CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
基本信息
- 批准号:8168703
- 负责人:
- 金额:$ 0.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-03-10 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:Amino AcidsChargeComputer Retrieval of Information on Scientific Projects DatabaseDataDissociationFourier transform ion cyclotron resonanceFundingGrantHumanInstitutionIonsIsomerismLinkMass Spectrum AnalysisMeasurementMethodsParentsPeptidesPhosphopeptidesPhosphoric AcidsPhosphorylated PeptidePhosphorylationPhosphorylation SitePhosphotransferasesResearchResearch PersonnelResourcesSourceUnited States National Institutes of Healthcheckpoint kinase 2mutantnanonovel
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
We employed data-dependent analysis of protein phosphorylation using rapid acquisition nano-LC-linear-quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry (nano-LC-FT-MS). The accurate m/z values of singly, doubly and triply-charged species calculated from the theoretical protonated masses of peptides phosphorylated at all Ser, Thr or Tyr residues of the human checkpoint 2 (Chk2) protein kinase were used for selected ion extraction and chromatographic analysis. Using a kinase-inactive Chk2 mutant as a control, accurate mass measurements from FT-MS and collision-induced dissociation spectra, 11 Chk2 auto-phosphorylation sites were assigned. Additionally, the presence of additional novel Chk2 phosphorylation sites in two unique peptides was deduced from accurate mass measurements. Selected ion chromatograms of all Chk2 phosphopeptides gave single peaks except in three cases in which two closely eluting species were observed. These pairs of phosphopeptides were determined to be positional isomers from MS/MS analysis. In this study, it was also found that ions due to the neutral loss of phosphoric acid from the parent peptide ion were not prominent in 18 of 36 MS/MS spectra of O-linked Chk2 phosphopeptides. Thus, accurate mass-driven analysis and rapid parallel MS/MS acquisition is a useful method for the discovery of new phosphorylation sites that is independent of the signature losses from phosphorylated amino acid residues.
这个子项目是众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('HELEN M PIWNICA-WORMS', 18)}}的其他基金
Mechanisms of fasting-induced radioprotection of small intestinal epithelial cells
禁食诱导的小肠上皮细胞辐射防护机制
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$ 0.97万 - 项目类别:
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10090461 - 财政年份:2017
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$ 0.97万 - 项目类别:
Fasting Protects Small Intestinal Stem Cells from Lethal DNA Damage: Mechanistic Insight and Preclinical Translation
禁食可保护小肠干细胞免受致命性 DNA 损伤:机制洞察和临床前转化
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9307224 - 财政年份:2017
- 资助金额:
$ 0.97万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
- 批准号:
8361353 - 财政年份:2011
- 资助金额:
$ 0.97万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
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7953916 - 财政年份:2009
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$ 0.97万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
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7721480 - 财政年份:2008
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$ 0.97万 - 项目类别:
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7287031 - 财政年份:2007
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$ 0.97万 - 项目类别:
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- 资助金额:
$ 0.97万 - 项目类别:
CHARACTERIZATION OF PROTEIN PHOSPHORYLATION OF HUMAN CHK2 PROTEIN KINASE
人 CHK2 蛋白激酶的蛋白磷酸化特征
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7355307 - 财政年份:2006
- 资助金额:
$ 0.97万 - 项目类别:
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