Biochemical and Molecular Biology Core Laboratory

生化与分子生物学核心实验室

• 批准号: 6584878
• 负责人: MEETHA M MEDHORA
• 金额: $ 23.63万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6584878
• 关键词: acidity /alkalinity; analytical chemistry; animal tissue; biochemistry; biomedical facility; blood chemistry; cardiac myocytes; confocal scanning microscopy; immunofluorescence technique; mitochondria; protein kinase; respiratory gas level; tissue /cell culture; transfection

The Biochemical and Molecular Biology Core Laboratory of this Program Project will provide a highly specialized team of professional staff, capable of coordinating a variety of assays in a well-equipped facility. The Core Laboratory will provide Projects I, II and III with the following services: I. Uniform monitoring of sample parameters levels of anesthetic, gas tension, and pH; II. Supply isolated cardiac mitochondria, cardiac myocytes from rat and cultured cells and cells transfected with relevant recombinant cDNAs; IlI. Biochemical evaluation of mitochondrial energetics, protein kinase C, mitogen-activated protein kinase and protein tyrosine kinase activity; and IV. Service for confocal microscopy including mitochondrial flavoprotein fluorescence as well as immunofluorescence. Unlimited access to analytical expertise, highly specialized techniques and equipment in a central facility is absolutely essential to each of the proposed projects. Justification for this Core Laboratory is provided in a listing of the protocols and the number of experiments required for each project. In addition, access is available to a Free Radical Core Facility at the Medical College of Wisconsin.

本计划项目的生化与分子生物学核心实验室将提供 高度专业化的专业人员团队，能够在设备齐全的设施中协调各种检测。 核心实验室将为项目一、二、三提供以下服务： I. 统一监测麻醉剂、气体张力、pH 等样品参数水平； 二.供应分离的心肌线粒体、大鼠心肌细胞以及培养细胞和细胞 转染相关重组cDNA； 伊尔。线粒体能量学、蛋白激酶 C、丝裂原激活蛋白的生化评估 激酶和蛋白酪氨酸激酶活性；和 四．共聚焦显微镜服务，包括线粒体黄素蛋白荧光以及 免疫荧光。 在中心无限制地获得分析专业知识、高度专业化的技术和设备 设施对于每个拟议项目都是绝对必要的。每个项目所需的实验方案和数量列表中提供了该核心实验室的理由。此外，还可以使用威斯康星医学院的自由基核心设施。