Shared Zeiss 510META Confocal Microscope

描述(由申请人提供)：此申请是为了购买最先进的蔡司共焦显微镜，该显微镜将安装在布朗大学分子医学实验室(LMM)的一座新的生物医学研究大楼中。这套设备将为NIH资助的15名教师提供服务。使用该范围的实验室成员包括学生(研究生、医学、医学博士候选人和本科生)博士后研究员、临床科室的医学研究员和技术人员，总共有60多名潜在用户。新的LMM位于布朗附属医院的研究大楼附近。LMM有三个基础科学和医学院系(病理学系、分子药理学系、生理学和生物技术系以及分子和细胞生物学和生物化学系)。此外，新大楼内还有三个中心，包括遗传学和基因组学中心和一个新的环境健康和技术中心。这种新设备的主要用户群包括LMM的所有部门和中心。将应用新成像技术的研究项目从衰老和阿尔茨海默病的分子基础，到新出现的病原体和毒理学。大多数要成像的生物体需要控制温度以实现生理动态平衡。在这种新的环境下，医学和基础科学专家团队之间的跨学科受精机会是巨大的。活细胞的分子成像已经成为一种如此强大的工具，以至于没有它，大多数基础研究问题都无法得到明确的解决。为了实时记录细胞/分子动力学，需要特殊的设备，如这里提出的共焦显微镜。Meta和多跟踪器扫描头和软件选项提供的多光谱解混功能允许随着时间的推移在同一样本中最多成像八个荧光标记。使用机动的Z-Stage，这种成像可以从5个维度(分子、3D结构和时间推移)捕捉到。这种特殊的显微镜必须放置在离培养/创造活标本的实验室很近的地方，因为这种标本的运输会产生文物。目前，这座新大楼里只有一台过时的共焦，那就是1993年购买的一辆Zeiss410。这项共享仪器的提议是在成像设施中增加一个新的显微镜，这个新的显微镜将由现有的乐德生物医学成像设施和共焦用户委员会管理。相关性：这个拟议的共焦显微镜的主要用户组包括一系列由NIH资助的临床、翻译和基础科学研究人员，他们横跨一系列部门和学科。它们的研究应用解决了与流行疾病有关的基本问题，对公共卫生有重要影响。

DESCRIPTION (provided by applicant): This application is for the purchase of a state-of-the-art Zeiss confocal microscope to be housed in a new Biomedical Research building, the Laboratory of Molecular Medicine (LMM) at Brown University. This equipment will serve 15 NIH-funded faculty. Members of laboratories using the scope include students (graduate, medical, MD-PhD candidates, and undergraduates) post-doctoral PhD fellows, medical fellows from clinical departments, and technical staff, for a total of over 60 potential users. The new LMM is located near research buildings of the Brown affiliated hospitals. LMM houses three basic science and medical school departments (Departments of Pathology, of Molecular Pharmacology, Physiology and Biotechnology, and Molecular and Cellular Biology and Biochemistry). In addition, there are three Centers located in the new building, including Center for Genetics and Genomics and a new Center for Environmental Health and Technology. Major User Group for this new equipment includes all departments and Centers in LMM. The research projects for which the new imaging will be applied span from the molecular basis of aging and Alzheimer's disease, to emerging pathogens, and toxicology. Most organisms to be imaged require temperature control for physiological homeostasis. The opportunities for cross-disciplinary fertilization among teams of medical and basic science experts are immense in this new environment. Molecular imaging of live cells has become such a powerful tool that most basic research questions cannot be addressed definitively without it. For live recording of cellular/molecular dynamics, specialized equipment such as the confocal microscope proposed here is necessary. Multispectral un-mixing provided by the META and multitracker scan head and software options allow up to eight fluorescent markers to be imaged in the same specimen over time. With the motorized z-stage, this imaging is captured in 5 dimensions (molecular, 3D structural and time lapse). This specialized microscope must be housed in close proximity to the laboratories where the living specimens are cultured/created, as transport of such specimens produces artifacts. At present, there is only one out-dated confocal in this new building, a Zeiss410 purchased in 1993. This shared instrumentation proposal is to add a new microscope to the imaging facility at This new microscope will be managed under the existing LeDuc Biomedical Imaging Facility and the Confocal Users Committee. Relevance: The Major Users Group for this proposed confocal microscope includes a range of clinical, translational and basic science NIH-funded investigators spanning a range of departments and disciplines. Their research applications address fundamental questions regarding prevalent disease with important impacts on public health.