BROWN UNIVERSITY CONSORTIUM - PHENOTYPING CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该核心的重点是鉴定骨髓细胞移植后皮肤和骨骼肌细胞的性质。通过共聚焦和反褶积荧光显微镜将确定供体细胞及其特定谱系并进行定殖。供体细胞将在组织切片或细胞悬液中通过GFP、β -半乳糖苷酶的表达或make DNA的存在进行追踪。鉴定供体细胞的谱系将通过标准形态学、地理位置和免疫组织化学来确定。供体和谱系标记的共定位将使用共聚焦或去卷积荧光显微镜确认。核心将为这些方法提供高级专业知识。核心包括两台蔡司荧光电机驱动显微镜，一台直立的Axioplan 2和一台倒置的Axiovert 200M，以及一台能够进行四色成像的蔡司510共聚焦激光扫描显微镜。核心将能够进行标准的反褶积荧光显微镜，3D重建和共聚焦显微镜。这些后一种，加上照相，用于同时显示供体标记和细胞类型特异性标记。核心课程对COBRE参与者开放，不收费。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core focuses on identifying the nature of cells in skin and skeletal muscle after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocialized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of make DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemsitry. Colocalization of donor and lineage markers will be confirmed using confocal or deconbolution fluorescent microscopy. The core will provide high-level expertise for these approaches. The core contains two Zeiss fluorescent motor-driven microscopes, an upright Axioplan 2 and an inversted Axiovert 200M, and a Zeiss 510 confocal laser-scanning microscope capable of four-color imaging. The core will be capable of standard deconvolution fluorescent microscopy, 3D reconstructions and confocal microscopy. These latter, plus photomapping are used to simultaneously show a donor marker and a cell type specific marker. The core is open to COBRE participants and there are no charges.