Rapid DNA Assay System for Characterization of Infectious Disease Pathogens
用于鉴定传染病病原体的快速 DNA 检测系统
基本信息
- 批准号:7354069
- 负责人:
- 金额:$ 29.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-02-15 至 2009-01-31
- 项目状态:已结题
- 来源:
- 关键词:Anthrax diseaseAreaAutomationBindingBiochemistryBiocompatible Coated MaterialsBiological AssayBiological WarfareCaliberCaringCervix carcinomaChemistryClinicalClinical ManagementCommunicable DiseasesContractsDNADNA SequenceDataDetectionDevelopmentDevicesDiagnosticElementsEpidemiologyFeasibility StudiesGene ChipsGenesGenitourinary systemGenomicsGenotypeGoalsGoldHPV-High RiskHeadHumanHuman PapillomavirusHuman papilloma virus infectionHuman papillomavirus 16Human papillomavirus 18HybridsIndividualKnowledgeLabelLeadLesionLicensingLow risk HPVMagnetismMalignant NeoplasmsMalignant neoplasm of cervix uteriMedical SurveillanceMemoryMethodsMicrobeMinorModificationMolecularNoiseNumbersObject AttachmentOligonucleotidesOutcomePapillomavirusPathogen detectionPatientsPhasePoint-of-Care SystemsPolymerase Chain ReactionPredispositionPreparationPrincipal InvestigatorProtocols documentationRNA ProbesReactionReadingResourcesRiskSamplingSatellite VirusesScreening procedureSensitivity and SpecificitySignal TransductionSilicon DioxideSolutionsSpecificitySurfaceSystemTechnologyTestingTimeTubeUniversitiesVariantViralanticancer researchassay developmentbasecostdensitydesigndesiredetectorgenome sequencinghuman femaleinsightmalignant mouth neoplasmmicrochipnanoparticlenew technologynovelnucleic acid detectionpathogenpreventprogramsreconstructionresearch and developmentresearch studysensorsuccesstooltumorigenesisviral DNAvirus related cancer
项目摘要
DESCRIPTION (provided by applicant): We propose, using a high-sensitivity MagArray DNA detection system, to develop a rapid assay for infectious disease pathogens. In particular we will develop an assay for a large number of HPV genotypes using the MagArray platform. The viral origin of cervical cancer is now well established. Recent studies have shown that Human Papilloma Virus (HPV) DNA can be found in 99.7% of all cervical carcinomas. Because different HPV subtypes carry different risks for the development of cancer, a detection method that can distinguish subtypes could guide clinical care and offer new epidemiological insights. Current detection methods are limited to genotyping 30-35 of the more than 100 HPV types, and this is most often done by PCR. Detection, genotyping and analysis of broad spectrum of HPVs using a small amount of DNA that can be extracted from clinical samples would represent significant progress. Here we propose to adapt the MagArray platform to detect a broad spectrum (all types) of HPVs. The MagArray platform is based on using magnetic nanoparticles to tag the target fragments, rather than fluorescent molecules, as is the present practice. The advantage of using magnetic nanoparticles (typically 15 nm in diameter) as tags rather than fluorescent molecules is an enormous increase in sensitivity. With fluorescent labeling, one typically needs 100,000 fluorescently labeled molecules to obtain a signal/noise ratio adequate for reliable identification. MagArray technology will serve as a sensitive, specific diagnostic tool to detect multiple HPV types. In the first year feasibility studies will be performed for the high-risk genotypes HPV-16 and HPV-18 associated with cervical cancer. In the second year, we will determine the sensitivity and selectivity of at least 20 HPV types with a single sample, representing a large portion of the known types, most of which are strongly associated with anogenital and oral cancers. All these HPV types will be detected in a single experiment. This will reduce the cost, the time required for typing, and the amount of DNA sample required on a per-type basis. The application and development of MagArray technology to HPV-related cancer will serve as a basis for many applications in other infectious disease and cancer research and related clinical management. This technology has the potential to be integrated in a compact device that is simple and easy to use as a Point-of-Care system for molecular diagnostic applications.
描述(由申请人提供):我们建议使用高灵敏度MagArray DNA检测系统开发一种快速检测传染病病原体的方法。特别是,我们将使用MagArray平台开发用于大量HPV基因型的检测。宫颈癌的病因是什么?最近的研究表明,人乳头瘤病毒(HPV)DNA可以在99.7%的宫颈癌中找到。由于不同的HPV亚型对癌症的发展具有不同的风险,因此可以区分亚型的检测方法可以指导临床护理并提供新的流行病学见解。目前的检测方法仅限于对100多种HPV类型中的30-35种进行基因分型,这通常通过PCR进行。利用从临床样本中提取的少量DNA进行广谱HPV的检测、基因分型和分析将代表重大进展。在这里,我们建议调整MagArray平台,以检测广谱(所有类型)的HPV。MagArray平台基于使用磁性纳米颗粒来标记靶片段,而不是像目前的实践那样使用荧光分子。使用磁性纳米颗粒(通常直径为15 nm)作为标记而不是荧光分子的优点是灵敏度的巨大增加。使用荧光标记,通常需要100,000个荧光标记的分子以获得足以进行可靠鉴定的信噪比。MagArray技术将作为一种灵敏、特异的诊断工具,用于检测多种HPV类型。在第一年,将对与宫颈癌相关的高危基因型HPV-16和HPV-18进行可行性研究。在第二年,我们将用一个样本确定至少20种HPV类型的敏感性和选择性,代表了已知类型的很大一部分,其中大多数与肛门生殖器癌和口腔癌密切相关。所有这些HPV类型都将在一次实验中检测到。这将减少分型所需的成本和时间,以及每个类型所需的DNA样本数量。MagArray技术在HPV相关癌症中的应用和发展将为其他传染病和癌症研究以及相关临床管理的许多应用奠定基础。这项技术有可能被集成在一个紧凑的设备,是简单和易于使用的点的护理系统的分子诊断应用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Robert Lee White其他文献
Robert Lee White的其他文献
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{{ truncateString('Robert Lee White', 18)}}的其他基金
Rapid DNA Assay System for Characterization of Infectious Disease Pathogens
用于鉴定传染病病原体的快速 DNA 检测系统
- 批准号:
7216434 - 财政年份:2007
- 资助金额:
$ 29.67万 - 项目类别:
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