Bioplex 200 System Package

Bioplex 200 系统包

• 批准号: 7587178
• 负责人: Bruce Robert Pitt
• 金额: $ 10.37万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-21 至 2010-05-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7587178
• 关键词: Animal Model; Animals; Antibodies; Antigens; Biological; Biological Assay; Cardiovascular system; Cell Culture Techniques; Clinical Pharmacology; Code; Color; Computer software; Data Analyses; Environmental Health; Enzyme Immunoassay; Experimental Models; Funding; Future; Hospitals; Human; Individual; Laboratories; Ligands; Magnetism; Mammalian Cell; Measurement; Modeling; Molecular; Mus; Nature; Nervous System Physiology; Neuraxis; Nucleic Acids; Occupational Health; Oligonucleotides; Peptides; Pharmacogenomics; Phase; Polystyrenes; Proteins; Quantitative Trait Loci; Reader; Reagent; Research; Research Personnel; Scanning; Serum; Signal Transduction; Site; Students; Surface; Suspension substance; Suspensions; System; Systems Analysis; Technology; Time; Training; University of Pittsburgh Cancer Institute; enzyme substrate; gene environment interaction; high throughput screening; human subject; instrument; public health relevance; receptor; research study; respiratory; synthetic polymer Bioplex; transcription factor

DESCRIPTION (provided by applicant): We are requesting funds for a Bio-Rad Laboratories Bio-Plex Workstation and Software (Suspension Array) system to provide a multiplex system to facilitate simultaneous analysis of up to 100 different biomolecules (proteins, peptides or nucleic acids) in a single microplate reader. The multiplex platform integrates xMAP(R) technology and Luminex200" from Luminex Corporation to enable discrete bioassays on the surface of color coded beads (either polystyrene or magnetic). Reagents may include antigens, antibodies, oligonucleotides, enzyme substrates or receptors and thus can provide quantitative information of multiple analytes in small volumes for experimental (animal and human) or cell culture analyte profiling, signal transduction profiling and transcriptional profiling. The multiplex analysis system (Bio-Plex) will be used to support a group of 13 investigators in the Department of Environmental and Occupational Health (EOH). The rationale for this request includes: a) EOH is an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly has increased the utilization of molecular biological approaches in experimental models in genetically modified mice including quantitative trait locus approaches towards gene-environment interactions, mammalian cell culture and human subjects; b) EOH recently relocated to a new contemporary research building (Thermo-Fisher Building) in the Bridgeside Point Site (100 Technology Drive) that is >3.0 miles from the major cluster of Bio-Plex instruments (n=5) at University Pittsburgh Cancer Institute; and our current collaborators at Childrens Hospital of Pittsburgh; c) there is only one Bio-Plex instrument nearby (Clinical Pharmacology) and this is dedicated to a large pharmacogenomic effort and was not configured to accommodate the entire needs of our department; d) Bio-Plex will be a valuable platform for the training of pre- and postdoctoral students in environmental health sciences; e) the versatility (proteins and nucleic acids) of Bio-Plex will assure that it conforms to predicted future needs of serving as a critical interface with other discrete high throughput screening; f) the short scanning time of the instrument enhances its multiuser capability by allowing more time consuming analysis to be done in individual laboratories and the multiuser software components will facilitate off site data analysis further simplifying multiuser access. The Bio-Plex platform is capable of assaying a wide range of biomolecules including proteins and nucleic acids and as such applications can include immunoassays, enzyme assays, receptor ligand interactions and signal transduction assays as well as transcription factor profiling. We anticipate extensive initial use in animal model serum and cell culture model analyte profiling and expanding use in signal transduction network profiling and transcription factor profiling. PUBLIC HEALTH RELEVANCE: The acquisition of Bio-Plex for 13 sponsored investigators in EOH will greatly facilitate existing funded research in environmental health related to respiratory, cardiovascular and central nervous system function. The ability to make multiple measurements of protein and nucleic acids in a small volume will greatly enhance the integrated nature of virtually all experiments and allow for considerably more meaningful results to emerge.

描述（由申请人提供）：我们正在为 Bio-Rad 实验室 Bio-Plex 工作站和软件（悬浮阵列）系统申请资金，以提供多重系统，以便在单个酶标仪中同时分析多达 100 种不同的生物分子（蛋白质、肽或核酸）。该多重平台集成了 xMAP(R) 技术和 Luminex Corporation 的 Luminex200"，可在颜色编码珠（聚苯乙烯或磁性）表面上进行离散生物测定。试剂可能包括抗原、抗体、寡核苷酸、酶底物或受体，因此可以为实验（动物和人类）或细胞培养分析物提供小体积多种分析物的定量信息 分析、信号转导分析和转录分析。多重分析系统 (Bio-Plex) 将用于支持环境和职业健康部 (EOH) 的 13 名研究人员组成的小组。这一请求的基本原理包括： a) EOH 是一个广泛的招募阶段，重点是当代环境健康科学，大大增加了分子生物学方法在转基因小鼠实验模型中的利用率，包括 针对基因-环境相互作用、哺乳动物细胞培养和人类受试者的数量性状基因座方法； b) EOH 最近搬迁至 Bridgeside Point 站点（100 Technology Drive）的一座新的当代研究大楼（Thermo-Fisher 大楼），距离匹兹堡大学癌症研究所的主要 Bio-Plex 仪器集群（n=5）>3.0 英里；以及我们目前在儿童医院的合作者 匹兹堡； c) 附近只有一台 Bio-Plex 仪器（临床药理学），该仪器专用于大型药物基因组学研究，其配置无法满足我们部门的全部需求； d) Bio-Plex 将成为环境健康科学博士前和博士后培训的宝贵平台； e) Bio-Plex 的多功能性（蛋白质和核酸）将确保 它符合作为其他离散高通量筛选的关键接口的预测未来需求； f) 仪器的扫描时间短，允许在各个实验室进行更耗时的分析，从而增强了其多用户能力，并且多用户软件组件将促进场外数据分析，进一步简化多用户访问。 Bio-Plex 平台能够检测多种生物分子，包括蛋白质和 核酸及其应用可包括免疫测定、酶测定、受体配体相互作用和信号转导测定以及转录因子分析。我们预计最初将广泛用于动物模型血清和细胞培养模型分析物分析，并扩大在信号转导网络分析和转录因子分析中的使用。 公共卫生相关性：为 EOH 的 13 名赞助研究人员收购 Bio-Plex 将极大地促进 现有资助的与呼吸、心血管和中枢神经系统功能相关的环境健康研究。在小体积内对蛋白质和核酸进行多次测量的能力将大大增强几乎所有实验的集成性质，并允许出现更有意义的结果。