权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Understanding the molecular dynamics of NHEJ-mediated synapsis

了解 NHEJ 介导的突触的分子动力学

基本信息

批准号：
BB/F013795/1
负责人：
Aidan Doherty
金额：
$ 104.26万
依托单位：
University of Sussex
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FF013795%2F1
关键词：
Understanding molecular dynamics NHEJ mediated

项目摘要

Our cells contain DNA, the so called 'genetic blueprint of life' which encodes the information for our genes. DNA has a simple repeating structure composed of two complementary strands of DNA which form a double-helix structure. The integrity of DNA is constantly being challenged by various DNA-damaging agents. These agents include high energy UV and X-ray radiation from the sun, chemicals both man-made and environmental and even the oxygen we breathe can attack and damage DNA. In recent years it has been realised that our own cells produce a large number of proteins responsible for repairing this DNA damage which, if left unchecked, would lead to the development of conditions such as cancer. These protein 'machines' can cut out and replace aberrant DNA mutations/structures and splice together broken DNA strands. One such protein is called DNA ligase, which interacts with a partner protein, Ku. These proteins are able to detect physical breaks in the DNA helices, bring the ends back together and reseal these breaks thus restore the double-strand integrity of the DNA. The focus of this work is to understand how these proteins can bridge the break and make the DNA ends come back together prior to the sealing step.

我们的细胞含有DNA，即所谓的“生命的遗传蓝图”，它为我们的基因编码信息。DNA具有简单的重复结构，由两条互补的DNA链组成，形成双螺旋结构。DNA的完整性不断受到各种DNA破坏剂的挑战。这些物质包括来自太阳的高能紫外线和X射线辐射，人造和环境的化学物质，甚至我们呼吸的氧气都可以攻击和破坏DNA。近年来，人们已经意识到，我们自己的细胞产生大量的蛋白质，负责修复这种DNA损伤，如果不加以控制，将导致癌症等疾病的发展。这些蛋白质“机器”可以切割和替换异常的DNA突变/结构，并将断裂的DNA链拼接在一起。其中一种蛋白质称为DNA连接酶，它与伴侣蛋白Ku相互作用。这些蛋白质能够检测DNA螺旋中的物理断裂，将末端重新连接在一起并重新密封这些断裂，从而恢复DNA的双链完整性。这项工作的重点是了解这些蛋白质如何弥合断裂，并使DNA末端在密封步骤之前重新结合在一起。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria.

DOI：
10.1038/s41467-017-01365-y
发表时间：
2017-11-01
期刊：
Nature communications
影响因子：
16.6
作者：
Płociński P;Brissett NC;Bianchi J;Brzostek A;Korycka-Machała M;Dziembowski A;Dziadek J;Doherty AJ
通讯作者：
Doherty AJ

CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation.