Bayesian Statistics and Algorithms for Homology Modeling

描述(由申请人提供)：我们的主要目标是改进蛋白质结构预测方法，以开发生物相关状态的蛋白质模型。这些状态可以包括作为同源低聚物的目标蛋白；与其他蛋白质、核酸和配体络合；通过磷酸化和糖基化共价修饰；以及以交替的生理相关构象。关于任何一个靶的这些状态的结构的信息可以来自许多不同的模板；这些信息可以组装成一个复合模型，从该复合模型可以做出生物推断。下一代依赖骨架的旋转异构体文库将使用经典和贝叶斯非参数统计方法开发，并将扩展到包括蛋白质修饰，如磷酸化和糖基化氨基酸。电子密度分析将用于排除具有不确定或动态构象的残基。所得文库将被纳入我们广泛使用的下一代侧链预测程序SCWRL中。将建立一个非常通用的结构生物信息学平台，以便能够在常规基础上对蛋白质结构进行统计和构象分析。我们建议开发交互方法和软件来制作具有生物意义的蛋白质和蛋白质复合体模型，基于多重结构比对、隐马尔可夫模型和来自不同结构的组合信息-配体结合和非结合结构、单体和同源低聚物以及蛋白质复合体。蛋白质结构及其复合体的项目叙事知识对于理解功能和机制是至关重要的。我们将开发用于预测生物相关状态的结构的算法、数据库和软件，包括同源低聚物、翻译后修饰和蛋白质复合体。这些方法将用于通过预测与疾病有关的蛋白质来改善人类健康。

DESCRIPTION (provided by applicant): Our main goal is to improve protein structure prediction methods in order to develop models of proteins in biologically relevant states. Such states may include the target protein as a homo-oligomer; complexed with other proteins, nucleic acids, and ligands; covalently modified through phosphorylation and glycosylation; and in alternate physiologically relevant conformations. Information on the structure of these states for any one target may come from a number of different templates; this information can be assembled into a composite model from which biological inferences can be made. The next generation of the backbone-dependent rotamer library will be developed using classical and Bayesian non-parametric statistics, and it will be extended to include protein modifications, such as phosphorylated and glycosylated amino acids. Electron density analysis will be used to exclude residues with uncertain or dynamic conformations. The resulting libraries will be incorporated into the next generation of our widely used side-chain prediction program SCWRL. A very general structural bioinformatics platform will be constructed to enable statistical and conformational analysis of protein structures on a routine basis. We propose to develop interactive methods and software for producing biologically meaningful models of proteins and protein complexes, based on multiple structure alignments, hidden Markov models, and combined information from diverse structures - ligand-bound and unbound structures, monomers and homo-oligomers, and protein complexes. Project narrative Knowledge of protein structures and their complexes is vital to understanding function and mechanism. We will develop algorithms, databases, and software for predicting structure in biologically relevant states, including homo-oligomers, post-translational modifications, and protein complexes. These methods will be used to improve human health through the prediction of proteins involved in disease.