Bioplex 200 System Package

Bioplex 200 系统包

• 批准号: 7587178
• 负责人: Bruce Robert Pitt
• 金额: $ 10.37万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-21 至 2010-05-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7587178
• 关键词: Animal Model; Animals; Antibodies; Antigens; Biological; Biological Assay; Cardiovascular system; Cell Culture Techniques; Clinical Pharmacology; Code; Color; Computer software; Data Analyses; Environmental Health; Enzyme Immunoassay; Experimental Models; Funding; Future; Hospitals; Human; Individual; Laboratories; Ligands; Magnetism; Mammalian Cell; Measurement; Modeling; Molecular; Mus; Nature; Nervous System Physiology; Neuraxis; Nucleic Acids; Occupational Health; Oligonucleotides; Peptides; Pharmacogenomics; Phase; Polystyrenes; Proteins; Quantitative Trait Loci; Reader; Reagent; Research; Research Personnel; Scanning; Serum; Signal Transduction; Site; Students; Surface; Suspension substance; Suspensions; System; Systems Analysis; Technology; Time; Training; University of Pittsburgh Cancer Institute; enzyme substrate; gene environment interaction; high throughput screening; human subject; instrument; public health relevance; receptor; research study; respiratory; synthetic polymer Bioplex; transcription factor

DESCRIPTION (provided by applicant): We are requesting funds for a Bio-Rad Laboratories Bio-Plex Workstation and Software (Suspension Array) system to provide a multiplex system to facilitate simultaneous analysis of up to 100 different biomolecules (proteins, peptides or nucleic acids) in a single microplate reader. The multiplex platform integrates xMAP(R) technology and Luminex200" from Luminex Corporation to enable discrete bioassays on the surface of color coded beads (either polystyrene or magnetic). Reagents may include antigens, antibodies, oligonucleotides, enzyme substrates or receptors and thus can provide quantitative information of multiple analytes in small volumes for experimental (animal and human) or cell culture analyte profiling, signal transduction profiling and transcriptional profiling. The multiplex analysis system (Bio-Plex) will be used to support a group of 13 investigators in the Department of Environmental and Occupational Health (EOH). The rationale for this request includes: a) EOH is an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly has increased the utilization of molecular biological approaches in experimental models in genetically modified mice including quantitative trait locus approaches towards gene-environment interactions, mammalian cell culture and human subjects; b) EOH recently relocated to a new contemporary research building (Thermo-Fisher Building) in the Bridgeside Point Site (100 Technology Drive) that is >3.0 miles from the major cluster of Bio-Plex instruments (n=5) at University Pittsburgh Cancer Institute; and our current collaborators at Childrens Hospital of Pittsburgh; c) there is only one Bio-Plex instrument nearby (Clinical Pharmacology) and this is dedicated to a large pharmacogenomic effort and was not configured to accommodate the entire needs of our department; d) Bio-Plex will be a valuable platform for the training of pre- and postdoctoral students in environmental health sciences; e) the versatility (proteins and nucleic acids) of Bio-Plex will assure that it conforms to predicted future needs of serving as a critical interface with other discrete high throughput screening; f) the short scanning time of the instrument enhances its multiuser capability by allowing more time consuming analysis to be done in individual laboratories and the multiuser software components will facilitate off site data analysis further simplifying multiuser access. The Bio-Plex platform is capable of assaying a wide range of biomolecules including proteins and nucleic acids and as such applications can include immunoassays, enzyme assays, receptor ligand interactions and signal transduction assays as well as transcription factor profiling. We anticipate extensive initial use in animal model serum and cell culture model analyte profiling and expanding use in signal transduction network profiling and transcription factor profiling. PUBLIC HEALTH RELEVANCE: The acquisition of Bio-Plex for 13 sponsored investigators in EOH will greatly facilitate existing funded research in environmental health related to respiratory, cardiovascular and central nervous system function. The ability to make multiple measurements of protein and nucleic acids in a small volume will greatly enhance the integrated nature of virtually all experiments and allow for considerably more meaningful results to emerge.

描述（由申请人提供）：我们要求为Bio-Rad Laboratories Bio-Plex工作站和软件（悬架阵列）系统提供资金，以提供一个多重系统，以促进单个微型层读取器中多达100种不同的生物分子（蛋白质，肽或核酸）同时分析。多重平台从Luminex Corporation集成了XMAP（R）技术和Luminex200”，以在颜色编码的珠子（聚苯乙烯或磁性）的表面上启用离散的生物测定。试剂可能包括抗原，抗体，抗体，寡核苷酸，因此可以提供固定培养物或量化（少量培养物）的多数分析（少量分析）。分析，信号转导分析和转录分析。定量性状基因座用于基因环境相互作用，哺乳动物细胞培养和人类受试者的方法； b）EOH最近搬到了Bridgeside Point Site（100 Technology Drive）的新的当代研究大楼（Thermo-Fisher大楼），距离匹兹堡大学匹兹堡癌症研究所的Bio-Plex仪器（N = 5）的主要群体> 3.0英里；以及我们目前在匹兹堡儿童医院的合作者； c）附近只有一种生物质仪器（临床药理学），这是针对大型药物基因组学的努力，并且没有配置以适应我们部门的全部需求； d）生物质量将成为培训环境健康科学前和博士后学生的宝贵平台； e）生物质量的多功能性（蛋白质和核酸）将确保它符合预测将来作为与其他离散高吞吐量筛选的关键接口的未来需求； f）仪器的短扫描时间通过允许在单个实验室中进行更多耗时的分析来增强其多源功能，并且多用户软件组件将有助于偏离站点数据分析，从而进一步简化了多用户访问。 Bio-plex平台能够分析包括蛋白质和核酸在内的广泛的生物分子，因此应用可以包括免疫测定，酶测定，受体配体相互作用和信号转导测定法以及转录因子分析。我们预计在动物模型血清和细胞培养模型分析物分析以及在信号转导网络分析和转录因子分析中的扩展中有广泛的初始使用。 公共卫生相关性：在EOH中为13位赞助的研究人员收购Bio-Plex将极大地促进与呼吸道，心血管和中枢神经系统功能有关的环境健康研究的现有资助研究。在较小体积中对蛋白质和核酸进行多次测量的能力将大大增强几乎所有实验的综合性质，并允许出现更有意义的结果。