Bioassay Core

生物分析核心将为计划项目中的个别项目提供生化分析和基因分型服务。将进行的生化分析包括对人和大鼠样本的血浆和尿液分析。将进行以下检测：血浆肾素活性、醛固酮、血管紧张素II、皮质醇、皮质酮、ACTH、亚硝酸盐、硝酸盐、8-异前列腺素、泪液、抗氧化能力、过氧化氢、去甲肾上腺素、肾上腺素、白蛋白、电解质、肌酐、总胆固醇、高密度脂蛋白、低密度脂蛋白、 甘油三酯、葡萄糖、胰岛素、胱抑素C、四氢生物蝶呤、瘦素和转化生长因子β。技术将包括放射免疫分析、放射性酶分析、高效液相色谱法、酶联免疫吸附试验、比色法和离子选择电极。项目2将进行GCCR、CRHR1、p22Phox和EC-SOD基因标记单核苷酸多态的基因分型。项目1将进行血管紧张素II受体1基因C-521T多态的基因分型。项目3和4也将使用ETB受体缺陷大鼠和内皮ET基因敲除小鼠的转基因大鼠的尾部片段DMA进行基因分型服务。提取的DMA将进行基因分型，以确定大鼠是ETB受体基因的野生型、杂合子还是纯合子，小鼠是ET基因的野生型、杂合子或纯合子。该核心还将提供资源，用于开发个别调查人员所需的其他化验方法。核心将通过以下方式确保检测质量控制 验证程序。

The Bioassay Core will provide biochemical assay and genotyping services for the individual projects within the Program Project. Biochemical assays that will be performed include analyses in plasma and urine for human and rat samples. The following assays will be performed: Plasma renin activity, aldosterone, angiotensin II, cortisol, corticosterone, ACTH, nitrite, nitrate, 8-isoprostane, TEARS, antioxidant capacity, hydrogen peroxide, norepinephrine, epinephrine, albumin, electrolytes, creatinine, total cholesterol, HDL, LDL, triglycerides, glucose, insulin, cystatin C, tetrahydrobiopterin, leptin, and transforming growth factor beta. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, ELISA, colorimetric, and ionselective electrodes. Genotyping of tagging single nucleotide polymorphisms of the genes of GCCR, CRHR1, p22phox, and EC-SOD will be performed for Project 2. Genotyping of the C-521T polymorphism of the angiotensin II receptor type 1 gene will be performed for Project 1. Genotyping services will be also performed for Project 3 and 4 using the tail snip DMA of the transgenic rats of the ETB receptor deficient rat and endothelial ET knockout mice. The extracted DMA will be genotyped to determine whether the rats are wildtype, heterozygous, or homozygous for the ETB receptor gene and whether the mice are wild-type, heterozygous, or homozygous for the ET gene. The core will also provide resources for development of additional assays as needed by individual investigators. The core will assure assay quality control through validation procedures.