SPECIFIC CHEMICAL INTERACTIONS IN MATRIX-INCORPORATED INSULIN STRUCTURES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。基质辅助激光解吸电离质谱仪(MALDI-MS)已成为生物大分子研究领域的一项强有力的技术。基质是一种有机小分子，在质谱学方法中起着关键作用。MALDI方法是经验性发展起来的，其机理尚不完全清楚。这个项目的动机是了解基质分子是如何工作的，或者它是如何与蛋白质相互作用的。这项工作的目的是找到蛋白质和基质分子之间的特定相互作用，因此它将有助于解释蛋白质阳离子的形成和质子转移是如何发生的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been a powerful technique in the bio-macromolecules field. The matrix, a small organic molecule, plays a key role in the mass spectral method. The MALDI method has been developed empirically and its mechanism is not yet fully understood. The motivation of this project is to understand how the matrix molecule works or how it interacts with the protein. The aim of this work is to find specific interactions between the protein and the matrix molecule, so it will help explain the formation of a protein cation and how the proton transfer occurs.