Chemical profiling of biological cell / engineering alloy interactions

生物细胞/工程合金相互作用的化学分析

• 批准号: EP/E015379/1
• 负责人: Paul Brown
• 金额: $ 20.39万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE015379%2F1
• 关键词: Chemical; profiling; biological; cell; engineering

There have been many exciting advances in ceramic, polymeric or composite based biomaterials in recent years. However, the vast majority of biomedical implants still remain metallic-based, covering a wide range of sites and time dependencies in the body. Titanium-based alloys, for example, may be used within orthopaedic and osteosynthesis devices such as hip and knee joints, bone fixation plates and screws, along with heart valves, maxillofacial implants, dental implants and potentially vascular stents. Even though there is a huge industry dedicated to the development and application of implant materials, there is still a clear gap in our knowledge as to the way a biological environment responds to a biomaterial surface. Thus, continued development and improved understanding of candidate implant materials on the molecular-scale is required.The project aim is the development of robust methodologies to enable the initial response of biomedical implants to soft and hard tissue environments to be chemically profiled on the molecular scale. To achieve this, we propose to conduct a feasibility study to characterise the very first stages of biological cell / engineering alloy interaction using cross-sectional transmission electron microscopy (TEM), through the subtle development of established sample preparation procedures. In this context, a variety of microanalytical assessment techniques, including electron spectroscopic imaging (ESI) and energy dispersive X-ray spectroscopy (EDS), will be used to chemically map the corrosion processes of a range of model implant templates, including Ti, Ti-based alloys and NiTi, and stainless steel, CoCr and hydroxyapatite, exposed in-vitro to human osteoblast and endothelial cell cultures; intermediate small cell derived platelets; and serum protein and simulated body-fluid. Emphasis will also be given to the assessment by the complementary techniques of environmental scanning electron microscopy (ESEM) and optical fluorescence microscopy. The approach is to combine our in-house expertise in the areas of biomaterials deposition, surface modification, cell culturing and materials characterisation.

近年来，基于陶瓷、聚合物或复合材料的生物材料取得了许多令人振奋的进展。然而，绝大多数生物医学植入物仍然是以金属为基础的，覆盖了人体内广泛的部位和时间依赖性。例如，钛基合金可用于矫形和骨接合设备，如髋关节和膝关节、骨固定板和螺丝，以及心脏瓣膜、颌面部植入物、牙科植入物和潜在的血管支架。尽管有一个巨大的行业致力于植入材料的开发和应用，但在生物环境对生物材料表面的响应方式方面，我们的知识仍然存在明显的差距。因此，需要在分子尺度上继续开发和提高对候选植入材料的理解。该项目的目标是开发稳健的方法学，使生物医学植入物对软组织和硬组织环境的初始响应能够在分子尺度上进行化学描述。为了实现这一目标，我们建议进行一项可行性研究，通过微妙地开发已建立的样品制备程序，使用横截面透射电子显微镜(TEM)来表征生物细胞/工程合金相互作用的第一阶段。在此背景下，包括电子光谱成像(ESI)和能量色散X射线光谱(EDS)在内的各种微观分析评估技术将被用于绘制一系列模型植入模板的腐蚀过程的化学图谱，这些模板包括钛、钛基合金和NiTi，以及不锈钢、CoCr和羟基磷灰石，它们在体外暴露于人成骨细胞和内皮细胞培养；中等小细胞来源的血小板；以及血清蛋白和模拟体液。重点还将通过环境扫描电子显微镜(ESEM)和光学荧光显微镜的互补技术进行评估。我们的方法是结合我们在生物材料沉积、表面修饰、细胞培养和材料表征领域的内部专业知识。

项目成果

EMC 2008 14th European Microscopy Congress 1-5 September 2008, Aachen, Germany

EMC 2008 第 14 届欧洲显微镜大会 2008 年 9 月 1-5 日，德国亚琛

• DOI: 10.1007/978-3-540-85156-1_238
• 发表时间: 2008
• 作者: Fay M

Correction to: Increasing of activity and thermostability of cold active butanol-tolerant endoglucanase from a marine Rhodococcus sp. under high concentrations of butanol condition.

修正：海洋红球菌属耐冷丁醇内切葡聚糖酶的活性和热稳定性增加。

• DOI: 10.1007/978-3-540-85156-1_331
• 发表时间: 2018
• 期刊: 3 Biotech
• 影响因子: 2.8
• 作者: Zeng X