Assembly and Structure of Bacteriophage Lambda

噬菌体 Lambda 的组装和结构

• 批准号: 7477845
• 负责人: ROGER W HENDRIX
• 金额: $ 50万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 2009-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7477845
• 关键词: Address; Affect; Animals; Bacteriophage lambda; Bacteriophages; Biochemical; Biological; Capsid Proteins; Collaborations; Complex; DNA Packaging; DNA Sequence Rearrangement; Endopeptidases; Escherichia coli; Fiber; Head; Intervention; Length; Measures; Mediating; Molecular; Molecular Chaperones; Pathway interactions; Peptide Hydrolases; Plant Viruses; Process; Proteins; Resolution; Resources; Site; Site-Directed Mutagenesis; Structure; Tail; Viral; interest; movie; receptor binding

DESCRIPTION (provided by applicant): The dsDNA bacteriophages provide an unparalleled resource for investigating the principles of biological assembly at the molecular level. This is a proposal to continue the study of these problems using the E. coli bacteriophages lambda and HK97. Specific interests include assembly of the heads of HK97, with an emphasis on the extensive conformational rearrangement in the capsid protein that happens during DNA packaging, assembly of the phage lambda tail, with an emphasis on the newly discovered chaperone-mediated step in the assembly of the tail length template, and assembly and structure of the lambda long tail fibers. Information gained in this study will be generalizable beyond bacteriophages and will therefore affect our understanding of, among others, assembly processes in animal and plant viruses, which are possible points for anti-viral intervention.

描述（由申请方提供）：dsDNA噬菌体为研究分子水平上的生物组装原理提供了无与伦比的资源。这是一个建议，继续研究这些问题，使用E。大肠杆菌噬菌体λ和HK97。具体的利益包括装配的头部HK97，强调广泛的构象重排的衣壳蛋白，发生在DNA包装，装配的噬菌体λ尾，强调新发现的伴侣介导的步骤中的装配的尾长模板，和装配和结构的λ长尾纤维。 在这项研究中获得的信息将是一般化的超越噬菌体，因此将影响我们的理解，除其他外，在动物和植物病毒，这是抗病毒干预的可能点组装过程。