High Performance Gel and Blot Imager

高性能凝胶和印迹成像仪

• 批准号: 7212895
• 负责人: Bruce Robert Pitt
• 金额: $ 12.56万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212895
• 关键词: Biological; Biotechnology; Clinical Pharmacology; Color; DNA; Detection; Environmental Health; Fluorescence; Future; Gel; Image; Image Analysis; In Situ Hybridization; Individual; Institutes; Laboratories; Lasers; Medical; Medicine; Methods; Molecular; Molecular Medicine; Northern Blotting; Occupational Health; Performance; Polymerase Chain Reaction; Proteins; Public Health; Radioisotopes; Range; Regenerative Medicine; Research; Scanning; Site; Staining method; Stains; Students; Technology; Time; Training; Western Blotting; base; fundamental research; gene therapy; instrument; mRNA Differential Displays

DESCRIPTION (provided by applicant): This amended proposal is for a high performance gel and blot imager. The new instrument, General Electric-Amersham Biosciences Typhoon 9400, has three methods of detection (storage phosphor, fluorescence and chemiluminescence) and will serve as the only high sensitivity quantitative instrument for low abundance targets for multiple users in the Department of Environmental and Occupational Health (EOH), Center for Clinical Pharmacology, McGowan Institute Regenerative Medicine (MIRM) and Molecular Medicine Institute (MMI). The rationale for this request includes: a) EOH relocated to a new research (Cellomics) building at our Bridgeside Point site 1.5 miles from the main medical campus where the two current phosphoimagers are located; b) Center Clinical Pharmacology, MIRM and MMI are current occupants of the Cellomics Building and Biotechnology Center at the Bridgeside Point site and along with EOH have increased their emphasis on the utilization of molecular biological approaches that routinely require access to a broad based platform of high sensitivity and significant dynamic range; c) Typhoon 9400 will be a valuable platform for the training of pre- and postdoctoral students; d) subtle features of this instrument including 3 laser fluorescence should assure its utility in the future; and e) the short scanning time of the instrument enhances its multiuser capability by allowing more time consuming analysis to be done in individual laboratories. Applications with Typhoon 9400 include fluorescent DNA gel stain, 2-color fluorescent differential display, fluorescent protein gel stain, fluorescent multiplex PCR and Western blot analyses, imaging 2-D gels and chemiluminescence detection of proteins, phosphor imaging of radioisotopes for Northern blot and in situ hybridization. This enabling technology will advance fundamental research in environmental health sciences, regenerative medicine, clinical pharmacology and gene therapy and thereby facilitate broad based efforts in many aspects of medicine and public health.

描述（由申请人提供）：本修订提案针对高性能凝胶和印迹成像仪。新仪器通用电气-Amersham Biosciences Typhoon 9400有三种检测方法（存储磷光体，荧光和化学发光），并将作为环境和职业健康部（EOH），临床药理学中心，McGowan Institute Regenerative Medicine（MECHANISM）和Molecular Medicine Institute（MMI）。这一请求的理由包括：a）EOH搬迁到一个新的研究中心，（细胞组学）建设在我们的布里奇赛德点网站1.5英里的主要医学园区，其中两个目前的磷光成像仪所在地; B）临床药理学中心，目前，Mingdom和MMI是Bridgeside Point的Cellomics大楼和生物技术中心的住户，沿着EOH，他们越来越重视利用（c）Typhoon 9400将成为培训博士前和博士后学生的宝贵平台;（d）该仪器的细微特点，包括激光荧光，应确保其在未来的实用性;以及e）该仪器的短扫描时间通过允许在各个实验室中进行更耗时的分析来增强其多用户能力。Typhoon 9400的应用包括荧光DNA凝胶染色、双色荧光差异显示、荧光蛋白凝胶染色、荧光多重PCR和Western印迹分析、蛋白质的成像2-D凝胶和化学发光检测、放射性同位素的荧光成像用于北方印迹和原位杂交。这项使能技术将推进环境健康科学、再生医学、临床药理学和基因治疗的基础研究，从而促进医学和公共卫生许多方面的广泛努力。