MULTI-PHOTON CONFOCAL MICROSCOPE SYSTEM

多光子共焦显微镜系统

• 批准号: 7389041
• 负责人: JANOS PETI-PETERDI
• 金额: $ 50万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389041
• 关键词: Animals; Applications Grants; Arts; Cell physiology; Clinical; Core Facility; Data; Depth; Diabetes Mellitus; Disease; Equipment; Fluorescence; Funding; Grant; Health; Health Sciences; Image; Immunology; Institutes; Laboratories; Life; Medicine; Microbiology; Microscope; Microscopy; Molecular Neurobiology; Neck; Numbers; Optics; Organ; Other Imaging Modalities; Pilot Projects; Postdoctoral Fellow; Publications; Recruitment Activity; Research; Research Personnel; Resolution; Resources; Scanning; Science; Services; System; Technical Expertise; Technology; Time; Translational Research; United States National Institutes of Health; Universities; base; bioimaging; desire; in vivo; instrument; medical schools; multi-photon; success

DESCRIPTION (provided by applicant): This request is for funds to purchase a multi-photon Leica TCS SP5 fluorescence scanning confocal microscope. This will be a dedicated core equipment for the formally established Multi-Photon Core at the USC Keck School of Medicine in the Health Sciences Campus. The core is an integral part of, and funded by the USC Biomedical Imaging Science Initiative (BISI). The mandate of this new core facility is to provide access to multi- photon in vivo imaging of intact organs in small animals which is a long-desired, but still missing service on campus. This state-of-the-art technology is recognized for its capability of deep optical sectioning of living organs. It will enable USC investigators to perform ultra-sensitive, quantitative imaging of organ functions in health and disease with high spatial and temporal resolution, that other imaging modalities can not achieve. This critically needed instrument is essential to continue real core services which have been recently expanded due to the great success of the BISI. Janos Peti-Peterdi, the PI on this application, was recruited to USC in 2004 for his expertise in multi- photon imaging. In the past three years, Dr. Peti-Peterdi and his laboratory assisted a great number of HSC investigators to obtain preliminary data and images for grant proposals and scientific publications using his individually owned and maintained multi-photon microscope. This voluntary service, providing free access to, and supervised, assisted use of his own microscope was the basis of formally establishing the Multi-Photon Core. As the first step in transforming this function into a more robust, University wide resource, the BISI recently provided a full-time research associate for the core. This RA provides technical expertise to a growing number of investigators through BISI pilot projects. At the same time, Dr. Peti-Peterdi significantly expanded his own research so that his NIH, AHA-funded projects now require full capacity of his own microscope. There is clearly a critical need for a dedicated core equipment, and obtaining this shared instrument will be the final step of becoming a real Multi-Photon Core. As the PHS funded user base continues to expand, a lack of available multi-photon microscope time has become a major limiting factor in the utility of the core. The acquisition of the requested instrument will allow us to match resources of the core with the expanding demand for access to multi-photon in vivo organ microscopy, relieving the bottle neck that currently impedes scientific projects. Multi-photon microscopy of intact organs is an essential service provided by the core to many HSC investigators from the Departments of Medicine, Physiology, Cell and Neurobiology, Molecular Microbiology, and Immunology with existing, pending and renewal PHS grants. This multi-photon core is also critically needed for the USC Diabetes Research Center and the Clinical and Translational Science Institute, both being established pending PHS grants.

说明（由申请人提供）：本申请用于购买多光子徕卡TCS SP5荧光扫描共聚焦显微镜。这将是南加州大学凯克医学院健康科学校区正式建立的多光子核心的专用核心设备。核心是USC生物医学成像科学计划（BISI）的一个组成部分，并由其资助。这个新的核心设施的任务是提供小动物完整器官的多光子体内成像，这是一个长期渴望的，但在校园内仍然缺乏的服务。这项最先进的技术因其对活体器官进行深度光学切片的能力而得到认可。它将使南加州大学的研究人员能够以高空间和时间分辨率对健康和疾病中的器官功能进行超灵敏的定量成像，这是其他成像方式无法实现的。这一急需的工具对于继续提供真正的核心服务至关重要，这些服务最近由于BISI的巨大成功而得到了扩展。Janos Peti-Peterdi，这个项目的负责人，在2004年被招募到南加州大学，因为他在多光子成像方面的专长。在过去的三年里，Peti-Peterdi博士和他的实验室使用他个人拥有和维护的多光子显微镜，帮助大量HSC研究人员获得初步数据和图像，用于资助提案和科学出版物。这项志愿服务，提供免费使用，并监督和协助使用他自己的显微镜，是正式建立多光子核心的基础。作为将这一功能转变为更强大的全校资源的第一步，BISI最近为核心提供了一名全职研究助理。该RA通过BISI试点项目为越来越多的研究人员提供技术专长。与此同时，佩蒂-彼得迪博士大大扩展了他自己的研究，因此他的NIH和aha资助的项目现在需要他自己的显微镜的全部容量。显然，迫切需要一个专用的核心设备，而获得这个共享的仪器将是成为一个真正的多光子核心的最后一步。随着小灵通资助的用户群不断扩大，缺乏可用的多光子显微镜时间已成为核心效用的主要限制因素。获得所要求的仪器将使我们能够将核心资源与对获得多光子体内器官显微镜的不断扩大的需求相匹配，从而缓解目前阻碍科学项目的瓶颈。完整器官的多光子显微镜是由医学、生理学、细胞和神经生物学、分子微生物学和免疫学等部门的许多HSC研究人员提供的一项基本服务，这些部门现有的、正在申请的和正在更新的PHS拨款。南加州大学糖尿病研究中心和临床与转化科学研究所也迫切需要这种多光子核心，这两个研究中心都在等待PHS拨款。