HIGH RESOLUTION STRUCTURES BY MINIMIZING RADIATION EFFECTS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。蛋白质晶体的辐射损伤，特别是因为数据收集在第三代同步加速器设施，是一个问题，越来越受到关注。超高分辨率结构是人们渴望获得的。然而，超高分辨率结构对辐射损伤最敏感并且受辐射损伤的影响最大。我们应用了我们为时间依赖结构观测开发的新策略，以消除或最小化辐射影响，从而从标准数据集产生真实的高分辨率结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Radiation damage on protein crystals, especially since data is collected at third generation synchrotron facilities, is an issue and is getting more and more attention. Ultra high resolution structures are what people aspire to obtain. However, ultra high resolution structures are most sensitive to and affected by radiation damage. We applied the new strategy we developed for time-dependent structure observations to remove or minimize radiation effects and thus yield the real high resolution structure from a standard dataset.