PROTEOMEX LTQ WORKSTATION SHARED INSTRUMENTATION: DIABETES,AUTOIMMUN THYROID DIS

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本建议书要求一台Finnigan ProteomeX LTQ工作站和一台集成色谱电喷雾电离线性离子陷阱质谱仪，以使我们的基础和翻译蛋白质组学中心/共享研究设施的分析能力达到最先进的水平，并提高我们的能力，以满足我们研究人员不断增加的工作量和强度。这一要求对于支持我们正在进行的研究计划以及在蛋白质鉴定、翻译后修饰表征、基因发现和功能蛋白质组学方面的新的合作研究计划至关重要。目前，西奈山医疗中心(医院和医学院)没有ESI-LTQ线性离子陷阱质谱仪，这是提出这一要求的根本原因。我们需要使用ProteomeX LTQ工作站来取代我们的老旧的Finnigan LCQ经典质谱仪，因为它经常出现故障，严重影响正在进行的研究项目。所需的ProteomeX LTQ线性离子陷阱质谱仪将安装在我们的基础蛋白质组学和翻译蛋白质组学中心。购买ProteomeX LTQ线性离子陷阱质谱仪是继续扩大和加强西奈山园区人类疾病基础研究计划和翻译蛋白质组学研究的基础。如本申请所述，来自10个不同部门的22名以上NIH资助的研究人员和来自邻近研究所的两名NIH资助的研究人员对这台质谱仪有特定的需求，这将是一个共享的研究资源。事实上，由于校园内缺乏高度灵敏和高通量的蛋白质组学仪器，其中一些研究项目严重延误或受到影响。因此，购买ProteomeX LTQ系统将对我们研究所的研究能力产生重大影响

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests a Finnigan ProteomeX LTQ workstation, an integrated chromatography electrospray ionization linear ion trap mass spectrometer to bring our Basic and Translational Proteomics Center / Shared Research Facility's analytical capability up to state-of-the-art and to increase our capacity to meet the ever increasing volume and intensity of work by our investigators. This request is essential to support our ongoing research programs and to stimulate and enable new collaborative research initiatives on protein identification, posttranslational modification characterization, gene discovery, and functional proteomics. Currently, there is no ESI-LTQ linear ion trap mass spectrometer at the Mount Sinai Medical Center (Hospital and School of Medicine), which provides a fundamental reason for this request. The ProteomeX LTQ workstation is requested in replace of our aged Finnigan LCQ classic mass spectrometer which breaks down so frequently that it seriously affects the on-going research projects. The requested ProteomeX LTQ linear ion trap mass spectrometer will be housed in our Basic and Translational Proteomics Center. The purchase of the ProteomeX LTQ linear ion trap mass spectrometer is fundamental to the continuing expansion and enhancement of the basic research programs and translational proteomics research for human disease on the Mount Sinai campus. As described in this application, over 22 NIH-funded investigators from 10 different departments and two NIH-funded investigators from neighboring institutes have specific needs for this mass spectrometer, which will be a shared research resource. Indeed, due to the lack of an highly sensitive and high throughput proteomics instrument on campus, some of these research projects are seriously delayed or compromised. Thus, the purchase of the ProteomeX LTQ system will have a major impact on the research capabilities at our instituti