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Novel fluorescent sensors for simple, sensitive, and specific protein detection

用于简单、灵敏和特异性蛋白质检测的新型荧光传感器

基本信息

批准号：
8527772
负责人：
SAMIE R JAFFREY
金额：
$ 32.13万
依托单位：
WEILL MEDICAL COLL OF CORNELL UNIV
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8527772
关键词：
Antibodies Basic Science Binding Biological Biological Assay Biological Markers Biomedical Research Calcium Cell division Cells Chemicals Clinical Communities Complex Cyclic AMP Degradation Pathway Detection Development Docking Enzyme-Linked Immunosorbent Assay Exposure to Fluorescence Resonance Energy Transfer Gel Generations Genes Genetic Enhancer Element Goals Hospitals Immunofluorescence Immunologic In Vitro Label Laboratories Laboratory Research Lead Life Malignant Neoplasms Measurement Measures Medicine Methodology Methods MicroRNAs Microarray Analysis Microscopic Monitor Nobel Prize Oligonucleotides Pattern Pharmaceutical Preparations Phosphatidylinositol 4,5-Diphosphate Positioning Attribute Preclinical Drug Evaluation Process Proteins Proteomics Radioimmunoassay Reagent Relative (related person)Research Research Design Research Project Grants Resources Sampling Science Serum Set protein Techniques Technology Testing Time Tissue Sample Transgenes Western Blotting assay development base cell fixation cell growth design drug development high throughput screening in vivo novel overexpression programs promoter protein expression protein protein interaction research clinical testing research study response screening sensor small molecule tool

项目摘要

ABSTRACT The detection of proteins is fundamental to essentially all biomedical research. Current strategies to detect proteins include techniques such as Western blotting and enzyme-linked immunosorbent assays. However, developing assays for specific proteins is time consuming and a bottleneck in high throughput screen development, biomarker characterization, and many basic science research projects. Detecting proteins in cells is similarly difficult, and typically requires sacrificing the cell by fixation followed by immunofluorescence assays. Currently, there are no techniques that permit the levels of endogenously expressed proteins to be monitored in real time in living cells. The goal of this project is to develop a generalizable and simple method to detect proteins in an in vitro and in vivo setting. We have developed a novel class of oligonucleotide-based sensors, and we have demonstrated that these sensors permit the fluorometric detection of specific analytes. The sensor functions by converting an otherwise nonfluorescent molecule into a fluorescent configuration upon analyte binding. We propose to develop a generalizable method that would allow sensors to be developed for virtually any protein. We describe experiments that involve expressing genetically encoded sensors within cells to detect specific endogenous proteins during cell growth and division. This approach has the potential to be vastly more versatile that current fluorescence resonance energy transfer (FRET)-based genetically encodable probes. We also describe a simple approach to generate protein sensing microarrays using these sensors. In this technique, biological samples will not require sample processing such as chemical derivatization with a fluorescent tag. This label-free approach has the potential to provide a simple methodology for proteomic analyses of tissue samples. We also describe the use of these sensors for in vitro protein quantification. Together, the experiments described in this application describe a versatile sensor technology that will introduce fundamentally novel and widely useful technologies to the entire biomedical research community.

摘要蛋白质的检测基本上是所有生物医学研究的基础。目前检测蛋白质的策略包括蛋白质印迹和酶联免疫吸附测定等技术。然而，开发针对特定蛋白质的测定是耗时的，并且是高通量筛选开发、生物标志物表征和许多基础科学研究项目中的瓶颈。检测蛋白质细胞的方法同样困难，并且通常需要通过固定然后进行免疫荧光测定来牺牲细胞。目前，还没有允许在活细胞中真实的实时监测内源性表达蛋白质水平的技术。本项目的目标是开发一种可推广的和简单的方法来检测蛋白质在体外和体内设置。我们已经开发了一种新型的基于磷脂的传感器，我们已经证明，这些传感器允许特定分析物的荧光检测。该传感器通过在分析物结合时将原本非荧光的分子转换成荧光构型来起作用。我们建议开发一种可推广的方法，使传感器开发几乎任何蛋白质。我们描述了涉及表达基因编码的传感器的实验，在细胞生长和分裂过程中检测特定的内源性蛋白质。这种方法具有比目前基于荧光共振能量转移（FRET）的遗传编码探针更通用的潜力。我们还描述了一种简单的方法来产生蛋白质传感微阵列使用这些传感器.在该技术中，生物样品将不需要样品处理，例如用荧光标记进行化学衍生。这种无标记的方法有可能为组织样品的蛋白质组学分析提供一种简单的方法。我们还描述了使用这些传感器在体外蛋白质定量。总之，本申请中描述的实验描述了一种多功能传感器技术，其将从根本上将新颖且广泛有用的技术引入整个生物医学领域。研究社区。