Raman Tomography of Musculoskeletal Tissue

肌肉骨骼组织的拉曼断层扫描

• 批准号: 7478574
• 负责人: MICHAEL DAVID MORRIS
• 金额: $ 29.63万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7478574
• 关键词: Algorithms; Analytical Chemistry; Animals; Anterior; Area; Arterial Fatty Streak; Arthroplasty; Bone Diseases; Bone Tissue; Carbonates; Cholesterol; Clinic Visits; Collagen Fibril; Collection; Complex; Consent; DEXA; Defect; Depth; Distal; Family suidae; Fiber; Fiber Optics; Fracture; Fracture Healing; Healed; Health; Human; Image; Imaging Techniques; Invasive; Knee joint; Lasers; Life; Ligaments; Light; Limb structure; Maintenance; Measurement; Measures; Minerals; Modeling; Mus; Musculoskeletal; Operating Room Technicians; Operating Rooms; Operative Surgical Procedures; Optics; Orthopedics; Osteogenesis Imperfecta; Patients; Penetration; Pharmacologic Substance; Procedures; Process; Public Health; Quality Indicator; Raman Spectrum Analysis; Recovery; Replacement Arthroplasty; Research Personnel; Resolution; Risk; Safety; Schedule; Serum; Shapes; Signal Transduction; Site; Skin; Specimen; Spectrum Analysis; Sterility; Stress Fractures; Sus scrofa; Techniques; Technology; Tendon structure; Testing; Time; Tissues; Validation; Week; Wild Type Mouse; animal tissue; bone; bone imaging; bone quality; crosslink; design; detector; experience; fluorescence imaging; healing; human subject; human tissue; inorganic phosphate; instrument; interest; mouse model; new technology; novel; optical fiber; programs; prototype; radius bone structure; research study; spectroscopic imaging; tibia; tissue regeneration; tomography

DESCRIPTION (provided by applicant): We will develop Raman tomography, a non-invasive platform technology, for the recovery of spatial and spectral information from live animals and human subjects. Raman tomography will provide measurements of bone quality at depths of up to 1 cm below the skin. Available quality indicators will include mineral/matrix ratio, mineral crystallinity and carbonate/phosphate ratio and collagen-fibril cross-link state. The technology requires only a CW laser, an imaging spectrograph and CCD detector. Two specially designed fiber optic probes that provide shaped and distributed laser light to excite spectra and an array of 50 or more collection fibers to recover spectra will be used. Guided by tissue optics measurements, a ring/disk probe will be developed for rapid, deep tissue spectroscopy. Similarly, a tomographic probe will be developed for recovery of spectroscopic images. Modified versions of proven chemometric and tomographic signal recovery algorithms will be used to recover spectra and bone quality indicators. We will investigate trade-offs among signal acquisition time, depth penetration and spatial/spectral detail. Optimized probes will be used to study fracture healing in wild type mice and brtl, a model of osteogenesis imperfecta type IV.. Studies will be performed on live anaesthetized mice and on excised limbs from animals sacrificed at four time points in the eight week tissue regeneration period. Excised limbs allow validation by direct measurement of dissected, sectioned tissue. Porcine models will be used as models for human subjects, because of similarities of skin and tendon optics, allowing progression to Raman tomography of cadaveric tibial and distal radius tissue. Finally, we will demonstrate application to human subjects using arthroplasty patients whose bone tissue is exposed during surgery, allowing measurement validation. Although the focus of this project is on bone tissue, the technology is broadly applicable. Relevance to public health: Raman tomography provides new information for assessment of bone quality and fracture risk unavailable by DEXA or micro-MRI. The instruments used are small, safe and non-invasive, requiring only that a laser light be shined on the subject's skin. The technology is also readily adapted to measurement of other important health indicators, including presence and extent of atherosclerotic plaque and levels of serum cholesterol.

描述（由申请人提供）：我们将开发拉曼层析成像，一种非侵入性平台技术，用于从活体动物和人类受试者中恢复空间和光谱信息。拉曼断层扫描将提供皮下1厘米深处的骨质测量。可用的质量指标将包括矿物质/基质比、矿物质结晶度和碳酸盐/磷酸盐比以及胶原纤维交联状态。该技术只需要一个CW激光器，一个成像光谱仪和CCD探测器。将使用两个专门设计的光纤探头，提供成形和分布的激光来激发光谱，并使用50个或更多个收集光纤阵列来恢复光谱。在组织光学测量的指导下，将开发用于快速深层组织光谱的环/盘探头。同样，将开发用于恢复光谱图像的层析成像探头。将使用经过验证的化学计量和断层扫描信号恢复算法的修改版本来恢复光谱和骨质量指标。我们将研究信号采集时间，深度穿透和空间/光谱细节之间的权衡。优化的探针将用于研究野生型小鼠和brtl（一种IV型骨生成模型）中的骨折愈合。将对活的麻醉小鼠和在8周组织再生期的4个时间点处死的动物的切除肢体进行研究。切除的肢体允许通过直接测量解剖的切片组织进行验证。由于皮肤和肌腱光学特性的相似性，猪模型将用作人类受试者的模型，从而可以进行尸体胫骨和桡骨远端组织的拉曼断层扫描。最后，我们将使用在手术过程中暴露骨组织的关节成形术患者来演示对人类受试者的应用，从而允许测量验证。虽然该项目的重点是骨组织，但该技术具有广泛的适用性。 与公共卫生的相关性：拉曼断层扫描为评估骨质量和骨折风险提供了DEXA或micro-MRI无法提供的新信息。所使用的仪器体积小，安全，无创，只需要将激光照射在受试者的皮肤上。该技术也很容易适用于测量其他重要的健康指标，包括动脉粥样硬化斑块的存在和程度以及血清胆固醇水平。