Biochemical and Molecular Biology Core Laboratory

该计划项目的生物化学和分子生物学核心实验室将提供 高度专业化的专业人员团队，能够在设备齐全的设施中协调各种分析。 核心实验室将为项目一、二和三提供下列服务： 对麻醉剂、气体张力和pH值的样本参数水平进行统一监测； 提供分离的大鼠心肌线粒体、心肌细胞和培养的细胞和细胞 转染相应的重组cDNA； 伊利。线粒体能量学、蛋白激酶C、丝裂原活化蛋白的生化评价 激酶和蛋白酪氨酸激酶活性；以及 IV.共聚焦显微镜服务，包括线粒体黄素蛋白荧光以及 免疫荧光。 不受限制地获得分析专业知识、高度专业化的技术和设备 设施对每个拟议的项目都是绝对必要的。每个项目所需的方案和实验次数的清单中提供了该核心实验室的理由。此外，还可以使用威斯康星医学院的自由基核心设施。

The Biochemical and Molecular Biology Core Laboratory of this Program Project will provide a highly specialized team of professional staff, capable of coordinating a variety of assays in a well-equipped facility. The Core Laboratory will provide Projects I, II and III with the following services: I. Uniform monitoring of sample parameters levels of anesthetic, gas tension, and pH; II. Supply isolated cardiac mitochondria, cardiac myocytes from rat and cultured cells and cells transfected with relevant recombinant cDNAs; IlI. Biochemical evaluation of mitochondrial energetics, protein kinase C, mitogen-activated protein kinase and protein tyrosine kinase activity; and IV. Service for confocal microscopy including mitochondrial flavoprotein fluorescence as well as immunofluorescence. Unlimited access to analytical expertise, highly specialized techniques and equipment in a central facility is absolutely essential to each of the proposed projects. Justification for this Core Laboratory is provided in a listing of the protocols and the number of experiments required for each project. In addition, access is available to a Free Radical Core Facility at the Medical College of Wisconsin.