Biocompatibility and Function in a Troponin-T Biosensor

肌钙蛋白-T 生物传感器的生物相容性和功能

基本信息

批准号：
7256945
负责人：
LISA Wayland ALTSCHUL
金额：
$ 3.53万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-07-03 至 2009-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7256945
关键词：
Acute Acute myocardial infarction Address Adsorption Animals Antibodies Antigens Arginine Aspartic Acid Binding Biocompatible Biological Biosensor Blood Blood Circulation Blood Vessels Complex Dependency Detection Development Diffusion Electrodes Elements Energy Transfer Engineering Erythrocytes Fiber Fiber Optics Fibrinogen Fluorescence Gel Glutamic Acid Goals Grant Hour IACUC Immobilization In Vitro Individual Infarction Internal jugular vein structure Knowledge Label Laboratories Lead Left Length Measurable Mentors Methods Modification Molecular Conformation Myocardial Infarction Myocardium Names National Research Service Awards New Zealand Nitric Oxide Optics Oryctolagus cuniculus Pacemakers Peptides Performance Permeability Pilot Projects Plasma Proteins Polyethylene Glycols Potential Energy Procedures Process Proteins Protocols documentation Reaction Time Reading Research Research Project Grants Scientific Advances and Accomplishments Signal Transduction Site Students Surface System Techniques Technology Testing Time Tissues Training Troponin T Valine Venous system Viral Tumor Antigens Week Work base biomaterial compatibility concept density design fluorophore implantation implanted sensor improved in vivo knowledge base novel prevent protein structure research study response sensor sialosyl-T antigen

项目摘要

DESCRIPTION (provided by applicant): Troponin-T is an early and specific marker of myocardial infarction. Research has shown that the Troponin-T concentrations significantly increase within the first hour of an impending attack. Since the time to intervene is most critical, an implantable sensor that can detect the onset of infarction by increased Troponin-T level, could result in salvage of the myocardium. Preliminary results in our laboratory have shown the feasibility of a optical resonance energy transfer (RET) sensor to detect Troponin-T, but the biocompatibility and response of the sensor in blood need to be addressed. The goal of this research is to fabricate and characterize a Troponin-T biosensor while engineering surface modifications to enhance biocompatibility. The central hypothesis is that enhanced sensor performance and improved biocompatibility can be achieved by integrating Troponin-T biological sensing elements directly into the surface modification so that coatings can be avoided. The specific aims of this project are to: (1) design and develop a surface modified biosensor that can detect Troponin-T levels in blood, (2) achieve optimal energy transfer for strong signal response by optimizing and characterizing the grafting density and functionality of the immobilized peptides and biological sensing elements, and (3) perform in vivo studies to assess blood-material compatibility and sensor response. The information gained will advance the scientific knowledge of implantable biosensor design and drive the field to further investigate surface modifications for a variety of implantable sensors.

描述（由申请人提供）：Troponin-T是心肌梗塞的早期和特定标记。研究表明，在即将发生攻击的第一小时内，肌钙蛋白-T浓度显着增加。由于干预的时间最关键，因此可以通过增加肌钙蛋白-T水平检测梗塞发作的可植入传感器可能会导致心肌的挽救。我们实验室的初步结果表明，光学共振能量转移（RET）传感器可检测肌钙蛋白T，但需要解决血液中传感器的生物相容性和反应。这项研究的目的是在工程表面修饰时制造和表征肌钙蛋白-T生物传感器，以增强生物相容性。中心假设是，可以通过将肌钙蛋白-T生物传感元件直接整合到表面修饰中来实现增强的传感器性能和提高的生物相容性，从而可以避免涂层。该项目的具体目的是：（1）设计和开发一个可以检测血液中曲霉蛋白T水平的表面修饰的生物传感器，（2）通过优化和表征固定化肽和生物学感应元素的嫁接密度和功能性来实现最佳的能量转移，以实现强信号响应，并评估血液材料的响应，并评估血液材料的响应。获得的信息将提高可植入生物传感器设计的科学知识，并推动该领域，以进一步研究各种可植入传感器的表面修饰。