BROWN UNIVERSITY CONSORTIUM - PHENOTYPING CORE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core focuses on identifying the nature of cells in skin and skeletal muscle after marrow cells transplantation. The overall plan has been for donor cells and their specific lineages to be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. In this plan, donor cells can be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of make DNA. The lineage of identified donor cells can be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers canbe confirmed using confocal or deconvolution fluorescent microscopy. The core canprovide high-level expertise for these approaches. The core contains two Zeiss fluorescent motor-driven microscopes, an upright Axioplan 2 and an inverted Axiovert 200M, and a Zeiss 510 confocal laser-scanning microscope capable of four-color imaging. The core is capable of standard deconvolution fluorescent microscopy, 3D reconstructions and confocal microscopy. These latter, plus photomapping can be used to simulateously show a donor marker and a cell type specific marker.