Global Analysis of Treponema pallidum Antigens

梅毒螺旋体抗原的整体分析

基本信息

批准号：
7201562
负责人：
STEVEN J. NORRIS
金额：
$ 7.06万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-03-15 至 2008-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7201562
关键词：
Africa African American Antigenic Variation Antigens Area Asia Azithromycin Binding Blood Vessels Cardiovascular system Cell model Characteristics Chronic Country Development Diagnosis Diagnostic Disease Epidemic Female Figs - dietary Focal Infection Globus Pallidus Granuloma Health Health Benefit Heterosexuals Human Immune response Immunity Immunological Diagnosis In Vitro Incidence Individual Infection Infection prevention Invasive Lead Lesion Location Macrolide-resistance Malignant Neoplasms Medical Economics Neurosyphilis Not Hispanic or Latino Numbers Order Spirochaetales Organism Oryctolagus cuniculus Paresis Patients Penicillins Pharmaceutical Preparations Pinta Population Prisons Property Protein Array Proteins Public Health Purpose Rate Reporting Research Scourge Screening procedure Secondary Lesion Serological Serum Sexually Transmitted Diseases Skin Social Impacts Specialist Staging Surface Syphilis Tabes Dorsalis Testing Therapeutic Time Treatment Failure Treponema Treponema pallidum Tuberculosis USSR United States Vaccination Vaccines Woman World Health Organization World War II Yaws improved latent infection male men novel pathogen pathogenic bacteria response sex

项目摘要

DESCRIPTION (provided by applicant): Syphilis is a chronic, multistage infection caused by the spirochete Treponema pallidum subsp. pallidum and still represents a major health problem in the United States and worldwide. Inability to culture T. pallidum in vitro has hampered the study of factors involved in host-pathogen interactions. A global analysis of important T. pallidum antigens would be an important step forward in understanding the immune response to infection, and may lead to the identification of proteins useful in immunodiagnosis of syphilis or, potentially, prevention of infection through vaccination. In previous studies, a global screening of 908 T. pallidum gene products in a protein array identified a subset of proteins that are highly reactive with serum from syphilis patients and T. pallidum-infected rabbits. The purpose of the proposed studies is to determine the importance of these antigens in the immune response against T. pallidum infection and in host-pathogen interactions. In Specific Aim 1, the reactivity of newly identified, highly reactive T. pallidum antigens with a panel of sera from human syphilis patients, and hence their importance in the immune response and potential use in immunodiagnosis, will be determined. In Specific Aim 2, we will assess the importance of these novel T. pallidum antigens in host-pathogen interactions by determining immunoprotection in the rabbit model and cellular location and binding properties of the antigens. In summary, syphilis is a sexually transmitted disease that, despite the availability of effective therapy, has a major medical, economic, and social impact both in the United States and other countries. The public health benefit of this project will be the identification of antigens that may improve the diagnosis of syphilis and may lead to the development of an effective vaccine against the disease.

描述（由申请人提供）：梅毒是由螺旋体treponema Pallidum subsp引起的慢性多稳定感染。 Pallidum，仍然代表着美国和全球的主要健康问题。体外无法培养粒子的palliDum t. pallidum to妨碍了对宿主病原体相互作用涉及的因素的研究。对重要的T. pallidum抗原的全球分析将是理解感染免疫反应的重要一步，并可能导致鉴定有助于梅毒免疫诊断的蛋白质，或者，有可能通过疫苗预防感染。在先前的研究中，在蛋白质阵列中对908 T. pallidum基因产物的全球筛查确定了一部分蛋白质，这些蛋白质与来自梅毒患者的血清和T. pallidum感染的兔子具有高度反应性。拟议的研究的目的是确定这些抗原在针对T. pallidum感染和宿主病原体相互作用中的免疫反应中的重要性。在特定的目标1中，将确定新鉴定出的高度反应性T. pallidum抗原与人类梅毒患者的血清面板的反应性，因此将确定它们在免疫反应和免疫诊断中潜在使用中的重要性。在特定的目标2中，我们将通过确定兔模型和细胞位置的免疫保护以及抗原的结合特性来评估这些新型T. pallidum抗原在宿主 - 病原体相互作用中的重要性。总而言之，梅毒是一种性传播疾病，尽管有效治疗，但在美国和其他国家都有重大的医疗，经济和社会影响。该项目的公共卫生益处将是鉴定可以改善梅毒诊断的抗原，并可能导致开发针对该疾病的有效疫苗。