IMPLICATIONS OF DISULFIDES BREAKING SEQUENCE IN REDUCTIVE UNFOLDING PATHWAYS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 由于X射线照射的二硫键断裂的顺序观察到的时间依赖性的蛋白质晶体学研究，使用的策略，我们开发的时间依赖性的结构观察在原子尺度上的蛋白质晶体的标准晶体学实验。 这些观察到的序列与蛋白质中的还原性去折叠途径有关。 目前的研究集中在牛胰腺核糖核酸酶A，其中观察到的断裂序列正在与其他已建立的方法的结果进行比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sequence of the breakage of disulfide bonds due to x-ray irradiation was observed by time-dependent crystallographic studies of proteins using the strategy we developed for time-dependent structure observations on the atomic scale in a protein crystal for standard crystallography experiments. These observed sequences are related to the reductive unfolding pathways in proteins. he current study focuses on bovine pancreatic ribonuclease A, where the observed breaking sequence is being compared to results by other established methods.