Acquisition of a Cryoprobe for an 800 MHz NMR Spectrometer

描述(由申请人提供)：请支持为现有的800 MHz核磁共振波谱仪购买低温探头，该波谱仪专门用于蛋白质和海洋天然产品的溶液状态核磁共振研究。通过显著提高加州大学圣迭戈分校最高场溶液状态核磁共振光谱仪的灵敏度，该低温探头将对生物医学研究产生广泛影响。所要求的仪器不仅将通过结合1H和13C检测的高灵敏度和分辨率而使新的实验类别可行，而且它还将通过有效地增加(或更多)可在仪器上进行的实验数量而极大地提高研究小组的整体生产力，如果仪器上的时间请求超过每个月时间表上可用的时间。所有由NIH支持的UCSD研究人员都对这一提议做出了贡献，他们的研究涉及生物分子溶液状态核磁共振光谱。随着斯克里普斯海洋研究所和新药学院的研究人员的加入，该仪器将加强NIH支持的两个主要生物医学研究领域的项目：结构生物学和海洋天然产品，这两个领域为治疗疾病的新药提供补充途径。该仪器与生物医学研究的相关性主要体现在两个方面。主要用户涉及结构生物学，特别是蛋白质的结构、动力学和折叠的研究，以及通过海洋天然产物化学研究的药物发现。基于结构的药物设计和基于药物化学的药物设计相结合，有可能为癌症、艾滋病和其他传染病以及炎症性疾病带来新的治疗方法。

DESCRIPTION (provided by applicant): Support is requested to purchase a cryoprobe for an existing 800 MHz NMR spectrometer dedicated to solution-state NMR studies of proteins and marine natural products. By significantly improving the sensitivity of the highest field solution-state NMR spectrometer on the UCSD campus, the cryoprobe will have a broad impact on biomedical research. Not only will the requested instrumentation make new classes of experiments feasible through its combination of high sensitivity and resolution for both 1H and 13C detection, but also it will greatly enhance the overall productivity of the research groups by effectively doubling (or more) the number of experiments that can be performed on an instrument where the requests for time exceed the time available on each monthly schedule. All NIH-supported UCSD investigators whose research involves biomolecular solution-state NMR spectroscopy have contributed to this proposal. With the inclusion of researchers from the Scripps Institute of Oceanography and the new Pharmacy School, the instrumentation will enhance NIH-supported projects in two principal areas of biomedical research: structural biology and marine natural products, which provide complementary routes to new drugs for the treatment of diseases. The relevance of the instrumentation to biomedical research is in two principal areas. The Major Users are involved in structural biology, in particular studies of the structure, dynamics, and folding of proteins, and in drug discovery through the investigation of marine natural products chemistry. The combination of structure-based and medicinal-chemistry based drug design has the potential to lead to new therapeutics for diseases as diverse as cancer, AIDS and other infectious diseases, and inflammatory diseases.