HIGH RESOLUTION STRUCTURES BY MINIMIZING RADIATION EFFECTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。蛋白质晶体的辐射损伤，特别是由于数据是在第三代同步加速器设备上收集的，是一个问题，并正受到越来越多的关注。超高分辨率结构是人们向往的目标。然而，超高分辨率结构对辐射损伤最敏感，受辐射损伤的影响最大。我们应用了我们为随时间变化的结构观测开发的新策略，以消除或最小化辐射影响，从而从标准数据集中产生真正的高分辨率结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Radiation damage on protein crystals, especially since data is collected at third generation synchrotron facilities, is an issue and is getting more and more attention. Ultra high resolution structures are what people aspire to obtain. However, ultra high resolution structures are most sensitive to and affected by radiation damage. We applied the new strategy we developed for time-dependent structure observations to remove or minimize radiation effects and thus yield the real high resolution structure from a standard dataset.