Multianalyte channel biosensor

这个博士后流动性项目的目的是展示微通道流动池在全分析系统（microTAS）中的潜力，即护理点生命科学应用。我们的目标是将一种新型的多重免疫测定集成到微通道流动池中，用于同时测量在疾病状态诊断中起关键作用的多种生物标志物和其他抗原分析物。所提出的在microTAS流动池中进行多重免疫测定的方案包括以夹心或竞争性免疫测定形式对抗体进行金属离子标记，以及电化学捕获和阳极溶出分析。一个关键问题是微通道流动池的设计，其可以被定制为具有不同的长度、宽度、高度和流速，以控制通道中不同点处的反应物浓度。该分析的成功在于根据抗原-抗体反应的结合动力学和标记抗原/生物标志物的捕获效率来定制通道。该项目的主要重点将是量化生物标志物/金属标记生物标志物的浓度与所设计的通道流动池内的微带传感电极上捕获的金属的剥离电荷之间的关系。最初，将用单一生物标志物评估所设计的细胞的性能，并建立单一免疫测定的实验条件。之后，所提出的方法的实验评价将扩展到在所设计的通道流动池中同时测定具有不同金属标记的多个生物标志物。

The aim of this postdoctoral mobility project is to demonstrate the potential of a microchannel flow cell in a total analytical system (microTAS), point of care life sciences application. The goal is to integrate a novel multiplexed-immunoassay into a micro channel flow cell, for the simultaneous measurement of multiple biomarkers and other antigenic analytes that play a key role in the diagnosis of disease state. The proposed scheme for multiplexed immunoassay in a microTAS flow cell includes metal ion labelling of the antibody in a sandwich or competitive immunoassay format, together with electrochemical capture and anodic stripping analysis. A key issue is the design of the microchannel flow cell that can be tailored with different lengths, widths, heights and flow rates to control concentration of reactants at different points in the channel. The success of the assay is the tailoring of the channel to the binding kinetics of the antigen-antibody reaction and capture efficiency of the labelled antigen/biomarker.The main focus of the project will be on quantifying relationships of the concentrations of biomarkers /metal-labelled biomarker and the striping charge of captured metal on the microband sensing electrodes within the designed channel flow cells. Initially the performance of the designed cells will be evaluated with a single biomarker, and the experimental conditions for single immunoassay established. Afterwards, the experimental evaluation of the proposed methodology will be extended for simultaneous determination of multiple biomarkers with different metal labels in the designed channel flow cell.