HORMONES AND AGING--HYPOTHALAMIC-PITUITARY FUNCTION IN EXPERIMENTAL ANIMALS

许多激素分泌和组织对激素的反应依赖于 在老年动物中，细胞膜受体转导被钝化 与年幼的动物相比。荷尔蒙反应的这种变化是 经常与刺激水平或比率的变化有关 GTP结合蛋白(Gs)和抑制性GTP结合蛋白(GI) 在细胞内介导许多膜结合的受体反应。我们 一直在(1)调查衰老对老年人的影响 脂肪中儿茶酚胺受体环化酶介导的G蛋白成分 细胞和肝细胞膜，以及(2)研究衰老对 多肽的关键介质--G蛋白EF-1α的反应 核糖体S对生长激素和睾酮的延长 骨骼肌和其他组织。今年，我们设计了和 经验证的免疫印迹法检测血浆中EF-1的含量 不同年龄段激素缺乏和激素处理动物的组织 并开发了EF-1α的表达载体，以便 制作一个纯EF-1阿尔法标准。

Many hormone secretion and tissue responses to hormones that depend on cell membrane receptor transduction are blunted in old animals when compared to young animals. Such changes in hormonal responses are frequently associated with alterations in the levels or ratios of stimulato GTP-binding proteins (Gs) and inhibitory GTP binding proteins (Gi ) which mediate many membrane-bound receptor responses intracellularly. We have been (1) conducting investigations of the effects of aging on the G-protein components of cyclase mediated catecholamines receptors in fat cell and hepatic cell membranes, and (2) studying the effects of aging on responses of EF-1alpha, a G protein which is a key mediator of peptide elongation at the ribosome s to growth hormone and testosterone in skeletal muscle and other tissues. This year, we have devised and validated Western blot assay for determining the quantity of EF-1 in tissue from hormone deficient and hormone treated animals of various ages and have developed an expression vector for EF-1alpha in order to produce a pure EF-1alpha standard.