Multiphoton microscope replacement for USC shared resource

多光子显微镜替代南加州大学共享资源

• 批准号: 9274881
• 负责人: JANOS PETI-PETERDI
• 金额: $ 59.98万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274881
• 关键词: Animals; Biological Markers; Biomedical Engineering; Biomedical Research; Brain; Cost Sharing; Degenerative Disorder; Dental; Devices; Disease; Equipment; Funding; Gerontology; Health; Housing; Human; Image; Institutes; Kidney; Lacrimal gland structure; Lasers; Liver Stem Cell; Lung; Maintenance; Malignant neoplasm of brain; Manufacturer Name; Microscope; Microscopy; Modeling; Organ; Pharmacologic Substance; Pharmacy Schools; Regenerative Medicine; Research; Research Activity; Research Personnel; Research Support; Resource Sharing; School Dentistry; Schools; Science; Services; Stem cells; System; Technology; Testing; Tissue imaging; Tissues; United States National Institutes of Health; base; cellular imaging; imaging modality; imaging study; improved; in vivo; instrumentation; intravital imaging; medical schools; multi-photon; neurogenetics; novel; operation; quantitative imaging; repaired; temporal measurement

MULTIPHOTON MICROSCOPE REPLACEMENT FOR USC SHARED RESOURCE This proposal is submitted to purchase a new, high-end Leica SP8 AOBS multiphoton microscope with 1300nm IR laser for the Multi-Photon Microscopy Core at the Keck School of Medicine at USC. The new microscope will replace an old, non-operational model which is beyond repair and no longer serviced by the manufacturer. The new microscope will be a unique system dedicated to provide ultra-sensitive, deep tissue imaging of intact living organs in vivo in small animals with high spatial and temporal resolution that other intravital imaging modalities cannot achieve. The new core microscope is critically needed by a large research base of 14 NIH-funded investigators from four USC schools/campuses including the Keck School of Medicine, School of Pharmacy, Davis School of Gerontology, and the Herman Ostrow School of Dentistry. The proposed equipment will be used by these core investigators from seven major user groups/themes, including kidney, liver, stem cells, lung & lacrimal gland, brain, cancer, and dental imaging. The new microscope will make a substantial difference in USC's research activities and fulfill an unmet need for deep tissue intravital quantitative imaging for the study of organ functions in vivo in health and disease. The Zilkha Neurogenetic Institute, which is the academic unit housing this core is fully committed to the ongoing maintenance and operation of this new multiphoton microscope. The USC Multi-Photon Microscopy Core is an integral part of the Center of Excellence in Cell & Tissue Imaging, and the new microscope purchase will meet a strategic need expressed in the USC Plan for Science and Technology Facilities by providing instrumentation and collaborative expertise for biomedical and engineering research aimed at improving human health. Therefore, the USC Core Instrumentation Fund is providing significant cost-share ($200,000) toward the acquisition of this new microscope. Research supported by this core and new instrumentation will lead to our better understanding of the miracle of the brain, the normal functioning of the body's organs, the discovery of disease mechanisms and biomarkers, testing novel pharmaceuticals and biomedical devices, and the application of stem cells and regenerative medicine to cure degenerative disorders, and will help to keep USC at the leading edge of biomedical research.

为南加州大学共享资源更换多光子显微镜 这份提案是为了购买一台新的高端徕卡SP8 AOBS多光子 1300 nm红外激光在Keck多光子显微镜核心上的应用 南加州大学医学院。这台新显微镜将取代一台无法操作的旧显微镜 已无法维修且不再由制造商提供服务的型号。新的 显微镜将是一种独特的系统，致力于提供超敏感、深层组织 高时空分辨率小动物活体完整器官的成像 分辨率是其他生命内成像设备无法达到的。新的核心 由NIH资助的14名研究人员组成的大型研究基地迫切需要显微镜 来自南加州大学的四所学校/校区，包括凯克医学院、 药学、戴维斯老年学学院和赫尔曼·奥斯特罗牙科学院。 拟议的设备将由这些核心调查人员使用，他们来自七个主要 用户群/主题，包括肾、肝、干细胞、肺和泪腺、脑、 癌症和牙科成像。这种新显微镜将在 南加州大学的研究活动和满足深部组织活体内定量的未满足需求 用于研究健康和疾病体内器官功能的成像技术。齐尔喀人 神经遗传学研究所，这是这个核心的学术单位，完全致力于 这台新的多光子显微镜正在进行维护和操作。南加州大学 多光子显微镜核心是细胞和技术卓越中心的一个组成部分 组织成像和新的显微镜采购将满足战略需求 在南加州大学科学和技术设施计划中通过以下方式表达 生物医学和工程研究的仪器和协作专业知识 旨在改善人类健康。因此，南加州大学核心仪器基金是 为收购这一新产品提供可观的成本份额(200,000美元) 显微镜。由这一核心和新仪器支持的研究将导致我们的 更好地理解大脑的奇迹，身体的正常运作 器官，疾病机制和生物标记物的发现，测试新书 药物和生物医学设备，以及干细胞和 治疗退行性疾病的再生医学，将有助于使南加州大学保持在 生物医学研究的前沿。

项目成果

Aldosterone induces albuminuria via matrix metalloproteinase-dependent damage of the endothelial glycocalyx.

醛固酮通过基质金属蛋白酶依赖性糖蛋白糖脂诱导蛋白尿。

• DOI: 10.1016/j.kint.2018.08.024
• 发表时间: 2019-01
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: Butler MJ;Ramnath R;Kadoya H;Desposito D;Riquier-Brison A;Ferguson JK;Onions KL;Ogier AS;ElHegni H;Coward RJ;Welsh GI;Foster RR;Peti-Peterdi J;Satchell SC
• 通讯作者: Satchell SC

New Endothelial Mechanisms in Glomerular (Patho)biology and Proteinuria Development Captured by Intravital Multiphoton Imaging.

• DOI: 10.3389/fmed.2021.765356
• 发表时间: 2021
• 期刊: Frontiers in medicine
• 影响因子: 3.9
• 作者: Gyarmati G;Jacob CO;Peti-Peterdi J
• 通讯作者: Peti-Peterdi J

Phenotypic dissection of the mouse Ren1d knockout by complementation with human renin.

通过与人肾素的互补来对小鼠REN1D敲除的表型解剖。

• DOI: 10.1074/jbc.ra117.000160
• 发表时间: 2018-01-26
• 期刊: The Journal of biological chemistry
• 作者: Buckley C;Nelson RJ;Mullins LJ;Sharp MGF;Fleming S;Kenyon CJ;Semprini S;Steppan D;Peti-Peterdi J;Kurtz A;Christian H;Mullins JJ
• 通讯作者: Mullins JJ

Intravital imaging reveals glomerular capillary distension and endothelial and immune cell activation early in Alport syndrome.

• DOI: 10.1172/jci.insight.152676
• 发表时间: 2022-01-11
• 期刊: JCI insight
• 影响因子: 8
• 作者: Gyarmati G;Shroff UN;Izuhara A;Hou X;Da Sacco S;Sedrakyan S;Lemley KV;Amann K;Perin L;Peti-Peterdi J
• 通讯作者: Peti-Peterdi J

A new view of macula densa cell protein synthesis.

• DOI: 10.1152/ajprenal.00222.2021
• 发表时间: 2020-10
• 期刊: American journal of physiology. Renal physiology
• 作者: U. Shroff;Georgina Gyarmati;Audrey Izuhara;Sachin Deepak;J. Peti-Peterdi