Research 2 Laser Analytical Flow Cytometer

研究2 激光分析流式细胞仪

• 批准号: 6730918
• 负责人: Bruce Robert Pitt
• 金额: $ 10.6万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730918
• 关键词: apoptosis; biological signal transduction; biomedical equipment purchase; flow cytometry; microbiology; stem cells; transfection

DESCRIPTION (provided by applicant): This request is for funds to purchase an analytical bench top research two-laser flow cytometer. The new instrument, a BD FACSCalibur, will serve as the only analytical cytometer in Department of Environmental and Occupational Health (EOH) of the Graduate School Public Health (GSPH) and for users in nearby Magee Women's Research Institute (MWRI) who share common interests in environmental health science and additional work in maternal/child health. The rationale for this request includes: a) The Department of Environmental and Occupational Health (EOH) is in an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly increases our utilization of flow cytometry. This expansion coincides with the recent recruitment of Dr. Jerry Schatten and colleagues at our Magee Women's Research Institute (MWRI) thereby greatly further expanding our user base; b) EOH is in a newly renovated stand alone facility 0.2 to 0.4 miles from collaborating investigators (with the noted exception of much closer proximity to MWRI) requiring transit through unacclimatized outdoor routes minimizing the opportunity to share flow cytometers located in several other departments in our allied health system; c) Our major Flow Cytometry Core facility, directed by Dr. Donnenberg, relocated (September 2002) to the Hillman Cancer Center that is 1.5 miles away adding, to our need to establish an independent ability to perform analytical flow cytometry; d) The instrument is vital for training of pre- and postdoctoral students; and e) An analytical flow cytometer (e.g., FACSCalibur) will provide valuable quantitative approaches to support more complex research protocols in environmental health science with the aid of our colleagues on our advisory committee from Flow Cytometry Core (Dr. Donnenberg) and Center for Biological Imaging (Dr. Watkins). Applications will include multicolor immunophenotyping, physiological studies on transfected cells, signaling and apoptosis, stem cell biology and microbiology.

描述（由申请人提供）：本申请是为购买分析台式研究双激光流式细胞仪提供资金。新仪器是BD FACSCalibur，将作为研究生院公共卫生（GSPH）环境和职业卫生（EOH）系以及附近Magee妇女研究所（MWRI）的用户的唯一分析细胞仪，他们在环境卫生科学和孕产妇/儿童健康方面有着共同的兴趣。这一要求的理由包括：a）环境和职业卫生部（EOH）正处于广泛的招聘阶段，重点是当代环境卫生科学，这大大增加了我们对流式细胞术的利用。这一扩张与我们的Magee妇女研究所（MWRI）最近招聘Jerry Schatten博士和同事相吻合，从而大大进一步扩大了我们的用户群; B）EOH位于一个新装修的独立机构中，距离合作研究者0.2至0.4英里（除了与MWRI更近的地方）要求通过不适应环境的户外路线运输，从而最大限度地减少了在我们的联合卫生系统中共享其他几个部门的流式细胞仪的机会; c）由Donnenberg博士领导的我们的主要流式细胞仪核心设施搬迁（2002年9月）到1.5英里外的希尔曼癌症中心，增加了我们建立独立的流式细胞术分析能力的需要; d）该仪器对于博士前和博士后学生的培训至关重要;以及FACSCalibur）将提供有价值的定量方法，以支持环境健康科学中更复杂的研究方案，并得到我们咨询委员会中来自流式细胞术核心（Donnenberg博士）和生物成像中心（Watkins博士）的同事的帮助。应用将包括免疫表型分析，转染细胞的生理学研究，信号传导和凋亡，干细胞生物学和微生物学。