High Performance Research Flow Cytometer

高性能研究流式细胞仪

• 批准号: 7217177
• 负责人: David H Sherr
• 金额: $ 28.57万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217177
• 关键词: Performance; Research; Flow; Cytometer

DESCRIPTION (provided by applicant): Fifty four NIH-funded investigators (13 major users, 41 minor users) at the Boston University School of Medicine/Boston Medical Center Campus (BUSM/BMC) require flow cytometry for their NIH-funded studies. These investigators represent most of the departments and divisions within the medical center including, but not limited to the Immunology Training Program, the Cancer Center, the Pulmonary Division, the Amyloid Treatment and Research Program, the Women's Health Interdisciplinary Research Center, and 12 departments in the Schools of Medicine, Public Health, and Dentistry. However, relatively limited flow cytometry resources are available to these investigators. There are three 1980s era BD FACScans and one operator-assisted MoFlo cell sorter on the BUSM/BMC campus. The ageing FACScans are significantly outdated and are insufficient for many of the more advanced studies which exploit emerging technologies and staining reagents. All the instruments are at or beyond full capacity even as the demand for this technology grows. BU investigators have little if any access to the more sophisticated equipment available at other research facilities in the Boston area. Research that is highly relevant to public health (e.g., treatments for cancer, autoimmunity, and infectious disease; environmental causes of cancer) is severely compromised by this lack of appropriate instrumentation. Funds are requested for the purchase of a Becton Dickinson LSR II high performance flow cytometer with solid state lasers for 11 color (upgradable to 18 color) detection, automatic compensation, a high sampling rate, advanced signal detection and data processing, and a 96-well high throughput sample loader. The LSR II would dramatically improve cell analysis capabilities and would draw other members of the BUSM/BMC community into collaborative studies requiring this essential technology. Specific applications would include: detection of rare lymphocyte subsets and accurate assessment of kinase activity, cell activation, proliferation, apoptosis, cytokine production, and Ca2+ flux within specific cell subpopulations. Significant expertise is present at BU to perform these studies. In conjunction with emerging technologies, the LSR II would dramatically enhance the efficiency, quality, and capability of data acquisition for investigators at this institution while reducing costs. In recognition of these goals, BUSM/BMC is committed to substantial ongoing financial support of this instrumentation and its applications.

描述（由申请人提供）：波士顿大学医学院/波士顿医学中心校区（BUSM/BMC）的54名NIH资助的研究者（13名主要用户，41名次要用户）需要流式细胞术进行NIH资助的研究。这些研究人员代表了医学中心内的大多数部门和部门，包括但不限于免疫学培训计划，癌症中心，肺部部门，淀粉样蛋白治疗和研究计划，妇女健康跨学科研究中心以及医学，公共卫生和牙科学院的12个部门。 然而，相对有限的流式细胞术资源可用于这些研究者。在BUSM/BMC校园里有三台20世纪80年代的BD FACScan和一台操作员辅助的MoFlo细胞分选机。老化的FACScans明显过时，不足以用于许多利用新兴技术和染色试剂的更先进的研究。即使对这项技术的需求不断增长，所有的仪器都达到或超过了最大能力。波士顿大学的研究人员几乎没有机会接触到波士顿地区其他研究机构提供的更先进的设备。与公共卫生高度相关的研究（例如，癌症、自身免疫和传染病的治疗;癌症的环境原因）由于缺乏适当的仪器而严重受损。请拨资金用于购买一台Becton Dickinson LSR II型高性能流式细胞仪，该流式细胞仪配有固态激光器，可进行11色（可转换为18色）检测、自动补偿、高采样率、先进的信号检测和数据处理，以及一台96孔高通量样品装载机。LSR II将极大地提高细胞分析能力，并将吸引BUSM/BMC社区的其他成员参与需要这项关键技术的合作研究。具体应用包括：检测罕见的淋巴细胞亚群和准确评估激酶活性、细胞活化、增殖、凋亡、细胞因子产生和特定细胞亚群内的Ca 2+通量。BU拥有进行这些研究的重要专业知识。结合新兴技术，LSR II将大大提高该机构调查人员数据采集的效率、质量和能力，同时降低成本。认识到这些目标，BUSM/BMC致力于为该仪器及其应用提供大量持续的财政支持。