FACS Core

流式细胞仪核心

• 批准号: 7336597
• 负责人: John David FARRAR
• 金额: $ 12.89万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336597
• 关键词: 5-(6)-carboxyfluorescein diacetate succinimidyl ester; Antibodies; Arthritis; Arts; Autoimmune Process; Biological Assay; Cell Lineage; Cell Separation; Cell surface; Cells; Collaborations; Compatible; Cost Savings; Custom; Data; Data Analyses; Data Files; Equipment; Equipment and supply inventories; Experimental Designs; Financial compensation; Flow Cytometry; Fluorochrome; Goals; Housing; Immunology; Individual; Label; Laboratories; Link; Modeling; Molecular Genetics; Mus; Myeloid Cells; Numbers; Online Systems; Pathogenesis; Phosphotransferases; Pliability; Population; Preparation; Principal Investigator; Process; Productivity; Quality Control; Rate; Reagent; Research; Research Activity; Research Infrastructure; Research Personnel; Resources; Sampling; Scientist; Services; Signal Transduction; Signaling Molecule; Sorting - Cell Movement; Speed; Staining method; Stains; Support of Research; Surface; System; Systemic Lupus Erythematosus; Techniques; Testing; Time; Titrations; Work; base; comparative; cost; cytokine; data management; density; discount; experience; member; novel; programs

The Flow Cytometry Core will operate within the existing infrastructures of the Center for Immunology and the Harold C. Simmons Arthritis Research Center to support the research activities of Program investigators. The individual research programs proposed by these investigators have common requirements for reagents and services . The Core will consist of a Director, a Postdoctoral Research Scientist and a Research Technician. The Core will include a laboratory for preparation of samples and equipment in the Flow Cytometry Laboratory of the Immunology Center. A goal of this Core is to expand the polychromatic flow cytometry from cell surface lineage molecules to markers that include intracellular cytokines and kinases. The proposed activities of the Core will provide common and custom reagents and services that would not otherwise be available or economically feasible for the individual laboratories. The specific programs of the Core are: 1. Antibody Labeling and Resource Center 2. Quality Control Laboratory 3. Multiparameter Flow Cytometry Service Close collaboration between the Core and the principal investigators for the individual projects will be maintained in order to optimize their research productivity through utilization of Core reagents and services. In this way, redundancy will be avoided and research reagents will be provided at a reduced cost. This will allow for the centralization of certain techniques, such as high speed sorting, polychromatic flow cytometry and cytometric bead arrays, allowing for quality control and more rapid throughput.

流式细胞术核心将在中心现有基础设施内运行， 免疫学和Harold C.西蒙斯关节炎研究中心支持这项研究 项目调查员的活动。这些研究人员提出的个人研究计划 调查人员对试剂和服务有共同的要求。核心将由一个 主任、博士后研究科学家和研究技术员。核心将包括一个 流式细胞术实验室的样品制备实验室和设备 免疫学中心。该核心的一个目标是将多色流式细胞术从细胞 表面谱系分子与包括细胞内细胞因子和激酶在内的标记物。拟议 核心活动将提供通用和定制试剂和服务， 否则，对于各个实验室来说，这些数据是可用的或经济上可行的。具体 核心方案包括： 1.抗体标记和资源中心 2.质量控制实验室 3.多参数流式细胞术服务 核心小组与各项目的主要调查员之间的密切合作将 通过使用核心试剂来优化其研究生产力 和服务通过这种方式，将避免冗余，并以最低成本提供研究试剂。 降低成本。这将允许集中某些技术，如高速分拣， 多色流式细胞仪和流式细胞仪微珠阵列，允许质量控制等 快速吞吐量。