Upgrade of Femtosecond Laser Sources for Optical Workstation

光学工作站飞秒激光源升级

• 批准号: 7226460
• 负责人: Kevin William Eliceiri
• 金额: $ 19万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7226460
• 关键词: Ablation; Cell division; Cells; Computers; Depth; Disease; Equipment; Experimental Designs; Fostering; Health; Image; Intervention; Laboratories; Lasers; Life; Localized; Microscopic; Microsurgery; Optics; Pattern; Photobleaching; Physiologic pulse; Pliability; Process; Public Health; Pulse taking; Purpose; Range; Relative (related person); Role; Sapphire; Scanning; Signal Transduction; Source; Specimen; System; Techniques; Tissues; Universities; Wisconsin; concept; design; fluorophore; improved; in vivo; insight; instrumentation; malignant breast neoplasm; optical traps; photolysis; tool; tumor progression

DESCRIPTION (provided by applicant): Experimental laser microbeam techniques have become established tools for studying living specimens. A steerable, focused laser beam may be used for a variety of experimental manipulations such as laser microsurgery, optical trapping, localized photolysis of caged bioactive probes, and patterned photobleaching. Typically, purpose- designed experimental systems have been constructed for each of these applications. In order to assess the consequences of such experimental optical interventions, long-term, microscopic observation of the specimen is often required. Multiphoton excitation, because of its ability to obtain high-contrast images from deep within a specimen with minimal phototoxic effects, is a preferred technique for in vivo imaging. We have developed an Optical Workstation (OWS) that combines the functionality of an experimental optical microbeam apparatus with a sensitive multiphoton imaging system designed for use with living specimens. The system was developed at the Laboratory for Optical and Computational Instrumentation (LOCI) at the University of Wisconsin, Madison. The integrated optical workstation concept offers advantages in terms of flexibility and versatility relative to systems implemented with separate imaging and experimental components. This application is for equipment to enhance the capabilities of the OWS and to replace a defunct component. Two femtosecond laser sources are requested. Both sources will replace/upgrade existing lasers: an older Ti:Sapphire laser and a now defunct 1047 laser. The requested lasers are: a Mai Tai computer tunable Ti:Sapphire laser with a tuning range of 700-1000nm and a 1030nm modelocked t-Pulse 20 laser. The lasers will operate with the laser ablation and dual scanning control systems currently functioning on the OWS. This will both add new features and restore lost functionality, such as dual fluorophore excitation together with simultaneous optical trapping and uncaging. The upgraded OWS will have an expanded role on the UW campus by better serving current projects through improved functionality, and by fostering new projects that can take advantage of its expanded wavelength and manipulation capabilities. Relevance to Public Health: The ability to image non-invasively into live cells and tissue is of great potential benefit to public health as it can provide new insight into key disease processes such as cancer progression. The requested laser sources will benefit several health related imaging projects ranging from studying cell division to understanding the role of signaling in breast cancer.

描述（由申请人提供）：实验激光微束技术已成为研究活体标本的既定工具。可操纵的聚焦激光束可用于各种实验操作，例如激光显微手术、光学捕获、笼状生物活性探针的局部光解和图案化光漂白。通常，针对这些应用中的每一个构建了专门设计的实验系统。为了评估这种实验性光学干预的后果，通常需要对样品进行长期的显微镜观察。多光子激发，因为它的能力，以获得高对比度的图像，从一个标本内的最小的光毒性效应，是一个优选的技术，在体内成像。我们开发了一种光学工作站（OWS），它将实验光学微束装置的功能与专为活体标本使用而设计的灵敏多光子成像系统相结合。该系统是在位于麦迪逊的威斯康星州大学的光学和计算仪器实验室（LOCI）开发的。集成光学工作站的概念提供了相对于单独的成像和实验组件实现的系统的灵活性和多功能性方面的优势。此应用程序用于设备，以增强OWS的功能，并更换已失效的组件。需要两个飞秒激光源。这两种光源将取代/升级现有的激光器：旧的钛：蓝宝石激光器和现已停产的1047激光器。要求的激光器是：Mai Tai计算机可调谐Ti：Sapphire激光器，调谐范围为700- 1000 nm，以及1030 nm锁模t-Pulse 20激光器。激光器将与目前在OWS上运行的激光烧蚀和双扫描控制系统一起运行。这将增加新的功能并恢复丢失的功能，例如双荧光团激发以及同时的光学捕获和释放。升级后的OWS将在UW校园中发挥更大的作用，通过改进功能更好地服务于当前项目，并通过培养可以利用其扩展波长和操作能力的新项目。与公共卫生的相关性：对活细胞和组织进行非侵入性成像的能力对公共卫生具有巨大的潜在益处，因为它可以为癌症进展等关键疾病过程提供新的见解。所要求的激光源将有利于几个健康相关的成像项目，从研究细胞分裂到了解信号在乳腺癌中的作用。