Core C: Molecualr Imaging High Throughput Screening Core (HTS)
核心 C:分子成像高通量筛选核心 (HTS)
基本信息
- 批准号:7287036
- 负责人:
- 金额:$ 18.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-09-28 至 2011-12-31
- 项目状态:已结题
- 来源:
- 关键词:ABCB1 geneBiological AssayBioluminescenceBlood CirculationCancer CenterCell Cycle RegulationCell DeathCellsCellular biologyChemistryCommunitiesCultured CellsDataEndopeptidasesEnzyme Inhibitor DrugsEnzyme InhibitorsFacility Construction Funding CategoryFundingGene ExpressionGenomeHousingHumanHuman ResourcesImageImage AnalysisInstitutesLibrariesLiquid substanceLuciferasesMedicalMolecularMolecular BankMolecular BiologyNotch Signaling PathwayPeptide HydrolasesPeptidesPharmaceutical PreparationsPharmacologyPhosphoric Monoester HydrolasesPhosphotransferasesProcessProtocols documentationRadiology SpecialtyRangeRegulationReporterResearch PersonnelResearch Project GrantsResource DevelopmentResourcesRobotRoboticsScienceScreening procedureSignal TransductionSiteSmall Interfering RNASpecialized CenterTNFRSF5 geneTherapeuticUnited States National Institutes of HealthUniversitiesWashingtonair filtercell growthcellular engineeringdayfallshigh throughput screeningin vivo Cellular and Molecular Imaging Centersinnovationinstrumentknock-downmedical schoolsmolecular imagingnovelpressureprotein phosphatase inhibitor-2protein protein interactionresearch and developmentresearch studyresponsesmall moleculesmall molecule librariesubiquitin-protein ligase
项目摘要
A.3.iii. High Throughput Screening Core (HTS)
In response to the NIH Roadmap to Molecular Libraries and Imaging, we have fully established a
P50 High Throughput Core. The new Core represents a multi-departmental effort with fiscal support for
establishing the Core coming from P50 funds in combination with the Departments of Radiology, Molecular
Biology & Pharmacology, and Cell Biology as well as the Howard Hughes Medical Institute. The Dean of
Washington University School of Medicine as well as the Director of the Washington University Siteman
Cancer Center joined our effort with the commitment of additional funds for further development of the
resource. This Core serves as an outstanding on-going example of how our P50 program has
leveraged university resources and continues to stimulate interdisciplinary molecular imaging
activity throughout the WU campus. We have optimized several robotic protocols and logistical
requirements for our first projects in high throughput screening and exciting data are now coming on-line
during the summer of 2006. The Core allows for automated screening of cells cultured in 96 or 384 plate
formants and can be applied to multiple investigator-initiated molecular imaging applications throughout the
university community. The applications include:
1) small molecule screens applied to cells engineered by WU investigators for imaging and
therapeutic drug leads.
2) small molecule or peptide screens to identify enzyme inhibitors (directed against high priority
proteases, kinases, phosphatases, etc.), or modulators of protein-protein interactions.
3) genome-wide siRNA library screens against kinases, phosphatases and E3-ligases in human
cells targeting signal transduction, cell growth or cell death responses.
Our first projects are focused on use of the novel split luciferase complementation imaging platform
developed in the Molecular Reporter Core for analysis of protein-protein interactions using siRNA libraries
against all known expressed human kinases and phosphatases. Knock-down strategies are also being
used to explore fusion reporters for bioluminescence readouts of cell cycle regulation, the Notch signaling
pathway, and regulation of MDR1 gene expression and other related discovery projects. We will soon be
incorporating small molecule libraries from the Chemistry Core. These directly demonstrate the cycle of
synergies between the Molecular Reporter Core, the Chemistry Core, the HTS Core and ICMIC R&D
Projects.
A.3.III.高吞吐量筛选核心(HTS)
作为对NIH分子图书馆和成像路线图的响应,我们已经完全建立了
P50高吞吐量核心。新的核心代表了多部门的努力,并提供了财政支持
结合放射科、分子科建立P50经费核心
生物学和药理学、细胞生物学以及霍华德·休斯医学院。香港中文大学教务长
华盛顿大学医学院以及华盛顿大学西特曼主任
癌症中心加入了我们的努力,并承诺提供额外资金,以进一步发展
资源。这一核心是我们的P50计划如何具有
利用大学资源,继续促进跨学科分子成像
活动遍及整个吴校区。我们已经优化了几个机器人协议和后勤
我们在高通量筛选和令人兴奋的数据方面的首批项目需求现已上线
在2006年夏天。核心允许自动筛选培养在96或384平板中的细胞
共振峰,可应用于研究人员发起的多个分子成像应用
大学社区。这些应用程序包括:
1)小分子屏幕应用于吴研究员设计的细胞成像和
治疗性药物线索。
2)筛选小分子或多肽以识别酶抑制剂(针对高优先级
蛋白水解酶、激酶、磷酸酶等)或蛋白质-蛋白质相互作用的调节物。
3)全基因组siRNA文库筛选人类的激酶、磷酸酶和E3-连接酶
细胞以信号转导、细胞生长或细胞死亡反应为靶点。
我们的第一个项目集中在使用新的分裂荧光素酶互补成像平台
在分子报告核心中开发,用于使用siRNA文库分析蛋白质-蛋白质相互作用
对抗所有已知表达的人蛋白激酶和磷酸酶。击倒战略也在进行中
用于探索融合报告生物发光读数的细胞周期调控,Notch信号
途径、多药耐药基因表达的调控以及其他相关发现项目。我们很快就会
整合了化学核心的小分子文库。这些都直接证明了
分子报告核心、化学核心、高温超导核心与ICMIC研发的协同效应
项目。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
HELEN M PIWNICA-WORMS其他文献
HELEN M PIWNICA-WORMS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('HELEN M PIWNICA-WORMS', 18)}}的其他基金
Mechanisms of fasting-induced radioprotection of small intestinal epithelial cells
禁食诱导的小肠上皮细胞辐射防护机制
- 批准号:
10645872 - 财政年份:2023
- 资助金额:
$ 18.79万 - 项目类别:
Fasting Protects Small Intestinal Stem Cells from Lethal DNA Damage: Mechanistic Insight and Preclinical Translation
禁食可保护小肠干细胞免受致命性 DNA 损伤:机制洞察和临床前转化
- 批准号:
10090461 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
Fasting Protects Small Intestinal Stem Cells from Lethal DNA Damage: Mechanistic Insight and Preclinical Translation
禁食可保护小肠干细胞免受致命性 DNA 损伤:机制洞察和临床前转化
- 批准号:
9307224 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
8361353 - 财政年份:2011
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
8168703 - 财政年份:2010
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
7953916 - 财政年份:2009
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
7721480 - 财政年份:2008
- 资助金额:
$ 18.79万 - 项目类别:
Res Proj 2: Molecular Imaging Strategies to Study Cdc25A Regulation in vivo
Res Proj 2:研究 Cdc25A 体内调节的分子成像策略
- 批准号:
7287031 - 财政年份:2007
- 资助金额:
$ 18.79万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
7355307 - 财政年份:2006
- 资助金额:
$ 18.79万 - 项目类别:
相似海外基金
Establishment of a new biological assay using Hydra nematocyst deployment
利用水螅刺丝囊部署建立新的生物测定方法
- 批准号:
520728-2017 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
University Undergraduate Student Research Awards
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10368760 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10669539 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9570142 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9915803 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
COVID-19 Supplemental work: POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER).
COVID-19 补充工作:用于确定组织特异性吸收电离辐射剂量的护理点生物测定(生物剂量计)。
- 批准号:
10259999 - 财政年份:2017
- 资助金额:
$ 18.79万 - 项目类别:
Drug discovery based on a new biological assay system using Yeast knock-out strain collection
基于使用酵母敲除菌株收集的新生物测定系统的药物发现
- 批准号:
21580130 - 财政年份:2009
- 资助金额:
$ 18.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2005
- 资助金额:
$ 18.79万 - 项目类别:
Postdoctoral Fellowships
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2004
- 资助金额:
$ 18.79万 - 项目类别:
Postdoctoral Fellowships