CHEMOPREVENTIVE ISOFLAVONES FROM MICROORGANISMS

赋予花和果实颜色的植物性物质， 它们被包含在正常的饮食中。 黑猩猩是一类 大豆中发现的黄酮类化合物。 流行病学研究表明， 饮食中富含大豆的地理区域， 乳腺癌发病率染料木黄酮是一种致癌物质， 大豆与化学预防作用有关。 染料木黄酮是 也是拓扑异构酶II和蛋白酪氨酸激酶的抑制剂。 因为这两种酶参与细胞分化， 除了增殖外，染料木黄酮还可以具有化学治疗性质。 急需的生物医学研究的染料木素目前受到抑制， 合成生产或提取的纯化合物的高成本 大豆。 据报道，微生物还可以产生 染料木黄酮，并可提供该化合物的经济来源。 微生物发酵的特点是易于加工 通过遗传和生理操作进行改良。 同相 我们建议确认微生物产生的异黄酮，并开始 开发基因和生化分析所需的工具， 生产。 在第二阶段，我们希望建立一个高效的 发酵或微生物转化方法和用途 遗传学，以产生新的生物测试的类似物。

Flavonoids ar plant substances that give flowers and fruits their color, and they are included in a normal diet. The isoflavones are a class of flavonoids found in soybean. Epidemiological studies have shown that in geographic areas where diets are rich in soybeans there is a reduced incidence of breast cancer Genistein is an isoflavone whose presence in soybeans has been linked to the chemopreventive effect. Genistein is also an inhibitor of topoisomerase II and protein tyrosine kinase. Because these two enzymes are involved in cellular differentiation and proliferation, genistein could also have chemotherapeutic properties. Much needed biomedical research on geneistein is currently inhibited by the high cost of the pure compound synthetically produced or extracted from soybeans. Microorganisms have also been reported to produce genistein and could provide an economical source for the compound. Microbial fermentations are characteristically amenable to process improvement through genetic and physiological manipulations. In phase one we propose to confirm microbial production of isoflavones and begin to develop the tools needed for genetic and biochemical analysis of isoflavone production. In phase 2, we hope to create an efficient fermentation or microbial transformation process and use targeted genetics to produce new isoflavone analogs for biological testing.