Bioassay Core

生物测定中心将为计划项目内的各个项目提供生化测定和基因分型服务。将进行的生化试验包括对人和大鼠样本的血浆和尿液进行分析。将进行以下测定：血浆肾素活性、醛固酮、血管紧张素II、皮质醇、皮质酮、ACTH、亚硝酸盐、硝酸盐、8-异前列烷、TEARS、抗氧化能力、过氧化氢、去甲肾上腺素、肾上腺素、白蛋白、电解质、肌酐、总胆固醇、HDL、LDL、 甘油三酯、葡萄糖、胰岛素、胱抑素C、四氢生物蝶呤、瘦素和转化生长因子β。技术将包括放射免疫测定、放射酶测定、HPLC、ELISA、比色和离子选择性电极。项目2将对GCCR、CRHR 1、p22 phox和EC-SOD基因的标记单核苷酸多态性进行基因分型。将对项目1进行血管紧张素II受体1型基因的C-521 T多态性基因分型。还将使用ETB受体缺陷大鼠和内皮ET敲除小鼠的转基因大鼠的剪尾DMA对项目3和4进行基因分型服务。将对提取的DMA进行基因分型，以确定大鼠的ETB受体基因是野生型、杂合型还是纯合型，以及小鼠的ET基因是野生型、杂合型还是纯合型。该核心还将提供资源，用于开发个体研究者所需的其他检测方法。核心将通过以下方式确保测定质量控制： 验证程序。

The Bioassay Core will provide biochemical assay and genotyping services for the individual projects within the Program Project. Biochemical assays that will be performed include analyses in plasma and urine for human and rat samples. The following assays will be performed: Plasma renin activity, aldosterone, angiotensin II, cortisol, corticosterone, ACTH, nitrite, nitrate, 8-isoprostane, TEARS, antioxidant capacity, hydrogen peroxide, norepinephrine, epinephrine, albumin, electrolytes, creatinine, total cholesterol, HDL, LDL, triglycerides, glucose, insulin, cystatin C, tetrahydrobiopterin, leptin, and transforming growth factor beta. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, ELISA, colorimetric, and ionselective electrodes. Genotyping of tagging single nucleotide polymorphisms of the genes of GCCR, CRHR1, p22phox, and EC-SOD will be performed for Project 2. Genotyping of the C-521T polymorphism of the angiotensin II receptor type 1 gene will be performed for Project 1. Genotyping services will be also performed for Project 3 and 4 using the tail snip DMA of the transgenic rats of the ETB receptor deficient rat and endothelial ET knockout mice. The extracted DMA will be genotyped to determine whether the rats are wildtype, heterozygous, or homozygous for the ETB receptor gene and whether the mice are wild-type, heterozygous, or homozygous for the ET gene. The core will also provide resources for development of additional assays as needed by individual investigators. The core will assure assay quality control through validation procedures.