Acquisition of a Cryoprobe for an 800 MHz NMR Spectrometer

说明（由申请人提供）：请求提供支持，以购买用于现有800 MHz NMR光谱仪的冷冻探针，该光谱仪专用于蛋白质和海洋天然产品的溶液状态NMR研究。通过显著提高UCSD校园内最高场溶液状态NMR光谱仪的灵敏度，冷冻探针将对生物医学研究产生广泛的影响。所要求的仪器不仅将通过其对1H和13C检测的高灵敏度和分辨率的组合使新的实验类别变得可行，而且还将通过有效地加倍（或更多）可以在仪器上进行的实验数量来大大提高研究小组的整体生产力，其中对时间的要求超过了每个月的时间表。所有NIH支持的UCSD研究人员，其研究涉及生物分子溶液状态NMR光谱学，都为这一提议做出了贡献。随着斯克里普斯海洋学研究所和新药学院的研究人员的加入，仪器将加强NIH在生物医学研究的两个主要领域支持的项目：结构生物学和海洋天然产品，为治疗疾病的新药提供补充途径。仪器与生物医学研究的相关性主要体现在两个方面。主要用户涉及结构生物学，特别是蛋白质的结构，动力学和折叠的研究，以及通过海洋天然产物化学的研究进行药物发现。基于结构的药物设计和基于药物化学的药物设计的组合有可能导致癌症，艾滋病和其他传染病以及炎症性疾病等多种疾病的新疗法。

DESCRIPTION (provided by applicant): Support is requested to purchase a cryoprobe for an existing 800 MHz NMR spectrometer dedicated to solution-state NMR studies of proteins and marine natural products. By significantly improving the sensitivity of the highest field solution-state NMR spectrometer on the UCSD campus, the cryoprobe will have a broad impact on biomedical research. Not only will the requested instrumentation make new classes of experiments feasible through its combination of high sensitivity and resolution for both 1H and 13C detection, but also it will greatly enhance the overall productivity of the research groups by effectively doubling (or more) the number of experiments that can be performed on an instrument where the requests for time exceed the time available on each monthly schedule. All NIH-supported UCSD investigators whose research involves biomolecular solution-state NMR spectroscopy have contributed to this proposal. With the inclusion of researchers from the Scripps Institute of Oceanography and the new Pharmacy School, the instrumentation will enhance NIH-supported projects in two principal areas of biomedical research: structural biology and marine natural products, which provide complementary routes to new drugs for the treatment of diseases. The relevance of the instrumentation to biomedical research is in two principal areas. The Major Users are involved in structural biology, in particular studies of the structure, dynamics, and folding of proteins, and in drug discovery through the investigation of marine natural products chemistry. The combination of structure-based and medicinal-chemistry based drug design has the potential to lead to new therapeutics for diseases as diverse as cancer, AIDS and other infectious diseases, and inflammatory diseases.