IMPLICATIONS OF DISULFIDES BREAKING SEQUENCE IN REDUCTIVE UNFOLDING PATHWAYS

二硫化物断裂序列对还原性展开路径的影响

• 批准号: 7721227
• 负责人: JUN WANG
• 金额: $ 0.2万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721227
• 关键词: Bos taurus; Cattle; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Disulfides; Funding; Grant; Institution; Methods; Pancreatic ribonuclease; Pathway interactions; Proteins; Research; Research Personnel; Resources; Source; Standards of Weights and Measures; Structure; Time; United States National Institutes of Health; disulfide bond; irradiation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sequence of the breakage of disulfide bonds due to x-ray irradiation was observed by time-dependent crystallographic studies of proteins using the strategy we developed for time-dependent structure observations on the atomic scale in a protein crystal for standard crystallography experiments. These observed sequences are related to the reductive unfolding pathways in proteins. he current study focuses on bovine pancreatic ribonuclease A, where the observed breaking sequence is being compared to results by other established methods.

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