Host Immune Responses to Antigens of Malaria Parasites

宿主对疟疾寄生虫抗原的免疫反应

• 批准号: 7592371
• 负责人: Carole Long
• 金额: $ 57.05万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592371
• 关键词: Adult; Africa; Animals; Antibody Formation; Antigens; Area; Biological Assay; Biological Models; Blood; CD4 Positive T Lymphocytes; Cells; Child; Clinical; Development; Disease; Erythrocytes; Goals; Growth; Head; Human; Immune response; Immune system; Infection; Inflammation; Intervention; Investigation; Laboratories; Life; Malaria; Malaria Vaccines; Mali; Parasites; Pathology; Phenotype; Research; Rodent Model; Serum; Specificity; Staging; T memory cell; T-Lymphocyte; Techniques; Vaccines; novel; response; tool; vaccine development; vector

In the spring of 2007, the Long lab moved from the Malaria Vaccine Development Branch (MVDB) to the Laboratory of Malaria and Vector Research (LMVR). Since then we have set up and equipped the laboratory, established rodent model systems of malaria infection, and with the support of LMVR and DIR, have established a laboratory in Mali headed by Dr. Mahamadou Diakite. This now allows us to expand our investigations into the interface between the malaria parasite and the host innate and adaptive immune systems. In this context we have continued our ongoing analyses of animal and human sera directed to malaria parasite antigens using a standardized parasite growth inhibition assay that we have developed; this has contributed significantly to decisions about clinical development of various blood-stage antigens. Also, we have applied the assay as a research tool to identify novel erythrocytic-stage vaccine candidates. On the cellular level we have established techniques for analysis of human CD4+ T cell responses to malaria antigens including identification of T memory cells and T cells with a regulatory phenotype. These approaches can now be transitioned to analysis of cells from children and adults in malaria-endemic areas of Mali. Finally, we have initiated studies on innate immune responses to malaria parasites in rodent models and we will be moving these into the field as well.

在2007年春季，Long Lab从疟疾疫苗开发部门（MVDB）转移到了疟疾和载体研究实验室（LMVR）。从那时起，我们已经建立并配备了实验室，已建立的疟疾感染啮齿动物模型系统，并在LMVR和DIR的支持下，在Mahamadou Diakite博士领导的马里建立了一个实验室。 现在，这使我们能够将调查扩展到疟原虫寄生虫与宿主先天和适应性免疫系统之间的界面。在这种情况下，我们继续使用我们已经开发的标准化寄生虫生长抑制测定法进行了对针对疟疾寄生虫抗原的动物和人类血清的持续分析。这对各种血阶段抗原的临床发育的决策做出了重大贡献。此外，我们将该测定方法应用于研究工具，以鉴定新型的红细胞疫苗候选物。在细胞水平上，我们建立了用于分析人类CD4+ T细胞对疟疾抗原的反应的技术，包括用调节表型鉴定T记忆细胞和T细胞。现在，这些方法可以转变为对马里疟疾 - 流行地区儿童和成人的细胞分析。最后，我们开始了关于啮齿动物模型中对疟疾寄生虫的先天免疫反应的研究，我们也将将这些反应移至该领域。