Confocal Microscope

共焦显微镜

• 批准号: 7383535
• 负责人: Mark R Ackermann
• 金额: $ 43.39万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2010-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7383535
• 关键词: Age; Annual Reports; Arts; Biotechnology; Cells; Chromatin Structure; Confocal Microscopy; Fascicle; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Gene Expression; Gene Targeting; Housing; Image Analysis; Iowa; Life; Light; Maintenance; Microscope; Minor; Neurons; Oxidative Stress; Phosphotransferases; Public Health; Range; Rate; Research Personnel; Resolution; Retrotransposon; Scanning; Signal Transduction; Stem Cell Development; Stifle joint; Surveys; System; Training; United States National Institutes of Health; Universities; aptamer; cellular imaging; experience; instrumentation; radius bone structure; reconstruction

DESCRIPTION (provided by applicant): State-of-the art confocal microscopy is required for NIH-funded projects at Iowa State University (ISU). The existing multi-user confocal system at ISU is housed and operated by the Image Analysis and Confocal Microscopy Facility, but a new system is needed, because: 1) the existing Leica System TCS/NT (1998) is aging, cannot handle the live cell imaging needs of major users, and lacks needed accessories such as FRET and 3D- reconstruction, and 2) no other confocal system with these capabilities is available to ISU investigators for a 100 mile radius. Through survey and discussion, we have determined that a Leica TCS SP5 would provide the fast scan rates, high resolution, high sensitivity to reduce light load and damage to cells, and ability to fine tune excitation and emission channels to aid in simultaneous acquisition of multiple fluorescence channels needed for five major NIH-funded projects as well as minor projects, some also NIH-funded, outlined in this proposal. These investigators have much experience in confocal microscopy, but are stifled by the limitations of our existing system. Major user projects include: axonal fascicle formation, JIL-1 kinase effects on chromatin structure and gene expression, PKC4 signaling, oxidative stress, and dopaminergic degeneration in neurons, aptamer tags for assessing gene expression and neuronal stem cell development and plasticity, and retrotransposon gene targeting. The Image Analysis and Confocal Facility at ISU is also well suited to handle this new system for the major and minor users. The facility is supported by the Office of Biotechnology at ISU, has existed since 1984. Ackermann (PI) with Carter (collaborator) acquired, operated, and managed the existing confocal system since 1998. Ackermann is a minor user in this proposal, but has an R01- funded project. The facility handles all aspects of the confocal system (maintenance, training, usage, upgrades, etc.) and has had an active user committee since inception and provides annual reports to the Council of the Office of Biotechnology. This instrumentation is relevant to public health in terms of supporting already funded NIH- projects that range from a better understanding of gene expression to neuronal stem cell development and plasticity.

描述（由申请人提供）：爱荷华州立大学 (ISU) 的 NIH 资助项目需要最先进的共聚焦显微镜。 ISU 现有的多用户共焦系统由图像分析和共焦显微镜设施安置和操作，但需要一个新系统，因为：1) 现有的 Leica 系统 TCS/NT (1998) 已经老化，无法满足主要用户的活细胞成像需求，并且缺乏 FRET 和 3D 重建等所需附件，2) ISU 研究人员在 100 年内没有其他具有这些功能的共焦系统可用。 英里半径。通过调查和讨论，我们确定 Leica TCS SP5 将提供快速扫描速率、高分辨率、高灵敏度，以减少光负载和对细胞的损伤，并能够微调激发和发射通道，以帮助同时采集本提案中概述的 5 个 NIH 资助的主要项目以及 NIH 资助的小型项目（其中一些项目也是 NIH 资助的）所需的多个荧光通道。这些研究人员在共焦显微镜方面拥有丰富的经验，但受到我们现有系统的局限性的限制。主要用户项目包括：轴突束形成、JIL-1激酶对染色质结构和基因表达的影响、PKC4信号传导、氧化应激和神经元多巴胺能变性、用于评估基因表达和神经元干细胞发育和可塑性的适体标签，以及逆转录转座子基因靶向。 ISU 的图像分析和共焦设施也非常适合为主要和次要用户处理这个新系统。该设施由 ISU 生物技术办公室支持，自 1984 年以来一直存在。自 1998 年以来，Ackermann（PI）与 Carter（合作者）收购、操作和管理现有的共聚焦系统。Ackermann 是该提案中的次要用户，但有一个 R01 资助的项目。该设施处理共聚焦系统的各个方面（维护、培训、使用、升级等），自成立以来一直有一个活跃的用户委员会，并向生物技术办公室理事会提供年度报告。该仪器与公共健康相关，支持已资助的 NIH 项目，这些项目范围从更好地理解基因表达到神经元干细胞的发育和可塑性。