Becton Dickinson LSR-II Analytical Cytometer (BD-LSR-II)

描述(由申请人提供)：新的基因技术，结合特征良好的基因组，使小鼠成为人类疾病的首选哺乳动物模型。具有遗传预定病理生物学综合征的小鼠品系的产生和特征对于生物医学研究是至关重要的。杰克逊实验室是小鼠遗传学研究和人类疾病小鼠模型开发的领先中心，这些疾病包括癌症、糖尿病、自身免疫性疾病、年龄相关疾病、血液疾病和神经退行性疾病。为了充分开发和利用这些模型的生物医学潜力，杰克逊的研究人员必须获得关键的尖端技术，以便在细胞水平上对这些模型进行全面的遗传和表型表征。多色分析流式细胞术是表型和识别低频率细胞亚群的重要表征工具。杰克逊的研究目前受限于FACSVantage DIVA细胞分选机的8色最大值以及对该仪器日益增长的需求。升级分选机以适应更多的颜色是不切实际的；此外，使用FACSVantage进行分析实验会阻碍需要其分选功能的研究项目。 需要为具有13色能力的最先进的Becton-Dickinson LSR II分析细胞仪提供资金，这将增加表型和种群鉴定实验的特异性、范围和准确性，同时减少所需的样本量，从而减少分析所需的小鼠数量。BD-LSR-II实现了更高的数据分辨率和重复性，以及减少了实验时间和成本。与分选机不同，经过最少技术培训的研究人员可以独立操作BD-LSR-II，将FACSVantage和技术人员从分析实验中解放出来，并最大限度地提高分选和分析能力。购买BD-LSR-II是一种具有成本效益的方式，可以提高流式细胞术服务的能力和效率，利用NIH资助的16项拨款解决11名杰克逊研究人员未得到满足的研究需求，并增强小鼠模型数据库和存储库，使整个生物医学界受益。

DESCRIPTION (provided by applicant): New genetic technologies, combined with a well-characterized genome, make the mouse the preferred mammalian model for human disease. The generation and characterization of mouse strains harboring genetically predetermined pathobiological syndromes is critical to biomedical research. The Jackson Laboratory is the leading center of mouse genetics research and the development of mouse models for human diseases including cancer, diabetes, autoimmune diseases, age-related diseases, blood disorders, and neurodegenerative disorders. To fully develop and exploit the biomedical potential of these models, Jackson researchers must have access to critical cutting-edge technologies that allow comprehensive genetic and phenotypic characterization of these models at the cellular level. Multicolor analytical flow cytometry is an essential characterization tool for phenotyping and identifying low-frequency cell subpopulations. Research at Jackson is currently limited by the 8-color maximum of the FACSVantage DiVa cell sorter and increasing demand on this instrument. It is not practical to upgrade the sorter to accommodate additional colors; furthermore, using the FACSVantage for analytical experiments impedes research projects that require its sorting capabilities. Funding is requested for a state-of-the-art Becton-Dickinson LSR II analytical cytometer with 13-color capability, which will increase the specificity, scope, and accuracy of phenotyping and population identification experiments while reducing the amount of sample needed, thereby decreasing the number of mice required for analyses. The BD-LSR-II achieves improved data resolution and reproducibility, as well as reduced experiment times and cost. Unlike the sorter, researchers with minimal technical training can independently operate the BD-LSR-II, freeing the FACSVantage and the technician from analytical experiments and maximize both sorting and analytical capabilities. Purchase of the BD-LSR-II is a cost-effective way to increase the power and efficiency of the Flow Cytometry Service, address unmet research needs of 11 Jackson investigators with 16 NIH funded grants, and enhance mouse model databases and repositories that benefit the entire biomedical community.