Identifying antigens against antibodies produced in lesional synovium of rheumatoid arthritis and application of the enzyme-labeled antigen method

为了鉴定抗类风湿性关节炎（RA）滑膜炎产生的抗体的抗原，用alphasgreen方法筛选血清和滑膜组织提取物中抗体识别的抗原。为了进行筛选，利用小麦无生殖细胞蛋白合成系统构建了人蛋白文库。结果，检测到许多蛋白质。将滑膜炎组织中部分高信号抗原应用于酶标记抗原法。类风湿性关节炎滑膜炎患者浆细胞中可见两种抗原的特异性抗体。本研究表明，用AlphaScreen方法筛选蛋白库对检测病变组织中的抗体是有用的。

To identify antigens against antibodies produced within rheumatoid arthritis (RA) synovitis, antigens recognized by antibodies in sera and synovial tissue extracts were screened with the AlphaScreen method. For screening, human protein library was constructed by the wheat germ cell-free protein synthesis system. As a result, a number of proteins were detected. A part of antigens showing high signals in synovitis tissue were applied to the enzyme-labeled antigen method. Specific antibodies against two antigens were visualized in plasma cells in RA synovitis. The present study indicated the usefulness of screening of the protein library with the AlphaScreen method for detecting antibodies in lesional tissue.