Becton Dickinson LSR-II Analytical Cytometer (BD-LSR-II)

描述（由申请人提供）：新的基因技术，结合良好特征的基因组，使小鼠成为人类疾病的首选哺乳动物模型。具有遗传预定病理综合征的小鼠品系的产生和表征对生物医学研究至关重要。杰克逊实验室是小鼠遗传学研究和人类疾病小鼠模型开发的领先中心，包括癌症、糖尿病、自身免疫性疾病、年龄相关疾病、血液疾病和神经退行性疾病。为了充分开发和利用这些模型的生物医学潜力，Jackson研究人员必须获得关键的尖端技术，以便在细胞水平上对这些模型进行全面的遗传和表型表征。多色流式细胞术是一种重要的表征工具，用于表型和识别低频细胞亚群。杰克逊的研究目前受到facadvantage DiVa细胞分选器8色最大值的限制，并且对该仪器的需求不断增加。升级分选机以适应额外的颜色是不实际的；此外，在分析实验中使用facadvantage会阻碍需要其分选能力的研究项目。

DESCRIPTION (provided by applicant): New genetic technologies, combined with a well-characterized genome, make the mouse the preferred mammalian model for human disease. The generation and characterization of mouse strains harboring genetically predetermined pathobiological syndromes is critical to biomedical research. The Jackson Laboratory is the leading center of mouse genetics research and the development of mouse models for human diseases including cancer, diabetes, autoimmune diseases, age-related diseases, blood disorders, and neurodegenerative disorders. To fully develop and exploit the biomedical potential of these models, Jackson researchers must have access to critical cutting-edge technologies that allow comprehensive genetic and phenotypic characterization of these models at the cellular level. Multicolor analytical flow cytometry is an essential characterization tool for phenotyping and identifying low-frequency cell subpopulations. Research at Jackson is currently limited by the 8-color maximum of the FACSVantage DiVa cell sorter and increasing demand on this instrument. It is not practical to upgrade the sorter to accommodate additional colors; furthermore, using the FACSVantage for analytical experiments impedes research projects that require its sorting capabilities. Funding is requested for a state-of-the-art Becton-Dickinson LSR II analytical cytometer with 13-color capability, which will increase the specificity, scope, and accuracy of phenotyping and population identification experiments while reducing the amount of sample needed, thereby decreasing the number of mice required for analyses. The BD-LSR-II achieves improved data resolution and reproducibility, as well as reduced experiment times and cost. Unlike the sorter, researchers with minimal technical training can independently operate the BD-LSR-II, freeing the FACSVantage and the technician from analytical experiments and maximize both sorting and analytical capabilities. Purchase of the BD-LSR-II is a cost-effective way to increase the power and efficiency of the Flow Cytometry Service, address unmet research needs of 11 Jackson investigators with 16 NIH funded grants, and enhance mouse model databases and repositories that benefit the entire biomedical community.