权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Green tea derived EGCG opposes AIDS dementia-like neuronal damage

绿茶提取的 EGCG 可以对抗艾滋病痴呆样神经元损伤

基本信息

批准号：
7460708
负责人：
Jun Tan
金额：
$ 18.38万
依托单位：
UNIVERSITY OF SOUTH FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-05 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7460708
关键词：
AIDS Dementia Complex Attenuated Bax protein Biochemical Biological Assay Brain Brain Ischemia C57BL/6 Mouse Cells Cerebrospinal Fluid Cessation of life Data Development Disruption Dose Exhibits Foundations Future Glycoproteins Goals Green tea HIV Envelope Protein gp120 HIV Seropositivity HIV Transactivator Protein HIV-1 Human Image Analysis Immune In Vitro Individual Inflammatory Injection of therapeutic agent Interferon Type II Interferons Intraperitoneal Injections Ischemia Lactate Dehydrogenase Lead Mediating Modeling Mus Neurodegenerative Disorders Neuroglia Neuronal Injury Neurons Pathology Phosphorylation Phosphotransferases Pilot Projects Principal Investigator Proteins Purpose Research Resistance Role STAT1 protein Signal Pathway Signal Transduction Signaling Molecule Staining method Stains Stroke Testing Therapeutic Therapeutic Effect Transcriptional Activation Transducers Viral Work cell injury cytokine day desire dosage gallocatechol in vivo inhibitor/antagonist mouse model neuropathology neurotoxic neurotoxicity prevent programs prophylactic

项目摘要

DESCRIPTION (provided by applicant): Our long term goal is to develop effective agents for AIDS dementia complex. The purpose of this project is to investigate if green tea EGCG, a known inhibitor of signal transducer and activation of transcription 1 (STAT1), could attenuate AIDS-like neuronal damage through disruption STAT1 signaling in mice exposed to IFN-g with HIV-1 proteins gp120 and Tat. A state of immune activation characterized by increased levels of pro-inflammatory cytokines is widely regarded as an important factor leading to AIDS dementia-like neuronal damage and a key regulator for pro-inflammatory signaling in neurons is STAT1. STAT1 activation is involved in neuronal injury/death associated with brain ischemia, as well as AIDS dementia. Moreover its "activator" IFN-g, has been shown to be elevated both in the cerebrospinal fluid and brains of HIV positive individuals. Results from our pilot studies suggest that IFN-g enhances the neurotoxic effects of gp120 and Tat. Further, AIDS dementia-like neuronal damage from this combined insult was largely attenuated in STAT1 deficient neurons. We and others have shown that the treatment of primary neurons with EGCG reduces STAT1 activation and resultant neuronal injury in models of AIDS dementia and stroke, respectively. We hypothesize that EGCG treatment will decrease AIDS dementia-like neuronal damage via STAT1 suppression. Aim 1 is to establish a mouse model representative of the possible neuronal damage exhibited in AIDS dementia. Aim 2 will characterize the role of STAT1 in mediating neuronal injury induced by IFN-g and gp120 and Tat in mice. To this end we will compare the neuronal damage in STAT1 deficient mice to control mice after intracerebroventricular injection of IFN-g with gp120 or Tat. We expect STAT1 deficient mice will show a marked decrease in AIDS dementia-like neuronal damage compared to control. Aim 3 will investigate the effects of EGCG on inhibition of the neuronal damage resulting from IFN-g mediated STAT1 activation in the presence of gp120 or Tat in mice. Here we also plan to delineate the most effective EGCG intraperitoneal injection by comparing AIDS dementia-like neuronal damage in prophylactic, therapeutic, and combined prophylactic/therapeutic paradigms. We hypothesize that EGCG-treated mice will show significant decreases in AIDS dementia-like neuronal damage when compared to vehicle-treated mice. These data should lay the foundation for further research leading to human trials in the near future. Narrative: The purpose of this project is to investigate if green tea derived EGCG could attenuate AIDS-like neuronal damage in the mouse model. We hypothesize that EGCG-treated mice will show significant decreases in AIDS dementia-like neuronal damage when compared to vehicle-treated mice. These data should lay the foundation for further research leading to human trials in the near future.

描述（由申请人提供）：我们的长期目标是开发治疗艾滋病痴呆症的有效药物。该项目的目的是研究绿茶 EGCG（一种已知的信号转导和转录激活 1 (STAT1) 抑制剂）是否可以通过破坏暴露于含有 HIV-1 蛋白 gp120 和 Tat 的 IFN-g 的小鼠中的 STAT1 信号传导来减轻艾滋病样神经元损伤。以促炎细胞因子水平升高为特征的免疫激活状态被广泛认为是导致艾滋病痴呆样神经元损伤的重要因素，而神经元促炎信号传导的关键调节因子是STAT1。 STAT1 激活涉及与脑缺血以及艾滋病痴呆相关的神经元损伤/死亡。此外，其“激活剂”IFN-g 已被证明在 HIV 阳性个体的脑脊液和大脑中均升高。我们的初步研究结果表明，IFN-g 增强了 gp120 和 Tat 的神经毒性作用。此外，这种联合损伤造成的艾滋病痴呆样神经元损伤在 STAT1 缺陷神经元中很大程度上减弱。我们和其他人已经证明，在艾滋病痴呆和中风模型中，用 EGCG 治疗原代神经元可分别减少 STAT1 激活和由此产生的神经元损伤。我们假设 EGCG 治疗将通过抑制 STAT1 来减少艾滋病痴呆样神经元损伤。目标 1 是建立代表艾滋病痴呆中可能表现出的神经元损伤的小鼠模型。目标 2 将描述 STAT1 在介导 IFN-g、gp120 和 Tat 诱导的小鼠神经元损伤中的作用。为此，我们将比较 STAT1 缺陷小鼠与对照小鼠脑室内注射 IFN-g 与 gp120 或 Tat 后的神经元损伤。我们预计 STAT1 缺陷小鼠与对照小鼠相比，艾滋病痴呆样神经元损伤将显着减少。目标 3 将研究在 gp120 或 Tat 存在的情况下，EGCG 对抑制 IFN-g 介导的 STAT1 激活引起的神经元损伤的影响。在这里，我们还计划通过比较预防性、治疗性和联合预防/治疗范例中的艾滋病痴呆样神经元损伤来描述最有效的 EGCG 腹腔注射。我们假设，与媒介物处理的小鼠相比，EGCG 处理的小鼠的艾滋病痴呆样神经元损伤显着减少。这些数据应该为不久的将来进行人体试验的进一步研究奠定基础。叙述：该项目的目的是研究绿茶提取的 EGCG 是否可以减轻小鼠模型中类似艾滋病的神经元损伤。我们假设，与媒介物处理的小鼠相比，EGCG 处理的小鼠的艾滋病痴呆样神经元损伤显着减少。这些数据应该为不久的将来进行人体试验的进一步研究奠定基础。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Psychiatric implications of hepatitis-C infection.

丙型肝炎感染的精神影响。

DOI：
10.1615/critrevneurobiol.v19.i2-3.20
发表时间：
2007
期刊：
Critical reviews in neurobiology
影响因子：
0
作者：
Giunta,Brian;Somboonwit,Charurut;Nikolic,WilliamV;Rrapo,Elona;Tan,Jun;Shapshak,Paul;Fernandez,Francisco
通讯作者：
Fernandez,Francisco

Antiretroviral medications disrupt microglial phagocytosis of β-amyloid and increase its production by neurons: implications for HIV-associated neurocognitive disorders.