IMPLICATIONS OF DISULFIDES BREAKING SEQUENCE IN REDUCTIVE UNFOLDING PATHWAYS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sequence of the breakage of disulfide bonds due to x-ray irradiation was observed by time-dependent crystallographic studies of proteins using the strategy we developed for time-dependent structure observations on the atomic scale in a protein crystal for standard crystallography experiments. These observed sequences are related to the reductive unfolding pathways in proteins. ¿he current study focuses on bovine pancreatic ribonuclease A, where the observed breaking sequence is being compared to results by other established methods.