Bioassay Core

生物测定核心

• 批准号: 7479058
• 负责人: Yanbin Dong
• 金额: $ 41.72万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7479058
• 关键词: Albumins; Aldosterone; Angiotensin II; Antioxidants; Biochemical; Biochemistry; Biological Assay; Corticosterone; Corticotropin; Creatinine; DNA; Data; Databases; Electrodes; Electrolytes; Ensure; Enzyme-Linked Immunosorbent Assay; Epinephrine; Equipment; Genes; Genetic; Genetic Polymorphism; Genetic Services; Genetic Techniques; Genome; Genotype; Glucose; High Density Lipoprotein Cholesterol; High Pressure Liquid Chromatography; Human; Hydrocortisone; Hydrogen Peroxide; Individual; Information Storage; Insulin; Knockout Mice; Laboratories; Leptin; Mediation; Methods; Molecular Genetics; Nitrates; Nitrites; Norepinephrine; Plasma; Price; Procedures; Quality Control; Radioimmunoassay; Rattus; Receptor Gene; Receptor, Angiotensin, Type 1; Records; Renin; Research Personnel; Resource Development; Role; Sampling; Schedule; Services; Single Nucleotide Polymorphism; Tail; Techniques; Tissue Extracts; Transforming Growth Factor beta; Transgenic Organisms; Urine; Validation; Validity and Reliability; Wild Type Mouse; Work; data management; design; experience; human CYBA protein; isoprostaglandin F2alpha type-III; low density lipoprotein triglyceride; nitrate; post gamma-globulins; programs; receptor; skills; statistics; tetrahydrobiopterin

The Bioassay Core will provide biochemical assay and genotyping services for the individual projects within the Program Project. Biochemical assays that will be performed include analyses in plasma and urine for human and rat samples. The following assays will be performed: Plasma renin activity, aldosterone, angiotensin II, cortisol, corticosterone, ACTH, nitrite, nitrate, 8-isoprostane, TEARS, antioxidant capacity, hydrogen peroxide, norepinephrine, epinephrine, albumin, electrolytes, creatinine, total cholesterol, HDL, LDL, triglycerides, glucose, insulin, cystatin C, tetrahydrobiopterin, leptin, and transforming growth factor beta. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, ELISA, colorimetric, and ionselective electrodes. Genotyping of tagging single nucleotide polymorphisms of the genes of GCCR, CRHR1, p22phox, and EC-SOD will be performed for Project 2. Genotyping of the C-521T polymorphism of the angiotensin II receptor type 1 gene will be performed for Project 1. Genotyping services will be also performed for Project 3 and 4 using the tail snip DMA of the transgenic rats of the ETB receptor deficient rat and endothelial ET knockout mice. The extracted DMA will be genotyped to determine whether the rats are wildtype, heterozygous, or homozygous for the ETB receptor gene and whether the mice are wild-type, heterozygous, or homozygous for the ET gene. The core will also provide resources for development of additional assays as needed by individual investigators. The core will assure assay quality control through validation procedures.

生物检测中心将为内部的各个项目提供生化检测和基因分型服务