Research 2 Laser Analytical Flow Cytometer

研究2 激光分析流式细胞仪

• 批准号: 6730918
• 负责人: Bruce Robert Pitt
• 金额: $ 10.6万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730918
• 关键词: apoptosis; biological signal transduction; biomedical equipment purchase; flow cytometry; microbiology; stem cells; transfection

DESCRIPTION (provided by applicant): This request is for funds to purchase an analytical bench top research two-laser flow cytometer. The new instrument, a BD FACSCalibur, will serve as the only analytical cytometer in Department of Environmental and Occupational Health (EOH) of the Graduate School Public Health (GSPH) and for users in nearby Magee Women's Research Institute (MWRI) who share common interests in environmental health science and additional work in maternal/child health. The rationale for this request includes: a) The Department of Environmental and Occupational Health (EOH) is in an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly increases our utilization of flow cytometry. This expansion coincides with the recent recruitment of Dr. Jerry Schatten and colleagues at our Magee Women's Research Institute (MWRI) thereby greatly further expanding our user base; b) EOH is in a newly renovated stand alone facility 0.2 to 0.4 miles from collaborating investigators (with the noted exception of much closer proximity to MWRI) requiring transit through unacclimatized outdoor routes minimizing the opportunity to share flow cytometers located in several other departments in our allied health system; c) Our major Flow Cytometry Core facility, directed by Dr. Donnenberg, relocated (September 2002) to the Hillman Cancer Center that is 1.5 miles away adding, to our need to establish an independent ability to perform analytical flow cytometry; d) The instrument is vital for training of pre- and postdoctoral students; and e) An analytical flow cytometer (e.g., FACSCalibur) will provide valuable quantitative approaches to support more complex research protocols in environmental health science with the aid of our colleagues on our advisory committee from Flow Cytometry Core (Dr. Donnenberg) and Center for Biological Imaging (Dr. Watkins). Applications will include multicolor immunophenotyping, physiological studies on transfected cells, signaling and apoptosis, stem cell biology and microbiology.

描述(申请人提供)：此申请是为购买分析型台式研究双激光流式细胞仪而提出的。这台新仪器是BD FACSCalibur，将作为研究生院公共卫生(GSPH)环境与职业卫生系(EOH)和附近Magee妇女研究所(MWRI)在环境健康科学和母婴健康方面有共同兴趣的用户的唯一分析细胞仪。提出这一要求的理由包括：a)环境和职业卫生部正处于广泛的招聘阶段，重点是当代环境健康科学，这大大增加了我们对流式细胞仪的使用。这一扩展与我们玛吉妇女研究所(MWRI)最近招募的曾傑瑞·沙滕博士和同事的招聘不谋而合，从而进一步扩大了我们的用户基础；b)EOH位于新装修的独立设施中，距离合作研究人员0.2至0.4英里(著名的例外是更接近MWRI)，需要通过未适应的户外路线过境，最大限度地减少共享位于我们联合卫生系统中其他几个部门的流量细胞仪的机会；C)我们的主要流式细胞仪核心设施在Donnenberg博士的指导下搬迁(2002年9月)到1.5英里外的Hillman癌症中心，从而增加了我们建立独立进行流式细胞术分析能力的必要性；d)该仪器对于博士后和博士后培训至关重要；e)分析型流式细胞仪(例如FACSCalibur)将在我们的咨询委员会同事的帮助下提供宝贵的定量方法，以支持环境健康科学中更复杂的研究方案。应用领域包括多色免疫表型、转基因细胞的生理学研究、信号和细胞凋亡、干细胞生物学和微生物学。