Spectrometric and Spectroscopic Molecular Pathology and Diagnosis

这项提议试图发现一种基于癌症干细胞的分子特征的疾病分期的新方法。我们建议使用物理技术和样本固有的物理现象作为基础来研究分子病理学的新领域，并将这些技术用作当前病理学的辅助。需要研究相关模型细胞系之间的根本差异，以便能够阐明生化标记以辅助组织病理学。这些技术是非常互补的-SIMS和MIMS是质谱学技术，ATR-IR和拉曼光谱是光谱技术。利用这些技术，我们将涵盖元素(同位素)、分子和大分子信息的全部范围。这将提供翻译分子标记，可以通过更多的临床常规方法进行鉴定，例如本研究阐明的化学物质染色。

This proposal seeks to discover a novel process for staging disease based upon the molecular characteristics of cancer stem cells. We propose to use physical techniques and inherent physical phenomena of the samples as the basis to investigate a new realm for molecular pathology and also to use these techniques as an aid to current pathology.The research is needed into the fundamental differences between the associated model cell lines to enable the elucidation of biochemical markers to aid histopathology. The techniques are very complimentary - SIMS and MIMS are mass spectral techniques and ATR-IR and Raman are spectroscopic. Utilising these techniques we will cover the full range of elemental (isotopic), molecular and macromolecular information. This will provide translational molecular markers which can be identified by more clinically routine methods e.g staining for chemicals elucidated by this study.