课题基金 / 基金详情

Dynamic Spectroscopic Imaging in Bone Biomechanics

Dynamic Spectroscopic Imaging in Bone Biomechanics
骨生物力学中的动态光谱成像
批准号:
7348387
负责人:
MICHAEL DAVID MORRIS
金额:
$30.14万
依托单位国家:
美国
项目类别:
财政年份:
2005
资助国家:
美国
项目状态:
已结题
起止时间:
2005-01-07 至 2009-11-30
关键词:
AddressAffectAgeAgingAmidesAnimal ModelAnimalsAnionsArchivesAreaBindingBiomechanicsBone DiseasesBone TissueBos taurusCalciumCarbonatesCardiologyCationsCattleCellsCharacteristicsChemical StructureChemicalsChemistryClassificationCleaved cellClinicalCollagenCollagen FibrilCollagen Type ICollectionCompetenceConditionConfidence IntervalsConfocal MicroscopyDataDepthDermatologyDevelopmentEconomic BurdenEnvironmentEnzymesFailureFatigueForce of GravityFractureFrequenciesGenerationsGoalsHelix (Snails)HumanHydrogen BondingImageInorganic ChemistryInterventionInvestigationIonsKnowledgeLasersLife Cycle StagesLightMapsMatrix BandsMeasurementMeasuresMechanical StressMechanicsMedical EconomicsMetabolic Bone DiseasesMetabolic DiseasesMethodsMetricMicroscopyMineralsMolecularMolecular ConformationMolecular StructureMovementMusMusculoskeletalMutationNoiseNumbersOperative Surgical ProceduresOsteoclastsOsteogenesis ImperfectaOsteoporosisOther TherapyOutputPerformancePharmacologic SubstancePhasePhysical environmentPhysiologicalPlasticsPopulationPositioning AttributePrincipal InvestigatorProcessPropertyProteinsRangeResearchResearch PersonnelResolutionRheumatologyRiskRoleRuptureSamplingScientistScoreSecondary Protein StructureSignal TransductionSkeletal systemSocietiesSpecimenSpeedStagingStressStretchingStructureStudy modelsSystemTechniquesTestingTimeTissuesWidthWorkage effectage relatedagedbasebonebone losscrosslinkear helixfallsfluorophorehuman tissueinorganic phosphateinsightinterestmouse modelprogramsresearch studyresponsesizespectroscopic imagingsubstantia spongiosatooltriple helix

项目摘要

项目成果

MICHAEL DAVID MORRIS的其他基金

相似基金

相关文献

中文摘要
翻译
描述(由申请人提供):
英文摘要
DESCRIPTION (provided by applicant): The overall project goals are development of Raman imaging with application to changes in bone mineral crystallite lattice and matrix collagen secondary structure in tissue subjected to tensile and compressive loading. Line-focused 532 nm laser light will be used to excite spectra of tissue pretreated by chemical and photochemical bleaching of fluorophores. An imaging spectrograph and low-light level CCD will provide spatially resolved spectra and ability to measure 0.1-0.2 cm(-1) changes peak in position with integration times as low as 0.05 sec. The project is the first phase of a study of the failure mechanism of osteoporotic tissue at the level of atomic and molecular structure, with the goal of new intervention strategies for minimization of fracture risk. The project will also serve as a model for study of biomechanics and other metabolic diseases and genetic defects of musculoskeletal tissue. Techniques will be developed using bovine bone and then ported to archived human tissue specimens. Our hypotheses are: Ion spacing in bone mineral change under mechanical stress during elastic deformation, while plastic deformation (cracking, compression or fracture) results in permanent changes 2) The matrix responds to mechanical stress in the elastic regime by perturbation of cross-links and changes in helix pitch of the strands of the collagen fibrils, resulting in changes in helix and coil conformations and hydrogen bonding. In plastic deformation cross-links between collagen fibrils are ruptured. These hypotheses can be tested by spectroscopic imaging of tissue because vibratioal frequencies and intensities respond to changes in local environment. Static Raman imaging will explore chemistry of tissue that has been subjected to fatigue loading. Dynamic imaging will follow changes in bone tissue chemical parameters with applied loads. Both univariate and multivariate methods will be used for data reduction. Systematic studies of age effects will be performed on murine tissue with exploratory work on human tissue at age extremes.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Chemical Structure Effects on Bone Response to Mechanical Load
Chemical Structure Effects on Bone Response to Mechanical Load
Chemical Structure Effects on Bone Response to Mechanical Load
Chemical Structure Effects on Bone Response to Mechanical Load
海外基金