Thin Polyelectrolyte Films For Controlled Surface-Cell Interactions

用于控制表面细胞相互作用的聚电解质薄膜

• 批准号: 7346919
• 负责人: JOSEPH B. SCHLENOFF
• 金额: $ 25.54万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7346919
• 关键词: Adhesions; Adhesives; Adsorption; Area; Behavior; Biological; Blood Platelets; Bulla; Cell Adhesion; Cell Adhesion Molecules; Cell Communication; Cells; Cellular biology; Chemicals; Chemistry; Cues; Cytoskeletal Proteins; Disulfides; Electrostatics; Evaluation; Extracellular Matrix; Eye; Feedback; Fibroblasts; Fibronectins; Film; Goals; Immunofluorescence Immunologic; Implant; Investigation; Lateral; Localized; Manuscripts; Measurement; Mechanics; Methods; Microscopy; Modality; Muscle; Muscle Rigidity; Nature; Organism; Oxidants; Paper; Phenotype; Photochemistry; Play; Polymers; Preparation; Property; Proteins; Publishing; Purpose; Research Personnel; Scanning Probe Microscopes; Science; Smooth Muscle Myocytes; Stretching; Sulfhydryl Compounds; Surface; Surface Properties; System; Techniques; Technology; Testing; Tissue Engineering; Work; cell behavior; cell motility; cell type; copolymer; crosslink; functional group; hydrophilicity; implant coating; insight; interest; migration; nanomechanical; novel; physical chemical interaction; programs; promoter; response

DESCRIPTION (provided by applicant): When synthetic or natural materials, such as those used in implants and tissue engineering, come in contact with living systems, they induce specific or general responses in cells and the extracellular matrix. Cells are known to respond to both the chemistry and the mechanical properties of the surface to which they adhere. This project will explore new thin films for regulating cell adhesion and controlling cell behavior. In this work, protein and cell adhesion to ultrathin films made by the polyelectrolyte multilayering technique will be investigated. The study involves the synthesis of new polymers, their assembly, and a coordinated evaluation of the response of cells to these synthetic materials. Functional groups will be introduced into the polymers to control hydrophilicity and crosslinking. Distinction will be made between the nonspecific chemistry presented to the extracellular matrix at the surface of the film, and the film interior, which will impact the mechanical properties. The purpose of this distinction is to separate contributions of nonspecific protein adsorption and film modulus-induced cellular responses to cell adhesion, differentiation, and proliferation. The three interleaving groups of Aims present increasing levels of feedback with biological systems. We start with a goal of producing a minimally adhesive background surface, then we "dope" it with precise levels of adhesion-promoting protein. We continue with an eye towards controlled chemical and physical interactions between cells and substrates. Driven partly by hypothesis and partly by a quest for new materials at the biointerface, we are interested in how cellular organization responds to broad chemical and physical cues. Points of novelty include new materials compositions, migration of a promising technology to the biointerface, methods of crosslinking, and forming gradients in mechanical properties in the plane of the film.

描述（由申请人提供）：当合成或天然材料（例如用于植入物和组织工程的材料）与生命系统接触时，它们会在细胞和细胞外基质中诱导特异性或一般反应。众所周知，细胞会对它们粘附的表面的化学和机械特性做出反应。该项目将探索用于调节细胞粘附和控制细胞行为的新型薄膜。在这项工作中，将研究蛋白质和细胞对聚电解质多层技术制成的超薄膜的粘附。该研究涉及新聚合物的合成、组装以及细胞对这些合成材料反应的协调评估。将官能团引入聚合物中以控制亲水性和交联。将区分薄膜表面细胞外基质和薄膜内部的非特异性化学物质，这将影响机械性能。这种区别的目的是区分非特异性蛋白质吸附和膜模量诱导的细胞反应对细胞粘附、分化和增殖的贡献。目标的三个交错组呈现出与生物系统的反馈水平不断提高。我们的目标是生产最低限度粘附的背景表面，然后用精确水平的粘附促进蛋白质“掺杂”它。我们继续着眼于细胞和基质之间受控的化学和物理相互作用。部分由假设驱动，部分由对生物界面新材料的探索驱动，我们对细胞组织如何响应广泛的化学和物理线索感兴趣。新颖之处包括新材料成分、有前途的技术向生物界面的迁移、交联方法以及在薄膜平面上形成机械性能梯度。