Research 2 Laser Analytical Flow Cytometer

研究2 激光分析流式细胞仪

• 批准号: 6730918
• 负责人: Bruce Robert Pitt
• 金额: $ 10.6万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730918
• 关键词: apoptosis; biological signal transduction; biomedical equipment purchase; flow cytometry; microbiology; stem cells; transfection

DESCRIPTION (provided by applicant): This request is for funds to purchase an analytical bench top research two-laser flow cytometer. The new instrument, a BD FACSCalibur, will serve as the only analytical cytometer in Department of Environmental and Occupational Health (EOH) of the Graduate School Public Health (GSPH) and for users in nearby Magee Women's Research Institute (MWRI) who share common interests in environmental health science and additional work in maternal/child health. The rationale for this request includes: a) The Department of Environmental and Occupational Health (EOH) is in an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly increases our utilization of flow cytometry. This expansion coincides with the recent recruitment of Dr. Jerry Schatten and colleagues at our Magee Women's Research Institute (MWRI) thereby greatly further expanding our user base; b) EOH is in a newly renovated stand alone facility 0.2 to 0.4 miles from collaborating investigators (with the noted exception of much closer proximity to MWRI) requiring transit through unacclimatized outdoor routes minimizing the opportunity to share flow cytometers located in several other departments in our allied health system; c) Our major Flow Cytometry Core facility, directed by Dr. Donnenberg, relocated (September 2002) to the Hillman Cancer Center that is 1.5 miles away adding, to our need to establish an independent ability to perform analytical flow cytometry; d) The instrument is vital for training of pre- and postdoctoral students; and e) An analytical flow cytometer (e.g., FACSCalibur) will provide valuable quantitative approaches to support more complex research protocols in environmental health science with the aid of our colleagues on our advisory committee from Flow Cytometry Core (Dr. Donnenberg) and Center for Biological Imaging (Dr. Watkins). Applications will include multicolor immunophenotyping, physiological studies on transfected cells, signaling and apoptosis, stem cell biology and microbiology.

描述（由申请人提供）：此请求是要资金购买分析台上研究两重点流式细胞仪。新仪器是BD FACSCALIBUR，将成为研究生院公共卫生（GSPH）环境与职业健康部（EOH）的唯一分析细胞仪，以及附近的Magee妇女研究所（MWRI）的用户，他们在环境健康科学方面具有共同利益，并在产妇/儿童健康方面具有其他工作。该请求的基本原理包括：a）环境和职业健康部（EOH）处于广泛的招聘阶段，重点是当代环境健康科学，这大大增加了我们对流式细胞仪的利用。这一扩展与我们的Magee妇女研究所（MWRI）最近招募Jerry Schatten博士及其同事的招募相吻合，从而大大扩大了我们的用户群。 b）EOH正处于一个新近装修的独立设施中，距离合作调查人员（指出的距离MWRI更近外的距离）为0.2至0.4英里，需要通过未策略的户外路线进行过境，从而最小化了共享我们同类卫生系统中其他几个部门中的流式细胞仪的机会， c）由Donnenberg博士执导的主要流式细胞仪核心设施（2002年9月）迁至Hillman Cancer Center，该中心增加了1.5英里，我们需要建立独立的能力来执行分析流式细胞仪； d）该乐器对于培训学前和博士后学生至关重要； e）分析流式细胞仪（例如FACScalibur）将提供有价值的定量方法，以支持我们的同事在流式细胞仪核心（Donnenberg博士）和生物学成像中心（沃特金斯博士）的咨询委员会的咨询委员会上，支持环境健康科学中更复杂的研究方案。应用将包括多色免疫表型，转染细胞的生理研究，信号传导和凋亡，干细胞生物学和微生物学。