Bioassay Core

生物测定核心

• 批准号: 7479058
• 负责人: Yanbin Dong
• 金额: $ 41.72万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7479058
• 关键词: Albumins; Aldosterone; Angiotensin II; Antioxidants; Biochemical; Biochemistry; Biological Assay; Corticosterone; Corticotropin; Creatinine; DNA; Data; Databases; Electrodes; Electrolytes; Ensure; Enzyme-Linked Immunosorbent Assay; Epinephrine; Equipment; Genes; Genetic; Genetic Polymorphism; Genetic Services; Genetic Techniques; Genome; Genotype; Glucose; High Density Lipoprotein Cholesterol; High Pressure Liquid Chromatography; Human; Hydrocortisone; Hydrogen Peroxide; Individual; Information Storage; Insulin; Knockout Mice; Laboratories; Leptin; Mediation; Methods; Molecular Genetics; Nitrates; Nitrites; Norepinephrine; Plasma; Price; Procedures; Quality Control; Radioimmunoassay; Rattus; Receptor Gene; Receptor, Angiotensin, Type 1; Records; Renin; Research Personnel; Resource Development; Role; Sampling; Schedule; Services; Single Nucleotide Polymorphism; Tail; Techniques; Tissue Extracts; Transforming Growth Factor beta; Transgenic Organisms; Urine; Validation; Validity and Reliability; Wild Type Mouse; Work; data management; design; experience; human CYBA protein; isoprostaglandin F2alpha type-III; low density lipoprotein triglyceride; nitrate; post gamma-globulins; programs; receptor; skills; statistics; tetrahydrobiopterin

The Bioassay Core will provide biochemical assay and genotyping services for the individual projects within the Program Project. Biochemical assays that will be performed include analyses in plasma and urine for human and rat samples. The following assays will be performed: Plasma renin activity, aldosterone, angiotensin II, cortisol, corticosterone, ACTH, nitrite, nitrate, 8-isoprostane, TEARS, antioxidant capacity, hydrogen peroxide, norepinephrine, epinephrine, albumin, electrolytes, creatinine, total cholesterol, HDL, LDL, triglycerides, glucose, insulin, cystatin C, tetrahydrobiopterin, leptin, and transforming growth factor beta. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, ELISA, colorimetric, and ionselective electrodes. Genotyping of tagging single nucleotide polymorphisms of the genes of GCCR, CRHR1, p22phox, and EC-SOD will be performed for Project 2. Genotyping of the C-521T polymorphism of the angiotensin II receptor type 1 gene will be performed for Project 1. Genotyping services will be also performed for Project 3 and 4 using the tail snip DMA of the transgenic rats of the ETB receptor deficient rat and endothelial ET knockout mice. The extracted DMA will be genotyped to determine whether the rats are wildtype, heterozygous, or homozygous for the ETB receptor gene and whether the mice are wild-type, heterozygous, or homozygous for the ET gene. The core will also provide resources for development of additional assays as needed by individual investigators. The core will assure assay quality control through validation procedures.

生物化验中心将为以下各个项目提供生化化验和基因分型服务 计划项目。将进行的生化分析包括血浆和尿液中的 人和老鼠的样本。将进行以下检测：血浆肾素活性、醛固酮、 血管紧张素II，皮质醇，皮质酮，促肾上腺皮质激素，亚硝酸盐，硝酸盐，8-异前列腺素，泪水，抗氧化能力， 过氧化氢，去甲肾上腺素，肾上腺素，白蛋白，电解质，肌酐，总胆固醇，高密度脂蛋白，低密度脂蛋白， 甘油三酯、葡萄糖、胰岛素、胱抑素C、四氢生物蝶呤、瘦素和转化生长因子β。 技术将包括放射免疫分析、放射酶分析、高效液相、酶联免疫吸附试验、比色法和离子选择。 电极。GCCR、CRHR1、GCCR基因标记单核苷酸多态的基因分型 项目2将进行p22Phox和EC-SOD的检测。 血管紧张素II受体1型基因将在项目1中进行。还将进行基因分型服务 对于项目3和项目4，使用ETB受体缺陷大鼠的转基因大鼠的尾部片段DMA和 血管内皮细胞ET基因敲除小鼠。提取的DNA将进行基因分型以确定老鼠是否为野生型， ETB受体基因是杂合还是纯合子，无论小鼠是野生型， ET基因为杂合子或纯合子。核心还将提供资源，以发展 根据个别调查人员的需要进行额外的分析。核心将通过以下方式确保检测质量控制 验证程序。