Computational Foundations for Comparative RNA Sequence and Structure

比较 RNA 序列和结构的计算基础

• 批准号: 7508627
• 负责人: Ron Elber
• 金额: $ 27.2万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-11 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7508627
• 关键词: Algorithms; Archaea; Area; Back; Bacteria; Biological; Cell physiology; Cells; Code; Collection; Communities; DNA; Data; Database Management Systems; Databases; Development; Disease; Elements; Eukaryota; Eukaryotic Cell; Family; Foundations; General Population; Generations; Generic Drugs; Genes; Genome; Goals; Helix (Snails); High Performance Computing; Higher Order Chromatin Structure; Internet; Introns; Knowledge; Laboratory Study; Methods; MicroRNAs; Modeling; Molecular Biology; Numbers; Organism; Pharmacotherapy; Phylogenetic Analysis; Probability; Process; Property; Proteins; Protocols documentation; Public Health; RNA; RNA Sequence Analysis; RNA Sequences; RNA analysis; Regulation; Research; Research Infrastructure; Resources; Restart; Retrieval; Ribosomes; Sequence Alignment; Sequence Analysis; Sequence Homologs; Services; Site; Solvents; Structure; Subgroup; Surface; Taxonomy; Transfer RNA; Variant; Work; base; comparative; concept; data management; data modeling; ear helix; fitness; improved; novel; statistics

DESCRIPTION (provided by applicant): RNA is in the center of a major paradigm shift in Molecular Biology. Our previous perspective of a gene and the general concept for how a cell codes for proteins from DNA is still generally true for Bacteria and Archaea. However, in Eukaryotes, this perspective is being replaced with the notion that the complexity of an organism scales with the amount of RNA, not Protein, that is coded for in its genome. The functional properties of these RNA molecules are directly associated with their higher-order structure. Thus the prediction and identification of this structure from sequence will provide the foundation to understand the details of their function. Since the structural and functional properties of some recently discovered RNAs, such as different micro RNAs, are involved in the regulation of normal and aberrant cellular processes, studies that focus on RNA are expected to help determine the molecular biology for different diseases, and provide the basis for new drug therapies. Comparative analysis of RNA sequences and structures have been very successful in the elucidation of the entire structure of an RNA molecule and in the identification and characterization of the basic building blocks of RNA - structural motifs and structural elements. One of the PIs of this proposal, has established the "Comparative RNA Web (CRW)" site that disseminates collections and analysis of different types of comparative information for a few select RNA molecules. However, based on the significant increase in the number of sequences for the initial set of RNAs studied in this project, and now the significant increase in the number of RNAs that are being identified, we are developing a novel computational foundation intended to establish repeatable protocols for the comparative analysis of RNA sequences and structures. The current CRW Site will become a fully dynamic web service for the general scientific community to perform comparative analysis on their own RNAs, benefiting from the collection of RNA sequence and structure information and the computational infrastructure at the CRW Site. PUBLIC HEALTH RELEVANDE: Based on the functional importance of RNA and its higher-order structure, we are developing a novel computational foundation for the comparative analysis of RNA. This resource will benefit experimental laboratories that are studying RNAs that are associated with many different normal and aberrant processes in the cell, and could directly influence the development of new drug therapies.

描述（由申请人提供）：RNA是分子生物学主要范式转变的中心。我们以前对基因的看法和细胞如何从DNA编码蛋白质的一般概念对于细菌和细菌仍然普遍适用。然而，在真核生物中，这种观点正在被这样的概念所取代：生物体的复杂性与其基因组中编码的RNA（而不是蛋白质）的数量成正比。这些RNA分子的功能特性与它们的高级结构直接相关。因此，从序列中预测和鉴定这种结构将为了解其功能的细节提供基础。由于最近发现的一些RNA的结构和功能特性，例如不同的microRNA，参与正常和异常细胞过程的调节，因此，专注于RNA的研究有望帮助确定不同疾病的分子生物学，并为新的药物治疗提供基础。RNA序列和结构的比较分析在阐明RNA分子的整个结构以及鉴定和表征RNA的基本构建块-结构基序和结构元件方面非常成功。该提案的一个PI建立了“比较RNA网（CRW）”网站，该网站传播一些选定RNA分子的不同类型比较信息的收集和分析。然而，基于本项目中研究的初始RNA组序列数量的显著增加，以及现在正在鉴定的RNA数量的显著增加，我们正在开发一种新的计算基础，旨在建立可重复的RNA序列和结构比较分析方案。目前的CRW网站将成为一个完全动态的网络服务，供一般科学界对自己的RNA进行比较分析，受益于CRW网站收集的RNA序列和结构信息以及计算基础设施。与公共卫生有关的问题：基于RNA及其高阶结构的功能重要性，我们正在开发一种新的计算基础，用于RNA的比较分析。这一资源将使正在研究与细胞中许多不同的正常和异常过程相关的RNA的实验室受益，并可能直接影响新药物疗法的开发。