Biochemical and Molecular Biology Core Laboratory

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The Biochemical and Molecular Biology Core Laboratory of this Program Project will provide a highly specialized team of professional staff, capable of coordinating a variety of assays in a well-equipped facility. The Core Laboratory will provide Projects I, II and III with the following services: I. Uniform monitoring of sample parameters levels of anesthetic, gas tension, and pH; II. Supply isolated cardiac mitochondria, cardiac myocytes from rat and cultured cells and cells transfected with relevant recombinant cDNAs; IlI. Biochemical evaluation of mitochondrial energetics, protein kinase C, mitogen-activated protein kinase and protein tyrosine kinase activity; and IV. Service for confocal microscopy including mitochondrial flavoprotein fluorescence as well as immunofluorescence. Unlimited access to analytical expertise, highly specialized techniques and equipment in a central facility is absolutely essential to each of the proposed projects. Justification for this Core Laboratory is provided in a listing of the protocols and the number of experiments required for each project. In addition, access is available to a Free Radical Core Facility at the Medical College of Wisconsin.