IMPLICATIONS OF DISULFIDES BREAKING SEQUENCE IN REDUCTIVE UNFOLDING PATHWAYS

二硫化物断裂序列对还原性展开路径的影响

• 批准号: 7369518
• 负责人: JUN WANG
• 金额: $ 0.04万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369518
• 关键词: IMPLICATIONS; DISULFIDES; BREAKING; SEQUENCE; REDUCTIVE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sequence of the breakage of disulfide bonds due to x-ray irradiation was observed by time-dependent crystallographic studies of proteins using the strategy we developed for time-dependent structure observations on the atomic scale in a protein crystal for standard crystallography experiments. These observed sequences are related to the reductive unfolding pathways in proteins. ¿he current study focuses on bovine pancreatic ribonuclease A, where the observed breaking sequence is being compared to results by other established methods.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。使用我们为标准晶体学实验在蛋白质晶体的原子尺度上进行时间依赖性结构观察而开发的策略，通过蛋白质的时间依赖性晶体学研究观察了由于 X 射线照射而导致的二硫键断裂的序列。这些观察到的序列与蛋白质中的还原性展开途径有关。目前的研究重点是牛胰腺核糖核酸酶 A，将观察到的断裂序列与其他既定方法的结果进行比较。