IMPLICATIONS OF DISULFIDES BREAKING SEQUENCE IN REDUCTIVE UNFOLDING PATHWAYS

二硫化物断裂序列对还原性展开路径的影响

• 批准号: 7369518
• 负责人: JUN WANG
• 金额: $ 0.04万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369518
• 关键词: IMPLICATIONS; DISULFIDES; BREAKING; SEQUENCE; REDUCTIVE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sequence of the breakage of disulfide bonds due to x-ray irradiation was observed by time-dependent crystallographic studies of proteins using the strategy we developed for time-dependent structure observations on the atomic scale in a protein crystal for standard crystallography experiments. These observed sequences are related to the reductive unfolding pathways in proteins. ¿he current study focuses on bovine pancreatic ribonuclease A, where the observed breaking sequence is being compared to results by other established methods.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。利用我们为标准结晶学实验开发的在原子尺度上观察蛋白质晶体中随时间变化的结构的策略，通过对蛋白质的随时间变化的结晶学研究，观察到了由于X射线照射而导致的二硫键断裂的顺序。这些观察到的序列与蛋白质中的还原展开途径有关。目前的研究集中在牛胰腺核糖核酸酶A上，观察到的断裂序列正在与其他已建立的方法的结果进行比较。