HORMONES AND AGING--HYPOTHALAMIC-PITUITARY FUNCTION IN EXPERIMENTAL ANIMALS

许多激素分泌和组织对激素的反应依赖于 细胞膜受体转导在老年动物中是钝化的， 与年轻的动物相比。 荷尔蒙反应的这种变化是 通常与刺激物水平或比率的改变有关， GTP结合蛋白（Gs）和抑制性GTP结合蛋白（Gi）， 介导许多细胞内的膜结合受体反应。 我们 （1）研究衰老对人类健康的影响。 脂肪环化酶介导的儿茶酚胺受体的G蛋白组分 细胞和肝细胞膜，和（2）研究衰老对 EF-1 α是一种G蛋白，是肽的关键介质， 在核糖体的延长，以生长激素和睾酮， 骨骼肌和其他组织。 今年，我们设计并 用于测定EF-1量的经验证的蛋白质印迹法 在 来自不同年龄的激素缺乏和激素处理动物的组织 并开发了EF-1 alpha的表达载体，以便 产生纯EF-1 α标准品。

Many hormone secretion and tissue responses to hormones that depend on cell membrane receptor transduction are blunted in old animals when compared to young animals. Such changes in hormonal responses are frequently associated with alterations in the levels or ratios of stimulato GTP-binding proteins (Gs) and inhibitory GTP binding proteins (Gi ) which mediate many membrane-bound receptor responses intracellularly. We have been (1) conducting investigations of the effects of aging on the G-protein components of cyclase mediated catecholamines receptors in fat cell and hepatic cell membranes, and (2) studying the effects of aging on responses of EF-1alpha, a G protein which is a key mediator of peptide elongation at the ribosome s to growth hormone and testosterone in skeletal muscle and other tissues. This year, we have devised and validated Western blot assay for determining the quantity of EF-1 in tissue from hormone deficient and hormone treated animals of various ages and have developed an expression vector for EF-1alpha in order to produce a pure EF-1alpha standard.