE; DEPENDENT; HETEROCHROMATIC; EXPRESSIONSITE; SILENCING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To better understand the role of telomere-dependent heterochromatic expression site silencing in T. brucei, I hope to identify telomere-specific histone modifications and to test their role in silencing of expression sites in procyclic form T. brucei. To achieve this goal, I will try to enrich for telomeric histones using co-IP techniques developed in the Chait and Rout laboratories. Subsequently, I hope to use mass-spectrocsopy to analyze telomeric histones for characteristic modification.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。为了更好地了解端粒依赖的异染色质表达位点沉默在布氏毛滴虫中的作用，我希望识别端粒特异性的组蛋白修饰，并测试它们在原环型布氏毛滴虫表达位点沉默中的作用。为了实现这一目标，我将尝试使用Chait和Rout实验室开发的联合IP技术来丰富端粒组蛋白。随后，我希望利用质谱学对端粒组蛋白进行特征修饰。