Core C: Molecualr Imaging High Throughput Screening Core (HTS)
核心 C:分子成像高通量筛选核心 (HTS)
基本信息
- 批准号:7287036
- 负责人:
- 金额:$ 18.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-09-28 至 2011-12-31
- 项目状态:已结题
- 来源:
- 关键词:ABCB1 geneBiological AssayBioluminescenceBlood CirculationCancer CenterCell Cycle RegulationCell DeathCellsCellular biologyChemistryCommunitiesCultured CellsDataEndopeptidasesEnzyme Inhibitor DrugsEnzyme InhibitorsFacility Construction Funding CategoryFundingGene ExpressionGenomeHousingHumanHuman ResourcesImageImage AnalysisInstitutesLibrariesLiquid substanceLuciferasesMedicalMolecularMolecular BankMolecular BiologyNotch Signaling PathwayPeptide HydrolasesPeptidesPharmaceutical PreparationsPharmacologyPhosphoric Monoester HydrolasesPhosphotransferasesProcessProtocols documentationRadiology SpecialtyRangeRegulationReporterResearch PersonnelResearch Project GrantsResource DevelopmentResourcesRobotRoboticsScienceScreening procedureSignal TransductionSiteSmall Interfering RNASpecialized CenterTNFRSF5 geneTherapeuticUnited States National Institutes of HealthUniversitiesWashingtonair filtercell growthcellular engineeringdayfallshigh throughput screeningin vivo Cellular and Molecular Imaging Centersinnovationinstrumentknock-downmedical schoolsmolecular imagingnovelpressureprotein phosphatase inhibitor-2protein protein interactionresearch and developmentresearch studyresponsesmall moleculesmall molecule librariesubiquitin-protein ligase
项目摘要
A.3.iii. High Throughput Screening Core (HTS)
In response to the NIH Roadmap to Molecular Libraries and Imaging, we have fully established a
P50 High Throughput Core. The new Core represents a multi-departmental effort with fiscal support for
establishing the Core coming from P50 funds in combination with the Departments of Radiology, Molecular
Biology & Pharmacology, and Cell Biology as well as the Howard Hughes Medical Institute. The Dean of
Washington University School of Medicine as well as the Director of the Washington University Siteman
Cancer Center joined our effort with the commitment of additional funds for further development of the
resource. This Core serves as an outstanding on-going example of how our P50 program has
leveraged university resources and continues to stimulate interdisciplinary molecular imaging
activity throughout the WU campus. We have optimized several robotic protocols and logistical
requirements for our first projects in high throughput screening and exciting data are now coming on-line
during the summer of 2006. The Core allows for automated screening of cells cultured in 96 or 384 plate
formants and can be applied to multiple investigator-initiated molecular imaging applications throughout the
university community. The applications include:
1) small molecule screens applied to cells engineered by WU investigators for imaging and
therapeutic drug leads.
2) small molecule or peptide screens to identify enzyme inhibitors (directed against high priority
proteases, kinases, phosphatases, etc.), or modulators of protein-protein interactions.
3) genome-wide siRNA library screens against kinases, phosphatases and E3-ligases in human
cells targeting signal transduction, cell growth or cell death responses.
Our first projects are focused on use of the novel split luciferase complementation imaging platform
developed in the Molecular Reporter Core for analysis of protein-protein interactions using siRNA libraries
against all known expressed human kinases and phosphatases. Knock-down strategies are also being
used to explore fusion reporters for bioluminescence readouts of cell cycle regulation, the Notch signaling
pathway, and regulation of MDR1 gene expression and other related discovery projects. We will soon be
incorporating small molecule libraries from the Chemistry Core. These directly demonstrate the cycle of
synergies between the Molecular Reporter Core, the Chemistry Core, the HTS Core and ICMIC R&D
Projects.
A.3.iii.高吞吐量筛选堆芯(HTS)
为了响应NIH分子文库和成像路线图,我们已经完全建立了一个
P50高功率核心。新的核心是多部门的努力,并得到财政支持,
建立核心来自P50基金与放射科,分子
生物学与药理学、细胞生物学以及霍华德休斯医学研究所。院长
华盛顿大学医学院以及华盛顿大学研究中心主任
癌症中心加入了我们的努力,承诺提供额外资金,用于进一步发展
resource.这个核心作为一个杰出的持续的例子,我们的P50计划如何
利用大学资源,继续促进跨学科分子成像
在整个WU校园的活动。我们已经优化了几个机器人协议和后勤
我们在高通量筛选和令人兴奋的数据的第一个项目的要求现在即将上线
在2006年夏天。Core允许自动筛选在96或384平板中培养的细胞
共振峰,并可应用于多个闪烁器启动的分子成像应用,
大学社区这些应用包括:
1)小分子屏幕应用于由WU研究人员工程化的细胞进行成像,
治疗药物的线索。
2)小分子或肽筛选以鉴定酶抑制剂(针对高优先级
蛋白酶、激酶、磷酸酶等),或蛋白质-蛋白质相互作用的调节剂。
3)全基因组siRNA文库筛选人类激酶、磷酸酶和E3-连接酶
细胞靶向信号转导、细胞生长或细胞死亡应答。
我们的第一个项目集中在使用新的分裂荧光素酶互补成像平台
在Molecular Reporter Core中开发,用于使用siRNA文库分析蛋白质-蛋白质相互作用
针对所有已知表达的人激酶和磷酸酶。击倒战略也被
用于探索细胞周期调控的生物发光读数的融合报告基因,Notch信号传导
通路、MDR 1基因表达调控等相关发现项目。我们将很快
整合来自化学核心的小分子文库。这些直接证明了
分子报告核心、化学核心、HTS核心和ICMIC研发之间的协同作用
项目
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('HELEN M PIWNICA-WORMS', 18)}}的其他基金
Mechanisms of fasting-induced radioprotection of small intestinal epithelial cells
禁食诱导的小肠上皮细胞辐射防护机制
- 批准号:
10645872 - 财政年份:2023
- 资助金额:
$ 18.79万 - 项目类别:
Fasting Protects Small Intestinal Stem Cells from Lethal DNA Damage: Mechanistic Insight and Preclinical Translation
禁食可保护小肠干细胞免受致命性 DNA 损伤:机制洞察和临床前转化
- 批准号:
10090461 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
Fasting Protects Small Intestinal Stem Cells from Lethal DNA Damage: Mechanistic Insight and Preclinical Translation
禁食可保护小肠干细胞免受致命性 DNA 损伤:机制洞察和临床前转化
- 批准号:
9307224 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
8361353 - 财政年份:2011
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
8168703 - 财政年份:2010
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
7953916 - 财政年份:2009
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
7721480 - 财政年份:2008
- 资助金额:
$ 18.79万 - 项目类别:
Res Proj 2: Molecular Imaging Strategies to Study Cdc25A Regulation in vivo
Res Proj 2:研究 Cdc25A 体内调节的分子成像策略
- 批准号:
7287031 - 财政年份:2007
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
7355307 - 财政年份:2006
- 资助金额:
$ 18.79万 - 项目类别:
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