Understanding the molecular dynamics of NHEJ-mediated synapsis

了解 NHEJ 介导的突触的分子动力学

基本信息

批准号：
BB/F013795/1
负责人：
Aidan Doherty
金额：
$ 104.26万
依托单位：
University of Sussex
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FF013795%2F1
关键词：
Understanding molecular dynamics NHEJ mediated

项目摘要

Our cells contain DNA, the so called 'genetic blueprint of life' which encodes the information for our genes. DNA has a simple repeating structure composed of two complementary strands of DNA which form a double-helix structure. The integrity of DNA is constantly being challenged by various DNA-damaging agents. These agents include high energy UV and X-ray radiation from the sun, chemicals both man-made and environmental and even the oxygen we breathe can attack and damage DNA. In recent years it has been realised that our own cells produce a large number of proteins responsible for repairing this DNA damage which, if left unchecked, would lead to the development of conditions such as cancer. These protein 'machines' can cut out and replace aberrant DNA mutations/structures and splice together broken DNA strands. One such protein is called DNA ligase, which interacts with a partner protein, Ku. These proteins are able to detect physical breaks in the DNA helices, bring the ends back together and reseal these breaks thus restore the double-strand integrity of the DNA. The focus of this work is to understand how these proteins can bridge the break and make the DNA ends come back together prior to the sealing step.

我们的细胞包含DNA，即所谓的“生命的遗传蓝图”，它编码我们基因的信息。 DNA具有简单的重复结构，由DNA的两个互补链组成，该结构形成双螺旋结构。 DNA的完整性不断受到各种DNA损害剂的挑战。这些试剂包括来自太阳的高能量紫外线和X射线辐射，人造和环境化学物质，甚至我们呼吸的氧气都会攻击和损坏DNA。近年来，人们已经意识到我们自己的细胞会产生大量负责修复这种DNA损伤的蛋白质，如果不受组织检查，这将导致癌症等疾病的发展。这些蛋白质的“机器”可以切出并替换异常的DNA突变/结构，并剪接碎DNA链。一种这样的蛋白质称为DNA连接酶，它与伴侣蛋白KU相互作用。这些蛋白质能够检测到DNA螺旋中的物理断裂，将末端重新恢复并重新密封这些断裂，从而恢复了DNA的双链完整性。这项工作的重点是了解这些蛋白质如何弥合断裂并使DNA末端在密封步骤之前恢复在一起。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria.

DOI：
10.1038/s41467-017-01365-y
发表时间：
2017-11-01
期刊：
Nature communications
影响因子：
16.6
作者：
Płociński P;Brissett NC;Bianchi J;Brzostek A;Korycka-Machała M;Dziembowski A;Dziadek J;Doherty AJ
通讯作者：
Doherty AJ

CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation.