ID OF MINIBRAIN KINASE/DUAL-SPECIFICITY YAK 1-RELATED KINASE 1A P-SITES IN DYN-

DYN- 中小脑激酶/双特异性牦牛 1 相关激酶 1A P 位点的 ID

• 批准号: 7355062
• 负责人: Rong Wang
• 金额: $ 0.12万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355062
• 关键词: ID; MINIBRAIN; KINASE; DUAL; SPECIFICITY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The minibrain kinase (Mnbk)/dual specificity Yak 1 related kinase (Dyrk1A) gene is localized in the q21.22 region of human chromosome 21 and it has been associated with the mental retardation of Down syndrome. Mnbk/Dyrk1A is a proline-directed Ser/Thr kinase which preferentially phosphorylates the sequence RPX(S/T)P. Previously, Mnbk/Dyrk1A was found to phosphorylate dynamin 1 and alter its interactions with SH3 domain-containing proteins (Chen-Hwang, M. C. et. al. J. Biol. Chem. 277: 17597-17604, 2002). In this study, we sought to determine the Mnbk/Dyrk1A phosphorylation sites in dynamin 1. By a combination of dynamin 1 deletion mutants and synthetic peptides, three potential Mnbk/Dyrk1A phosphorylation sites, Ser-778, -795 and -857, were identified. Phosphorylation at Ser-795 and -857 was subsequently confirmed in Mnbk/Dyrk1A-phosphorylated intact dynamin 1 by mass spectroscopy. A manuscript has been submitted for publication.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。小脑激酶（minibrain kinase，Mnbk）/双特异性Yak 1相关激酶（dual specificity Yak 1 related kinase，Dyrk 1A）基因定位于人类21号染色体q21.22区域，与唐氏综合征（Down syndrome）的智力低下相关。Mnbk/Dyrk 1A是脯氨酸导向的Ser/Thr激酶，其优先磷酸化序列RPX（S/T）P。以前，发现Mnbk/Dyrk 1A磷酸化发动蛋白1并改变其与含SH 3结构域的蛋白的相互作用（Chen-Hwang，M. C. et. J.Biol.Chem.277：17597-17604，2002）。在这项研究中，我们试图确定发动蛋白1中的Mnbk/Dyrk 1A磷酸化位点。通过dynamin 1缺失突变体和合成肽的组合，鉴定了三个潜在的Mnbk/Dyrk 1A磷酸化位点，Ser-778，-795和-857。随后通过质谱法在Mnbk/Dyrk 1A-磷酸化的完整发动蛋白1中证实了Ser-795和-857的磷酸化。一份手稿已提交出版。